Preview

Compare And Contrast Nitrate And Nitrite In Drinking Water

Good Essays
Open Document
Open Document
1027 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Compare And Contrast Nitrate And Nitrite In Drinking Water
2.23 Nitrate-Nitrogen and Nitrite-Nitrogen in Drinking Water
Nitrate may be naturally occurring, although its presence in drinking water is more often associated with contamination by excessive use of fertilizers (both inorganic and organic), in addition to inappropriate farming practices and sewage. The high nitrate level in drinking water is also associated with the simultaneous presence of bacterial contamination (World Health Organisation, 1996).

The primary health hazard from drinking water with nitrate-nitrogen occurs when nitrate is transformed to nitrite in the digestive system. The nitrite oxidizes iron in the haemoglobin of the red blood cells to form methaemoglobin, which lacks the oxygen-carrying capacity of haemoglobin. This
…show more content…
It is one of the most common constituents present in natural waters ranging from zero to several hundred milligrams per litre depending on the source and treatment of the water (Putz, 2003). Calcium is largely responsible for water hardness, and may negatively influence toxicity of other compounds. Elements such as copper, lead and zinc are much more toxic in soft water. Calcium carbonate has a positive effect on lead water pipes, because it forms a protective lead (II) carbonate coating. This prevents lead from dissolving in drinking water, and thereby prevents it from entering the human body. Calcium phosphate is a supporting substance and it causes bone and tooth growth, together with vitamin D. Bones decalcify (osteoporosis) and fractures become more likely if a body is not getting enough calcium. Since a guideline value is proposed for total hardness, no guideline value is proposed for calcium concentration in drinking water (World Health Organisation, …show more content…
It also does not grow and reproduce in the environment. As a result, it is considered to be the species of coliform bacteria that is the best indicator of faecal pollution (Edberg et al., 2000). They also indicate the possible presence of pathogenic bacteria, viruses and protozoans (Kara et al., 2004). Almost all the methods used to detect total coliforms, faecal coliforms and Escherichia coli are enumeration methods that are based on lactose fermentation (American Public Health Association, 1992). The Most Probable Number (MPN) method is a statistical, multi-step assay consisting of presumptive, confirmed and completed phases. In the assay, serial dilutions of a sample are inoculated into broth media.

Analysts score the number of gas positive (fermentation of lactose) tubes, from which the other two phases of the assay are performed and then use the combinations of positive results to estimate the number of bacteria present. Typically, only the first two phases are performed in coliform and fecal coliform analysis, while all three phases are done for Escherichia coli (American Public Health Association,

You May Also Find These Documents Helpful

  • Good Essays

    Indole Test Lab Report

    • 1279 Words
    • 6 Pages

    pneumoniae does not produce proteases and cannot break down proteins via proteolysis. The fat hydrolysis test was performed to determine whether our bacteria produces lipase, an enzyme that breaks down fat. The fat hydrolysis test was positive, showing K. pneumoniae produces lipase and can break down fats. The indole test was performed to determine if our bacteria can break down tryptophan via the enzyme tryptophanase. Our indole test came back negative, meaning K. pneumoniae does not produce tryptophanase and does not break down tryptophan into indole, ammonia, and pyruvic acid. The urea test was performed to determine if urea is hydrolyzed via the enzyme urease. The urea test was positive, meaning K. pneumoniae produces urease to break down urea. An inoculation onto Kligler’s iron agar determines if an organism can ferment glucose and lactose, it also detects the production of hydrogen sulfide from the breakdown of cysteine. Our Kligler’s iron agar showed acid with gas production, meaning K. pneumoniae fermented both glucose and lactose. The hydrogen sulfide production was negative. A Litmus Milk test is done to determine whether the organism can ferment lactose, digest the milk proteins using proteases, cause the…

    • 1279 Words
    • 6 Pages
    Good Essays
  • Good Essays

    Bios242 Lab2

    • 987 Words
    • 4 Pages

    For this experiment we will be testing four different bacteria with four different tests, using glucose, lactose, and sucrose. Hopefully we will use the information from those test to be able to identify the organisms in each of the samples from the case studies. We will use the results from the four different tests along with the information of how different bacteria react to match up to the case scenario and identify the bacteria, then check to see if our guess was correct. The findings are that we were able to identify, by process of elimination, the four different test bacteria.…

    • 987 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Bio 205 Workshop 2

    • 3099 Words
    • 13 Pages

    4 Tests/ Parameters that are evaluated to classify bacteria are: procedure’s that determine an organism’s ability to ferment carious carbohydrates; utilize various substrates such as specific amino acids, starch, citrate, and gelatin; or produce waste products such as hydrogen sulfide gas and also differences in fatty acid composition of bacteria are also used to distinguish between bacteria.…

    • 3099 Words
    • 13 Pages
    Good Essays
  • Better Essays

    In this lab experiment we did several test to determine what our unknown bacteria was. To determine this we recorded the results of how the bacteria reacted to different media. Depending on the results of each test we could narrow down the different bacteria to determine what our unknown is. This experiment will also determine if our bacteria is a fermenter of sugars and if it is catalase positive. If the bacteria is a fermenter they will use the sugars to make ATP. If the bacteria is a fermenter of lactose/sucrose the EMB plate we used will “clearly differentiate between the colonies of lactose fermenting and non-fermenting microbes. In the same medium sucrose was also included to differentiate between coliforms that were able to ferment sucrose more rapidly than those that were unable to ferment sucrose” (Cheeptham & Lal, 2007).…

    • 1370 Words
    • 6 Pages
    Better Essays
  • Better Essays

    Unknown Lab Report

    • 2145 Words
    • 9 Pages

    The purpose of this lab was to identify two unknown bacteria cultures using various differential tests. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were oxidase, catalase, lactose and sucrose fermentation, Kugler/iron agar, nitrate reduction, gelatin hydrolysis, starch hydrolysis, manitol salt, MR-VP, citrate, bile esculin, indole, urease, DNase, and coagulase.…

    • 2145 Words
    • 9 Pages
    Better Essays
  • Good Essays

    Unknown Lab Report

    • 891 Words
    • 4 Pages

    An unknown bacterium was handed out by Dr. Honer. The appropriate tests were prepared and applied. The first procedure that was done was the gram stain. Under a microscope, if the gram stain is purple, the bacterium is gram positive, if the stain is red, it is gram negative. The next test was the fermentation tests for glucose, sucrose and lactose. If an organism is able to ferment the carbohydrate, there will be acid produced, which will turn the broth yellow. Homofermentation shows that only one product was produced-acid. Other organisms will carry out heterofermentation, the presence of acid and gas. If the organism is unable to ferment the sugar in the tube, there will be no change in the orange/red color of the broth and will be considered alkaline. The following tests that I performed were tests for methyl red and vogues proskauer in SIM media. A positive methyl red test indicates a mixed acid fermentation and would turn the organism’s broth red, if no color presents, it is negative. A positive vogues proskauer test indicates fermentation to neutral products and will turn the broth red, if no color presents, it is also negative. Stab inoculating the SIM media for these tests will also show if the organism produces sulfur or indole and if it is motile. If sulfur is present, then the medium will be black, if it produces indole, the medium will be red, and if it is motile, there will be growth throughout the tube. I also grew the organism on MacConkey agar. After inoculation, visible colonies may or may not be present.…

    • 891 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Unknown Lab Report

    • 1149 Words
    • 5 Pages

    To identify an unknown bacterial specimen using basic laboratory technique and biochemical tests. The unknown bacteria will be one of the following: Enterococcus faecalis, Staphylococcus saprophyticus, Escherichia coli, Enterobacter aerogenes, Proteus vulgaris, Salmonella [I assume typhimurium], or Shigella [either flexneri or sonnei, we used both in our lab during the semester].…

    • 1149 Words
    • 5 Pages
    Good Essays
  • Good Essays

    Unknown Bacteria Report

    • 586 Words
    • 3 Pages

    An unknown bacterium experiment, this experiment was done to discover what bacterium was in a mixed culture sample. This experiment worked with enteric bacteria which are members of the Enterobacteriaceae and live in the intestinal tract (Willey et al., 2014). Also found in Willey et al (2014) is some characteristics of enterics such as they all degrade sugars by means of the Embden-Meyerhof pathway, they produce plasmid-encoded proteins and that some of them perform mixed acid fermentation, some use enzyme systems like formate dehydrogenase and pyruvate formate-lyase (PFL) The first thing that had to be done was the streak plating to isolate the bacterium, then the next lab multiple tests were ran to help identify which bacterium was in the sample. One of the most important tests done was gram staining because it helped to limit the possible bacterium that it could have been. To be sure which bacterium was worked with we referred to the Bergey’s manual and compared the results to the possible bacterium.…

    • 586 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    Stream Ecology Intro

    • 1520 Words
    • 7 Pages

    Bacteria and toxic chemicals are a major factor, but they are not a helpful factor. Toxic chemicals in the stream are mainly caused by human activity. When toxic chemicals are present in the stream this could cause many problems and result in an unhealthy stream. Toxic chemicals such as the cancer-causing…

    • 1520 Words
    • 7 Pages
    Powerful Essays
  • Good Essays

    Nitrate Results Essay

    • 435 Words
    • 2 Pages

    The results for nitrate were inconclusive, there are traces of nitrate in wells 15, 20, 23, and 21 but we couldn’t find the source. We believe that number 15 is the source but there is very little amount of pollutants in the water to make the solution turn black. Wells 15, 20, 23, and 21 when tested for nitrates, the iodine changed to a yellowish blackish color. The hypothesis was not supported, well number 26 did not test positive for any of the pollutants. The first well tested was well number 15 and we believe that we found nitrate around the cattle farm because animal wastes contribute to nitrate contamination of ground water (bfhd.wa.gov).…

    • 435 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Proposal on Nitrates

    • 903 Words
    • 4 Pages

    A. Since 1971 nitrate concentrations in the Suwannee River have been increasing at a rate of 0.02 mg/L per year. The rise of nitrate concentrations found in the Suwannee River basin must be addressed. This problem is of extreme significance not only to hydrogeologists, but also anyone who cares about preserving natural Florida. Attempts to remove nitrate concentrations have been unsuccessful and limiting the amount used in agriculture will take years to improve the changed groundwater chemistry.…

    • 903 Words
    • 4 Pages
    Good Essays
  • Satisfactory Essays

    The secondary treatment consists of putting in microorganisms to consume the dissolved waste. The advanced treatment consists of filtrating and disinfecting the water. In between each of these three steps, we conducted several tests to test for key factors that determined whether or not the water is clean. We tested for pH, ammonia concentration, turbidity, and COD. Turbidity measured suspended particles in the water and the clarity of the water. COD stands for chemical oxygen demand and measures the amount of dissolved oxygen that would be removed from the water to react with pollution. In our end results, the water passed all four-test regulations and was indeed clean water. The pH of clean water should be between 6.0 and 9.0 and ours was 6.17. The ammonia concentration was no limit, but our concentration was very low at .18 mg/L. The turbidity levels in clean water should be less than 4 FAU and ours was 0.39 FAU. The COD levels…

    • 608 Words
    • 3 Pages
    Satisfactory Essays
  • Better Essays

    There are many problems having to deal with our water throughout the state of Iowa. In the articles, Iowa’s Nasty Water War, Conflict Over Soil and Water Quality Puts ‘Iowa Nice’ to a Test, and Dead Zones, all share important reasons to what is happening. A huge problem that is dealing with our water is nitrates, which is a serious acid and is making our water very unhealthy. All of these articles each share important reasons to why nitrates are so bad and the effect it has on people. Some problems are, that nitrates are costing cities way too much money, the struggle it puts on farmers, and the fact it is one of the causes to eutrophication.…

    • 621 Words
    • 3 Pages
    Better Essays
  • Powerful Essays

    This study aimed to determine the permanent water hardness in terms of calcium carbonate concentration of water samples from the Boys’ dormitory, Administration Building, Gymnasium, Girls’ dormitory, and Canteen of PSHS-IRC together with the commercial drinking water Nature Spring, Absolute, and Wilkins through indirect titration. Water hardness was identified by the amount of calcium carbonate (CaCO3) in ppm. The control group consisting of the branded commercialized drinking water had a concentration less than 43 ppm of CaCO3 and was considered as soft based on table of water hardness classification. The water samples collected from the Boys’ dormitory, Administration Building, Gymnasium, Girls’ dormitory, and Canteen have CaCO3 concentrations greater than 43 ppm and were classified as slightly hard. The water sample from the Boys’ dormitory had the highest concentration of 87.420 ppm and the water sample from Gymnasium had the lowest concentration of 52.712 ppm. Based from the results, the samples from the different sources contained significant amount of CaCO3 which may cause further problems in pipe clogging, health, laundry, and utility from these sources.…

    • 4755 Words
    • 20 Pages
    Powerful Essays
  • Powerful Essays

    Water Treatment Plants

    • 4417 Words
    • 18 Pages

    Surface waters necessitate water treatment before consumption to guarantee no health risk is present to the user. Poor quality water which consists of dissolved and suspended particles, can impact and cause health risks to consumers. The most important contamination is the microbiological contamination as it leads to infectious diseases (Water Treatment, 1994). Chemicals such as nitrates and cyanide when contaminate the water cause long-term health risk such as cancer, kidney and liver damages. Physical contamination may also cause a health risk such as eczema as it extends microbial survival (Drinking Water Treatment Chemicals, 2001).…

    • 4417 Words
    • 18 Pages
    Powerful Essays