Preview

Science Investigatory Project (Chemistry)

Better Essays
Open Document
Open Document
1898 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Science Investigatory Project (Chemistry)
Guimaras State College Laboratory High School

Buenavista, Guimaras

A Science Investigatory Project:

Comparison of the Rate of Fermentation of Mangifera indica, Annona muricata, and Citrofortunella microcarpa juices

Presented to:

Mr. Ronelo Camarista

Science Instructor

-------------------------------------------­

Jeth Gallenero

Claire Sabilala

Gregleen Ga

Rona Marie Zaragoza

Geraldine Wilson

Charm Gaylan

Olive Rose Poticar

Comparison of the Rate of Fermentation of Mangifera indica, Annona muricata, and Citrofortunella microcarpa juices

ABSTRACT

This study aims to find out which among the juices of Mangifera indica, Annona muricata, and Citrofortunella microcarpa fruits ferment fastest. Three treatments were made: 200 mL. of Mangifera indica fruit juice fermented with 20 yeast cells; 200 mL. of Annona muricata fruit juice fermented with 20 yeast cells; and 200 mL. of Citrofortunella microcarpa fruit juice fermented with 20 yeast cells.

The start date of fermentation was recorded as well as the original specific gravity of each treatments. The end of fermentation of each treatment were watched for by the researchers. To verify a treatment ended fermentation, apparent attenuation was computed, computing prior to that the final gravity of each treatment.

The result showed that the treated Citrofortunella microcarpa fruit juice fermented fastest. Next was the treated Annona muricata fruit juice. The last to complete fermentation was the treated Mangifera indica.

This study is economically beneficial to the province, since it is abundant with the fruits used. It would also encourage small and medium enterprises to venture into liquor-making, even via home-based production.

The researchers recommend a similar research on other fruits such as cashew, jackfruit and bananas. Also recommended is the use of variables such as temperature, pressure and amount of fermentors added.

I. Introduction

A. Fermentation Fermentation, also



References: Katarzyna Chojnacka. Chemical Engineering and Chemical Process Technology, Volume V., Encyclopedia of Life Support Systems, UNESCO, 2008. Shurtleff, W. and Aoyagi, A. A Brief History of Fermentation, East and West, Soyinfo Center, 2007. Daniel I.C. Wang. Fermentation and Enzyme Technology, Wiley, 1979. Rene J. Dubos. "Louis Pasteur: Free Lance of Science, Gollancz.” Trends in Biotechnology, Volume 1, Cell Press, 1951. Merriam-Webster Collegiate Dictionary, 11th Ed, Merriam-Webster, Inc, 2010. Guimaras Quickstat, National Statistics Coordinating Board, 2007. http://www.nobelprize.org/nobel_prizes/chemistry/laureates/1907/buchner-bio.html, November 2, 2012. http://www.nobelprize.org/nobel_prizes/chemistry/laureates/1929/press.html, November 2, 2012. http://www.beer100.com/history/winehistory.htm, November 26, 2012. http://en.wikipedia.org/wiki/Cider, October 12, 2012.

You May Also Find These Documents Helpful

  • Better Essays

    Purpose: The purpose of the this experiment is to learn to transfer microorganisms to a nutrient medium to promote growth using aseptic techniques. which are a method used to prevent unwanted organisms from contaminating a medium. We will also learn about different types of culture media, including MRS broth and nutrient broth, learn about oxygen and temperature requirements for microbial growth and how to control microbial growth.…

    • 1308 Words
    • 6 Pages
    Better Essays
  • Better Essays

    Apple sauce lab

    • 700 Words
    • 7 Pages

    hypothesis: If we mix pectinase and cellulase then we will make the most applejuice , because the combination of the two chemicals will cause reactions and will yeild more juice than one enzyme.…

    • 700 Words
    • 7 Pages
    Better Essays
  • Good Essays

    Bio Lab

    • 573 Words
    • 3 Pages

    The lab is designed to investigate the effect of different enzymes on apple juice production…

    • 573 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    In the beginning, three fermentation vial were prepared with distilled water, yeast and eleven grams of glucose. A control vial was also prepared, but only contained water and yeast. Then all the fermentation vials were placed in a larger vial, and inverted. The CO2 was measured and then the vials were placed in designated location that contain different temperature. One vial was placed in the incubator and another in the ice bath. The control and room temperature vial were left in the table. Every five minutes for five interval the measurements of Co2 was collected from each vial.…

    • 98 Words
    • 1 Page
    Satisfactory Essays
  • Satisfactory Essays

    In this experiment, the amount of CO2 evolved for different concentrations of Convollaria roots was studied. The question being answered was what kind of effect the increase in concentration of Convollaria roots had on the rate of fermentation. The hypothesis was that the antimicrobial activities in Convallaria majalis’s roots would hinder the process of yeast fermentation because it will cause self- destruction or death in the microorganism of the yeast. If the antimicrobial activities in Convallaria majalis’s inhibit yeast fermentation, then the amount of CO2 evolved in the fermentation process will slowly decease or no CO2 will be formed. However, the results did not confirm our prediction because the Convallaria produced an increasing amount of CO2. Thus, the plants extract increased the rate of yeast fermentation.…

    • 351 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Cellular Respiration Lab

    • 452 Words
    • 2 Pages

    The two types of fermentation that are well known are alcoholic fermentation as well as lactic acid fermentation. Fermentation is vital for many organisms, such as yeast and bacteria, because it allows them to obtain energy required to carry on life processes. Alcoholic fermentation is especially important for human beings, as it is used to produce alcoholic beverages, bread, and many other everyday items that are consumed (Alba-Lois, 2010). On the other hand, lactic acid is a waste product of certain bacteria (Lactobacillales), which is utilized to create many dairy products such as yogurt and cheese. In addition, humans can resort to lactic acid fermentation when oxygen is limited, so it is used as an extra source to obtain oxygen. In our experiment we will be using yeast, a single-celled organism that utilizes sugar as a food source, and it produces energy substances through the breakdown of sugar molecules. Specifically, the type of sugar as a source of food, impacts the speed of fermentation in yeast. In this lab, we will calculate the rate of fermentation in yeast with different solutions of sugar, such as sucrose, fructose, and lactose with glucose being the control. It is important to humans that the yeast uses the best sugar source during fermentation, as it creates important everyday items we consume like bread, alcohol, and…

    • 452 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Some bacteria ferment certain carbohydrates, while producing acidic or gaseous products; this information can be utilized in order to identify bacteria (Morello and Granato, 2003). In order to test for carbohydrate fermentation, tubed broths, containing a Durham tube for trapping any gas formed when the broth is incubated, are utilized (Morello and Granato, 2003). After incubation, a color change indicates that an acid product was formed, and a bubble in the Durham tube indicates that a gas was formed. In this study, carbohydrate fermentation tests for the presence of glucose, maltose, lactose, and sucrose in Proteus Vulgaris were performed. The results were then compared to the results of prior experiments.…

    • 302 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Fermentation Mic

    • 1474 Words
    • 6 Pages

    Using Selective Media to Monitor the Fermentation Process of Cabbage and Cucumber, for Fourteen Days…

    • 1474 Words
    • 6 Pages
    Good Essays
  • Powerful Essays

    The bile esculin hydrolysis test is a selective and differential slant used to identify bacteria of the genus Enterococcus. The test contains bile salts to select for the desired bacteria, and differentiates because the hydrolysis of esculin and subsequent combination of the products with iron produces a black color. A positive test for the bile esculin slant is a completely blackened tube (Lindell et al, 1975). A triple sugar iron (TSI) slant is used to identify sugar fermentation in a microorganism; it contains a red pH-sensitive dye that will turn yellow under acidic conditions, such as contact with the acidic byproducts of sugar fermentation. The three sugars, sucrose, lactose and glucose, are present in specific concentrations, 1%, 1%, and .1% respectively (Hajna, 1945). The combination of the color change results and the location in the tube of the changes allows for a multitude of varying results. The TSI slant is a useful launch point for an investigation of this type, because the varying results can give a solid idea of what direction the remainder of the tests must take. A citrate utilization test is used to determine if an organism uses citrate as its only source of carbon, a positive result will change the dye in the slant from green to blue due to the byproducts changing the pH in the tube (Kiska…

    • 2799 Words
    • 12 Pages
    Powerful Essays
  • Good Essays

    Introduction: The fermentation of foods has been a long standing tradition and practice among many civilizations, as it was once a means to keep foods fresh and or edible over long periods of time. Today the process of fermentation has become a living/industry for some, and a hobby for others. For the purposes of this lab it was asserted that there will be no use of vinegar (ascetic acid), and instead merely distilled…

    • 842 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Investigating the Effect of Enzyme Concentration on Pectinase in the Production of Fruit Juice In this practical, I shall demonstrate the relationship between the concentration of pectinase and the amount of fruit juice produced accordingly in proportion. Apparatus: Apples Pectinase solution 10 x filter paper Knife or kitchen mincer Glass rod Water bath X 1 250 ml beakers X 2 Wash bottle of distilled water 100 cm^3 measuring cylinders In the case of the pectinase, the concentration had to be divided into sufficient amounts from which I can gain sufficiently accurate results to picture any relations between the concentration of the enzyme solution and the apple juice produced.…

    • 1140 Words
    • 5 Pages
    Good Essays
  • Powerful Essays

    • [Brady Burkhart, Terrell Grayson and Eric Kimler, 2009. Yeast Fermentation Optimization Project#4 [pdf]. 17th September 2009. Available at http://unitopslab.okstate.edu/yeast%20fall%202%2009.pdf [accessed 15:08, 11.11.2012]]…

    • 1723 Words
    • 7 Pages
    Powerful Essays
  • Good Essays

    In additional to the usual alcoholic fermentation, yogurt may undergo other changes caused by microorganisms:…

    • 546 Words
    • 3 Pages
    Good Essays
  • Good Essays

    2. as during the preparation of the experiment, some bacteria may stick on the apparatus so the fruit juices may be contaminated and the protein in the juices may also be digested by the bacteria thus affecting the result. Therefore, it is suggested that all apparatuses used should be well washed and heated with non-luminous Bunsen flame to kill the bacteria contained…

    • 1129 Words
    • 5 Pages
    Good Essays
  • Powerful Essays

    santol vinegar

    • 7121 Words
    • 25 Pages

    Kings and world leaders from the date of antiquity to modernity always come together to a ceremonial toast of wine as a symbol of friendship, cooperation and unity for their nations and people to live in peace, harmony and goodwill and thus liberate their kingdoms and states from the vestige of conflicts, enmities and wars. So that at the end of the day upon sipping a glass of wine, they say, “it is a living thing not only to be drunk but to be enjoyed.”…

    • 7121 Words
    • 25 Pages
    Powerful Essays