The focus of this experiment was to identify unknown bacteria provided. The identification of unknown bacteria is beneficial in many aspects of the research of microorganisms and also aids physicians to treat patients correctly. Various tests were performed to provide the fermentation abilities, presence of particular enzymes, and biochemical reactions. Qualitative observations were made on the tests in identification process. The data from experimentation provided sufficient results to suggest that the identity of unknown bacterium was the gram-positive spore forming bacteria, Bacillus Subtilis.
One of the main responsibilities of a medical lab is to determine the identity of pathogenic bacteria. It is important to determine the specific type of bacterium causing disease so the physician is able to correctly treat the patients. The structure of bacteria plays a crucial role of what antibiotics works and which do not. The chemical reaction of the bacteria is also important. Most antibiotics alter or inhibit protein structure, inhibit transcription, inhibit translation, affect cell membrane structure, or alter cell-wall synthesis (1). Bacterial resistance is another pertinent medical reason for identification. The evolution of bacterial resistance has made treatment of diseases much more difficult. Therefore, it is important to know the chemistry, structure, and resistance of the pathogenic bacteria.
Bacillus subtilis, known also as the hay bacillus or grass bacillus, is a Gram-positive, catalase-positive bacterium (1). A member of the genus Bacillus, B. subtilis is rod-shaped, and has the ability to form a tough, protective endospore, allowing the organism to tolerate extreme environmental conditions (2). Although this species is commonly found in soil, more evidence suggests that B. subtilis is a normal gut commensal in humans. As a model organism B. subtilis is commonly used in laboratory studies directed at discovering