BACHELOR’S FINAL PROJECT
Title: Purifying microbe derived therapeutics from fermentation broth
Department: Research and Development - Product Development and Processing, Downstream processing laboratory
Supervisor at Biocon: Dr. Nitin Patel, Head -Department of Downstream Processing, Biocon India Private Limited, Bangalore
College supervisor: Dr: T.Yasodha, Head- Department of Biotechnology, Shri Andal Alagar College of Engineering, Mamandur, Tamilnadu, India
Objective: Purifying microbe derived therapeutics from fermentation broth by various chromatography and analysis using RP HPLC techniques- specializations on lipstatin drug.
Abstract: The main focus area of this project is to purify the microbe derived drug lipstatin by using various trials of chromatographic columns with different packing materials or varying mobile phase composition or altering the concentration or volume of the load of the crude sample, which has to be purified. Till date I have done trials with silica gel columns. I propose in future to perform trials with Alumina, Hydrophobic interaction columns and Crystallization studies. I have also plans to cross check the assay purity of the fractions collected from the chromatographic runs. This is a very crucial project as it mainly aims in separating the impurities from the drug.
Results: Derived a product from the column, which has about 90 to 95 percent pure product as its assay. This must also take into consideration the cost of the technique involved. So I mainly focus on an economical way to purify the drug lipstatin
Techniques: Preparative HPLC, Analytical HPLC, Hydrophobic interaction chromatography, Normal phase chromatography, Reverse phase HPLC, Column packing, and Assay of samples, celite bed filtration.
Instruments using: Preparative HPLC, Analytical Reverse Phase HPLC, Rotovapor, Sonicator, and Software for obtaining chromatograms
Title: “PRODUCTION OF ZIRCONIUM