Lab 4 Diffusion and Osmosis
1. Diffusion and Osmosis, June 4, 2013
2. Purpose: The purpose of this lab is to help give visual understanding of diffusion which is a solution of high concentration spontaneously (no energy required) moving to an area of low concentration. Also taking a look at osmosis, which is the movement of chemicals across the cell membrane. Osmosis requires some type of energy to be put in for this to happen. After these experiments we should have a better idea and visualization of how chemicals transfer across the cell membrane.
3. Materials:
Dropper bottle of water
Compound microscope
Dissecting needle
Carmine powder
Slide and coverslip
3 test tubes
Transfer pipets
2 400 ml beaker
30 cm moist dialysis tubing
500ml beaker
Hot plate
Benedicts reagent
I2KI solution starch solution
Wax pencil
30% glucose solution
String or rubber band
Test tube rack
Slides and cover plates
4. Methods and Procedures:
Experiment 1:
Prepare a slide of dry carmine and water and cover slip
Put under the microscope, examine under the different magnifications, record you findings to later set up your conclusion.
Experiment 2:
Use dialysis tubing that has been soaking in water, fold it “accordion style” and use string to close the ends like a bag.
Roll opposite end of bag till it opens and add 4 pipettes of 30% glucose into it.
Add 4 pipettes of starch solution into it. Mix contents with bag closed. Then rinse with tap water.
Add 300 mL of water to a 400- to 500- ml. add a few drops of solution till it turns an amber-yellow color.
Leave bag in beaker for 30 minutes.
Remove bag then let it dry.
Record observations.
Experiment 3:
Obtain 7 equal size bore hole cylinder from the potato, cut roughly to the same size record the length and weight of each making sure they remain order.
Next is to collect 7 cups filled with 100 ml of the 6 different concentrations of glucose (, 0.1 0.2, 0.3, 0.4, 0.5, 0.6, and water).
Record the time and submerge the
2. Purpose: The purpose of this lab is to help give visual understanding of diffusion which is a solution of high concentration spontaneously (no energy required) moving to an area of low concentration. Also taking a look at osmosis, which is the movement of chemicals across the cell membrane. Osmosis requires some type of energy to be put in for this to happen. After these experiments we should have a better idea and visualization of how chemicals transfer across the cell membrane.
3. Materials:
Dropper bottle of water
Compound microscope
Dissecting needle
Carmine powder
Slide and coverslip
3 test tubes
Transfer pipets
2 400 ml beaker
30 cm moist dialysis tubing
500ml beaker
Hot plate
Benedicts reagent
I2KI solution starch solution
Wax pencil
30% glucose solution
String or rubber band
Test tube rack
Slides and cover plates
4. Methods and Procedures:
Experiment 1:
Prepare a slide of dry carmine and water and cover slip
Put under the microscope, examine under the different magnifications, record you findings to later set up your conclusion.
Experiment 2:
Use dialysis tubing that has been soaking in water, fold it “accordion style” and use string to close the ends like a bag.
Roll opposite end of bag till it opens and add 4 pipettes of 30% glucose into it.
Add 4 pipettes of starch solution into it. Mix contents with bag closed. Then rinse with tap water.
Add 300 mL of water to a 400- to 500- ml. add a few drops of solution till it turns an amber-yellow color.
Leave bag in beaker for 30 minutes.
Remove bag then let it dry.
Record observations.
Experiment 3:
Obtain 7 equal size bore hole cylinder from the potato, cut roughly to the same size record the length and weight of each making sure they remain order.
Next is to collect 7 cups filled with 100 ml of the 6 different concentrations of glucose (, 0.1 0.2, 0.3, 0.4, 0.5, 0.6, and water).
Record the time and submerge the