Preview

Lab 4 Diffusion and Osmosis

Good Essays
Open Document
Open Document
657 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Lab 4 Diffusion and Osmosis
1. Diffusion and Osmosis, June 4, 2013

2. Purpose: The purpose of this lab is to help give visual understanding of diffusion which is a solution of high concentration spontaneously (no energy required) moving to an area of low concentration. Also taking a look at osmosis, which is the movement of chemicals across the cell membrane. Osmosis requires some type of energy to be put in for this to happen. After these experiments we should have a better idea and visualization of how chemicals transfer across the cell membrane.

3. Materials:

Dropper bottle of water
Compound microscope
Dissecting needle
Carmine powder
Slide and coverslip
3 test tubes
Transfer pipets
2 400 ml beaker
30 cm moist dialysis tubing
500ml beaker
Hot plate
Benedicts reagent
I2KI solution starch solution
Wax pencil
30% glucose solution
String or rubber band
Test tube rack
Slides and cover plates

4. Methods and Procedures:
Experiment 1:
Prepare a slide of dry carmine and water and cover slip
Put under the microscope, examine under the different magnifications, record you findings to later set up your conclusion.
Experiment 2:
Use dialysis tubing that has been soaking in water, fold it “accordion style” and use string to close the ends like a bag.
Roll opposite end of bag till it opens and add 4 pipettes of 30% glucose into it.
Add 4 pipettes of starch solution into it. Mix contents with bag closed. Then rinse with tap water.
Add 300 mL of water to a 400- to 500- ml. add a few drops of solution till it turns an amber-yellow color.
Leave bag in beaker for 30 minutes.
Remove bag then let it dry.
Record observations.

Experiment 3:
Obtain 7 equal size bore hole cylinder from the potato, cut roughly to the same size record the length and weight of each making sure they remain order.
Next is to collect 7 cups filled with 100 ml of the 6 different concentrations of glucose (, 0.1 0.2, 0.3, 0.4, 0.5, 0.6, and water).
Record the time and submerge the

You May Also Find These Documents Helpful

  • Powerful Essays

    Diffusion and Osmosis

    • 1370 Words
    • 6 Pages

    Diffusion and osmosis are very important in biological process. Diffusion is the movement of molecules or ions from a region of their high concentration to a region of their low concentration. Osmosis is the diffusion of water molecules across a semipermeable membrane. To gain a better understanding of diffusion and osmosis, we examine multiple experiments to show diffusion and osmosis in animal cell, plant cell and synthetic cell. In our first experiment, we weight a decalcified chicken egg and place 10% NaCl concentration. At every fifteen minutes intervals, we weight the egg and record that the weight decreases. In our second experiment, we use three slices of aquatic plant Elodea and add pond water one leaf, distilled water for the second and 20% NaCl for the third, then observe the cells of each leaf with a compound microscope. Under microscope, chloroplasts (green organelles) in Elodea tend to bound to the cell wall in pond water, spread all over the cell in distilled water, pull away from cell wall in 20% NaCl. In our last experiment, we use a dialysis bag that contains 30% glucose and starch solution then place into a beaker of water and iodine solution. We then remove the bag out of the beaker, and use two test tube which label BAG, BEAKER to perform a Benedict’s test. We place solution in the bag in the BAG tube, and solution in the beaker in the BEAKER tube and add a drop of Benedict’s reagent to each tube, then heat each test tube and observe a color change in each tube. These experiments show that movement of water of chicken egg cell and Elodea cell is “osmosis” and movement of molecules in dialysis bag and beaker is “diffusion”.…

    • 1370 Words
    • 6 Pages
    Powerful Essays
  • Good Essays

    Osmosis And Diffusion Lab

    • 697 Words
    • 3 Pages

    Purpose: The purpose of this activity is to determine the effects of a selectively permeable membrane on diffusion and osmosis between two solutions separated by a membrane.…

    • 697 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    Diffusion of Osmosis

    • 786 Words
    • 4 Pages

    Materials used in this experiment were 6 strips of dialysis tubing, string, cups, distilled water, different concentrations of sucrose solutions.…

    • 786 Words
    • 4 Pages
    Powerful Essays
  • Powerful Essays

    Diffusion and Osmosis Lab

    • 1363 Words
    • 6 Pages

    1. To simulate and observe the diffusion of solutes and the osmosis of water through a semipermeable membrane through color change and sugar tests.…

    • 1363 Words
    • 6 Pages
    Powerful Essays
  • Better Essays

    Diffusion and Osmosis Lab

    • 1512 Words
    • 7 Pages

    Biology is the science of life and thus one of the many sciences that are part of everyday life. Diffusion and osmosis are processes that are a constant in our lives, even though many don’t realize it. Medicaments such as Fervex can be drunk only after diffusion has taken place and the powder granules have diffused into the hot cup of water. On the other hand, every day we become unintentional witnesses of osmosis when the roots of the plants try to suck up the water from the soil (1). The purpose of this lab is to show clearly in an understandable way how diffusion and osmosis happen and to help one realize the way they occur.…

    • 1512 Words
    • 7 Pages
    Better Essays
  • Satisfactory Essays

    We then used a cork borer to cut two holes in the agar 1cm apart. We then filled one hole with potassium ferricyanide. Next we filled the other hole with ferrous sulfate. We then observed the petri at an interval of 15 minutes. We observed and recorded results.…

    • 1828 Words
    • 8 Pages
    Satisfactory Essays
  • Good Essays

    To begin, this is my first time doing an experiment and a lab report for biology’s class. Although, it was a little hard at the beginning, my team and I got the hang of it and we finished it on time. It was actually kind of fun.…

    • 588 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Diffusion is movement of molecules from one area of concentration to another. This process is vital for the life functions of cells. Cells have selectively permeable membranes that allow only certain solutions to pass through them.…

    • 679 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    Osmosis Experiment

    • 1458 Words
    • 6 Pages

    Materials: Potato cylinders, 20 percent sodium chloride solution, three test-tubes, test-tube rack, scalpel blade, Gel ink pen, syringe-10cm^3, Plastic ruler with mm, tile, forceps filter paper, Masking tape.…

    • 1458 Words
    • 6 Pages
    Powerful Essays
  • Good Essays

    Osmosis Lab

    • 636 Words
    • 3 Pages

    Keep overnight to allow for osmosis to occur. 8. Remove each cube from the beaker, slightly dry it with a paper towel and weigh it, and record the results. Results The results in the graph show the average percentage change in mass of potato cells in comparison to the concentration of sucrose in the solution.…

    • 636 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    Diffusion is the movement of particles from areas of higher concentration to areas of lower concentration. It is a natural, random process. This means that it does not require extra energy input.…

    • 655 Words
    • 3 Pages
    Satisfactory Essays
  • Good Essays

    Biology Osmosis Experiment

    • 3078 Words
    • 13 Pages

    Cut a fresh baking potato into 5 mm slices. Cut four rectangles 2.5 cm x 1 cm from the slices as close to the same size as possible. Measure the length and width of each rectangle in metric units and place one in each of the containers, keeping track of which measured slice went in which container. There will be two slices (duplicates) for fresh water and two for salt water.…

    • 3078 Words
    • 13 Pages
    Good Essays
  • Better Essays

    Diffusion and Osmosis

    • 915 Words
    • 4 Pages

    Cut off a portion (around 1/5) of the potato, then scoop out a cavity from the other end. Stand the potato on its cut-off region inside the cup with water. Make sure that the water level is below the bottom of the…

    • 915 Words
    • 4 Pages
    Better Essays
  • Good Essays

    The effect of caffiene

    • 908 Words
    • 4 Pages

    3. Place the glass slide onto the stage of the microscope and observe the daphnia under the low light setting. Make sure you have correctly located the heart beating and not mistaking it for the movement of the gut or flapping of the legs.…

    • 908 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Osmosis and Diffusion

    • 563 Words
    • 2 Pages

    The first lab we did was our diffusion lab. We learned that diffusion is moving something from an area of high concentration to a low concentration. We were showed that Mio and other flavor drops perform diffusion when put in water as an example. For the lab part we put a mixture of starch and water into a plastic Ziploc bag and submerging it into a solution of water and iodine. The bag was semi-permeable and so some of the iodine leaked through into the bag showing osmosis. The iodine, being the indicator, changed the color of the starch mixture to have streaks of light gray with a purple tint almost. We also learned that molecules tend to move from areas of high concentration to areas of low concentration, also known as the Passive Transport.…

    • 563 Words
    • 2 Pages
    Good Essays