Investigation in Microtubule Dynamic Instability
Title: Investigation in microtubule dynamic instability
Introduction
Microtubules are important for maintaining cell structure, intracellular transport, formation of mitotic spindle, as well as other cellular processes. Investigation of dynamics of microtubule assembly and disassembly allow us to understand the malfunction of mitotic spindle formation or other cellular processes. This experiment is divided into two parts; we are going to find out the critical parameters for achieving greatest average length of microtubules in part one and achieving the greatest number of microtubules in part two.
Principle
In this experiment, we used a simulation programme to explore how various factors change the way microtubules grow out from centrosome, and the shrink back. Growth rate, shrink rate, catastrophe rate, rescue rate, release rate, minus end end depolymerization rate, nucleation rate and nucleation site are the factors we can adjust to see how them affects the average length and number of microtubules. The simulation time acceleration is set to 5x real time. Each time a parameter is varied and others are controlled factors. The record is taken when the simulation has reached steady state and graphs are plotted.
Results
Part1 – How to achieving greatest average length of microtubules Fixed parameter | Shrink rate | Catastrope | Rescue
Release | MED | Nuc rate | Nuc sites | Variable Growth rate | 0.263 | 0.042 | 0.064
0.024 | 0.8 | 0.02 | 180 | Result | 1 | 2 | 3 | 4 | 5 | Mean | 0.14 | 32.9 | 21.12 | 23.93 | 23.95 | 27.54 | 25.888 | 0.16 | 33.19 | 36.82 | 32.5 | 28.83 | 30.15 | 32.298 | 0.18 | 29.79 | 39.11 | 41.19 | 40.8 | 31.54 | 36.486 | 0.2 | 40.77 | 41.19 | 45.94 | 38.28 | 47.66 | 42.768 | 0.22 | 38.66 | 47.49 | 48.53 | 48.55 | 47.96 | 46.238 | 0.24 | 42.25 | 45.31 | 45.25 | 46.81 | 40.95 | 44.114 |
Table1
Figure1 Fixed parameter | Growth rate | Shrink rate | Catastrop/
Release | MED | Nuc rate | Nuc cites | Variable
Introduction
Microtubules are important for maintaining cell structure, intracellular transport, formation of mitotic spindle, as well as other cellular processes. Investigation of dynamics of microtubule assembly and disassembly allow us to understand the malfunction of mitotic spindle formation or other cellular processes. This experiment is divided into two parts; we are going to find out the critical parameters for achieving greatest average length of microtubules in part one and achieving the greatest number of microtubules in part two.
Principle
In this experiment, we used a simulation programme to explore how various factors change the way microtubules grow out from centrosome, and the shrink back. Growth rate, shrink rate, catastrophe rate, rescue rate, release rate, minus end end depolymerization rate, nucleation rate and nucleation site are the factors we can adjust to see how them affects the average length and number of microtubules. The simulation time acceleration is set to 5x real time. Each time a parameter is varied and others are controlled factors. The record is taken when the simulation has reached steady state and graphs are plotted.
Results
Part1 – How to achieving greatest average length of microtubules Fixed parameter | Shrink rate | Catastrope | Rescue
Release | MED | Nuc rate | Nuc sites | Variable Growth rate | 0.263 | 0.042 | 0.064
0.024 | 0.8 | 0.02 | 180 | Result | 1 | 2 | 3 | 4 | 5 | Mean | 0.14 | 32.9 | 21.12 | 23.93 | 23.95 | 27.54 | 25.888 | 0.16 | 33.19 | 36.82 | 32.5 | 28.83 | 30.15 | 32.298 | 0.18 | 29.79 | 39.11 | 41.19 | 40.8 | 31.54 | 36.486 | 0.2 | 40.77 | 41.19 | 45.94 | 38.28 | 47.66 | 42.768 | 0.22 | 38.66 | 47.49 | 48.53 | 48.55 | 47.96 | 46.238 | 0.24 | 42.25 | 45.31 | 45.25 | 46.81 | 40.95 | 44.114 |
Table1
Figure1 Fixed parameter | Growth rate | Shrink rate | Catastrop/
Release | MED | Nuc rate | Nuc cites | Variable