Bio 156 Lab
LAB 1 1. Describe the function of the following pieces of safety equipment and how each might be used: (10 points) a. Eye Wash (2 points)
In the event of a chemical splash to the eyes, flood the eyes for at least 15-30 minutes with water. Flush from the eye outward. If you were not wearing proper eye wear, a chemical could splash into your eye and would make it necessary to use this. b. Fire Blanket (2 points)
Used in order to put out a small fire. If a small area of the lab caught fire, a fire blanket could be used to put it out. c. Shower (2 points)
Safety showers are designed to flood your body with water in the event of a fire or chemical spill. Stand under and activate the shower for at least 15-30 minutes. If a chemical were to spill on a large portion of your body, the shower could be used to wash it off. d. Chemical Spill Kit (2 points)
Chemical spills are treated differently from biological spills. A bleach solution is not appropriate for a chemical spill. If the spilled chemical is an acid, then an appropriate base will be used to neutralize that acid. If the spill is a solution that is basic in nature, then an appropriate acid is used to neutralize the spill. (An acid + a base → a salt + water) Note that the product is neutral. A chemical spill kit would be used anytime a chemical is spilled in the lab. e. Biological Spill Solution (2 points)
A 10% bleach solution. If blood were being used in an experiment, to say look under a microscope and was accidently spilled, this solution would need to be used to clean it up. 2. Briefly describe a situation when each of the following waste disposal containers would be used and give examples of the waste involved: (4 points) a. Broken Glass Container (2 points)
A 50 ml beaker is being used to measure out a substance for an experiment and is dropped by a student. The glass from the beaker should be disposed of in the broken glass container. b. Biological Hazards Materials
In the event of a chemical splash to the eyes, flood the eyes for at least 15-30 minutes with water. Flush from the eye outward. If you were not wearing proper eye wear, a chemical could splash into your eye and would make it necessary to use this. b. Fire Blanket (2 points)
Used in order to put out a small fire. If a small area of the lab caught fire, a fire blanket could be used to put it out. c. Shower (2 points)
Safety showers are designed to flood your body with water in the event of a fire or chemical spill. Stand under and activate the shower for at least 15-30 minutes. If a chemical were to spill on a large portion of your body, the shower could be used to wash it off. d. Chemical Spill Kit (2 points)
Chemical spills are treated differently from biological spills. A bleach solution is not appropriate for a chemical spill. If the spilled chemical is an acid, then an appropriate base will be used to neutralize that acid. If the spill is a solution that is basic in nature, then an appropriate acid is used to neutralize the spill. (An acid + a base → a salt + water) Note that the product is neutral. A chemical spill kit would be used anytime a chemical is spilled in the lab. e. Biological Spill Solution (2 points)
A 10% bleach solution. If blood were being used in an experiment, to say look under a microscope and was accidently spilled, this solution would need to be used to clean it up. 2. Briefly describe a situation when each of the following waste disposal containers would be used and give examples of the waste involved: (4 points) a. Broken Glass Container (2 points)
A 50 ml beaker is being used to measure out a substance for an experiment and is dropped by a student. The glass from the beaker should be disposed of in the broken glass container. b. Biological Hazards Materials