Western Blotting
Western Blotting is used to Identify a Subunit of Myosin
Light Chain in the Proteins of All the Different Fish.
Chae Yee
Kent State University:
BSCI 30140 Lab
Abstract Western Blotting can be used to detect the Myosin actin light chain in different species of fish and is used to distinguish from different species based on variation, commonality, or evolutionary divergence. First, proteins are extracted from the tissue and loaded into a gel matrix. The matrix will separate the proteins according to size using an electric current. Proteins that are separated after are blotted from the gel and onto a paper membrane. An antibody is then added to the membrane paper and causes a colored reaction. Following the reaction, the results help detect and quantify a single protein among hundreds of other proteins in the sample. Western blotting is used during this procedure to test that proteins can be indicators of genetic and evolutionary similarity.
Results show that the different species of fish contain the myosin light chains that are equivalent in molecular mass, which then means they are similar in their evolutionary relationships.
Western Blotting is used to Identify a Subunit of Myosin Light Chain in the Proteins of All the Different Fish.
Introduction
Western blotting is a technique in biological research that allows scientists to identify and quantify specific proteins among a protein mixture. The method that is used is a “protein mixture is applied to gel-electrophoresis in a carrier matrix (SDS-PAGE) to separate a protein by size and charge. Next, the separated protein-bands are transferred into a carrier membrane. The proteins are then accessible for anti-bonding in order to detect them” (Antibodies-online.com, 2012). Myosin is a muscle protein that is essential to animals for survival and has remained stable over time. The myosin light chain can be compared from different species for evolutionary divergence and similarities. The objective of this
Light Chain in the Proteins of All the Different Fish.
Chae Yee
Kent State University:
BSCI 30140 Lab
Abstract Western Blotting can be used to detect the Myosin actin light chain in different species of fish and is used to distinguish from different species based on variation, commonality, or evolutionary divergence. First, proteins are extracted from the tissue and loaded into a gel matrix. The matrix will separate the proteins according to size using an electric current. Proteins that are separated after are blotted from the gel and onto a paper membrane. An antibody is then added to the membrane paper and causes a colored reaction. Following the reaction, the results help detect and quantify a single protein among hundreds of other proteins in the sample. Western blotting is used during this procedure to test that proteins can be indicators of genetic and evolutionary similarity.
Results show that the different species of fish contain the myosin light chains that are equivalent in molecular mass, which then means they are similar in their evolutionary relationships.
Western Blotting is used to Identify a Subunit of Myosin Light Chain in the Proteins of All the Different Fish.
Introduction
Western blotting is a technique in biological research that allows scientists to identify and quantify specific proteins among a protein mixture. The method that is used is a “protein mixture is applied to gel-electrophoresis in a carrier matrix (SDS-PAGE) to separate a protein by size and charge. Next, the separated protein-bands are transferred into a carrier membrane. The proteins are then accessible for anti-bonding in order to detect them” (Antibodies-online.com, 2012). Myosin is a muscle protein that is essential to animals for survival and has remained stable over time. The myosin light chain can be compared from different species for evolutionary divergence and similarities. The objective of this
References: Antibodies-online.com. (2012). Western Blotting: Background Information. Atlanta, GA. http://www.antibodies-online.com/resources/17/622/Western+Blotting+Background+information/ Cell Signaling Technology. (2012). Myosin Light Chain. Danvers, MA. Department of Biological Sciences. (2012). The Western Blot. Cell Biology Laboratory Manual. Kent State University. pp. 118-171