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Different Water States and Locations in Pollen Grains

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Different Water States and Locations in Pollen Grains
Research Proposal
DIFFERENT WATER STATES AND LOCATIONS IN POLLEN GRAINS

• Principal investigator, visiting scientist

Ettore Pacini

Nationality: Italian Position: professor of Botany Place of work: Dipartimento di Biologia Ambientale, Sezione Botanica, Universitá di Siena, Via P.A. Mattioli 4, 53100 SIENA Italy. Phone –39 0577 232863; Fax –39 0577 232860; E-mail pacini@unisi.it

Partecipating researchers

* Massimo Nepi

Nationality: Italy Position: Laboratory technician Place of work: Dipartimento di Biologia Ambientale, Sezione Botanica, Universitá di Siena, Via P.A. Mattioli 4, 53100 SIENA Italy. Phone –39 0577 232867; Fax –39 0577 232860; E-mail nepim@unisi.it

* C.J. Keijzer

Place of work: Laboratory of Experimental Plant Cytology Arboretumlaan 4, 6703 BD WAGENINGEN The Netherlands * H. Van As Wageningen NMR Centre and Lab of Molecular Physics Dreijenlaan 3, 6703 HA WAGENINGEN The Netherlands • Requested support - Variable field machine (Maran). Period of measurements: 10 days - NMR imager. Period of measurements: 10 days • Length of fellowship one months

• Project description
1. Introduction
Mature pollen of different species are dehydrated to different degrees at dispersal. In most cases, they become very dehydrated, with a water content of 10-20% on average. Pollen of this type is usually small (longitudinal diameter 30-60 µm), prolate and tricolpate or tricolporate. Less frequently pollen are only slightly dehydrated with a water content above 50%. This type of pollen is usually large, spherical and uni- or polyporate. The mean life of a pollen grain is strongly influenced by its water content at dispersal: the lower the water content, the longer the mean life. Very dehydrated pollen usually has a high cytoplasmic mono-, oligo- and polysaccharide content and no starch, unlike only slightly dehydrated pollen which contains starch

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