Procedure
Alexandra Pentel
Developing an Environmentally Friendly Pesticide
Procedure
In order to begin, four vials of wild type Drosophila melanogaster, a set of vials, netting, sponge plugs, media, yeast, and FlyNap® were obtained from Carolina Biological Supply Company. Catalog numbers 172100 and 171905 were ordered which included the various fly vials and a basic fly care set. All experiments were conducted at Dr. Halloran’s school lab at 7400 San Jose Boulevard, Jacksonville, FL 32217. All usage of FlyNap® occurred under a ventilation hood in the lab. Before experimentation with flies began, vials were prepared with media. The first group, the control, had two vials and was labeled with pink tape. The media for these vials was prepared in a normal manner: media to water ratios of 1:1. A second group was prepared with a media to water ration of 1:1 and labeled with blue tape. The blue tape vials would have soy added to them later. The last group was labeled with white tape and had media to water to soy ratios of 1:1:0.5. Netting was then added to the vials for the flies to climb on. Finally, yeast was sprinkled on top of the media, as directed by the fly culturing kit. Flies from two vials of Drosophila melanogaster were anesthetized using FlyNap® and transferred onto a piece of construction paper. The anesthetized flies were then transferred into vials in approximately equal amounts by rolling the paper and letting them slide into the vials. Sponge topper plugs were then put into place and the flies were left to mate until a sufficient amount of pupa could be observed. After a sufficient amount of pupa and larva could be observed in all of the vials, the adult flies were anesthetized, and removed from the containers. Once removed, the sponge stoppers were put back into place. The removed adults were counted, recorded, and put in plastic ZipLock bags to be put into the lab refrigerator. A pinch of the powdered soy milk used in the white labeled vials was then
Developing an Environmentally Friendly Pesticide
Procedure
In order to begin, four vials of wild type Drosophila melanogaster, a set of vials, netting, sponge plugs, media, yeast, and FlyNap® were obtained from Carolina Biological Supply Company. Catalog numbers 172100 and 171905 were ordered which included the various fly vials and a basic fly care set. All experiments were conducted at Dr. Halloran’s school lab at 7400 San Jose Boulevard, Jacksonville, FL 32217. All usage of FlyNap® occurred under a ventilation hood in the lab. Before experimentation with flies began, vials were prepared with media. The first group, the control, had two vials and was labeled with pink tape. The media for these vials was prepared in a normal manner: media to water ratios of 1:1. A second group was prepared with a media to water ration of 1:1 and labeled with blue tape. The blue tape vials would have soy added to them later. The last group was labeled with white tape and had media to water to soy ratios of 1:1:0.5. Netting was then added to the vials for the flies to climb on. Finally, yeast was sprinkled on top of the media, as directed by the fly culturing kit. Flies from two vials of Drosophila melanogaster were anesthetized using FlyNap® and transferred onto a piece of construction paper. The anesthetized flies were then transferred into vials in approximately equal amounts by rolling the paper and letting them slide into the vials. Sponge topper plugs were then put into place and the flies were left to mate until a sufficient amount of pupa could be observed. After a sufficient amount of pupa and larva could be observed in all of the vials, the adult flies were anesthetized, and removed from the containers. Once removed, the sponge stoppers were put back into place. The removed adults were counted, recorded, and put in plastic ZipLock bags to be put into the lab refrigerator. A pinch of the powdered soy milk used in the white labeled vials was then