Bacterial Growth Lab Paper
Study of Bacterial Growth and Resistance Level to Certain Antibiotics
INTRODUCTION
Escherichia coli—better known as E. coli—is a gram negative, rod shaped bacteria. It is relatively harmless, but can occasionally cause food poisoning. It can also provide Vitamin K2. It prevents the establishment of pathogenic bacteria, and is associated with or found in the intestinal organ. The antibiotic that E. coli is resistant to is Penicillin. Bacillus subtilis—better known as B. subtilis—is known as the hay bacillus or the grass bacillus. It is gram and catalase positive. It is rod-shaped, with the ability to form a tough, protective, shield around itself. It can tolerate extreme environmental conditions, and is an obligate aerob. The antibiotic that B. subtilis is resistant to is Tetracycline. When a bacteria is resistant to an antibiotic, it means that the antibiotic will have no effect in treating the bacteria. For example, E. coli is resistant to Penicillin, which means that if you were to try and treat E. coli with Penicillin, the treatment would fail and the E. coli bacteria would continue to grow. But, if you were to treat E. coli with Tetracycline, the antibiotic it is NOT resistant to, the E. coli would not be able to put up a fight, and would stop its growth. HA- If we add E. coli and B. subtilis to agar, and add Penicillin and Tetracycline to the agar, then the E. coli will grow more around the Penicillin and the B. subtilis will grow more around the Tetracycline, because E. coli is resistant to Penicillin and B. subtilis is resistant to Tetracycline. HO- If we add E. coli and B. subtilis to agar, and add Penicillin and Tetracycline to the agar, then the E. coli will grow more around the Tetracycline and the B. subtilis will grow more around the Penicillin, because E. coli is resistant to Tetracycline and B. subtilis is resistant to Penicillin.
MATERIALS (PER PAIR OF STUDENTS)
• 2 agar plates with nutrient sugar
• 2 disks of Penicillin
INTRODUCTION
Escherichia coli—better known as E. coli—is a gram negative, rod shaped bacteria. It is relatively harmless, but can occasionally cause food poisoning. It can also provide Vitamin K2. It prevents the establishment of pathogenic bacteria, and is associated with or found in the intestinal organ. The antibiotic that E. coli is resistant to is Penicillin. Bacillus subtilis—better known as B. subtilis—is known as the hay bacillus or the grass bacillus. It is gram and catalase positive. It is rod-shaped, with the ability to form a tough, protective, shield around itself. It can tolerate extreme environmental conditions, and is an obligate aerob. The antibiotic that B. subtilis is resistant to is Tetracycline. When a bacteria is resistant to an antibiotic, it means that the antibiotic will have no effect in treating the bacteria. For example, E. coli is resistant to Penicillin, which means that if you were to try and treat E. coli with Penicillin, the treatment would fail and the E. coli bacteria would continue to grow. But, if you were to treat E. coli with Tetracycline, the antibiotic it is NOT resistant to, the E. coli would not be able to put up a fight, and would stop its growth. HA- If we add E. coli and B. subtilis to agar, and add Penicillin and Tetracycline to the agar, then the E. coli will grow more around the Penicillin and the B. subtilis will grow more around the Tetracycline, because E. coli is resistant to Penicillin and B. subtilis is resistant to Tetracycline. HO- If we add E. coli and B. subtilis to agar, and add Penicillin and Tetracycline to the agar, then the E. coli will grow more around the Tetracycline and the B. subtilis will grow more around the Penicillin, because E. coli is resistant to Tetracycline and B. subtilis is resistant to Penicillin.
MATERIALS (PER PAIR OF STUDENTS)
• 2 agar plates with nutrient sugar
• 2 disks of Penicillin
References: Bernstein0275. (2013, April 22). Bacillus subtilis. Wikipedia. Retrieved from http://en.wikipedia.org/wiki/Bacillus_subtilis Lgnatzmice. (2013, September 05). Escherichia coli. Wikipedia. Retrieved from http://en.wikipedia.org/wiki/Escherichia_coli