Blood Analysis: Computer Simulation
Blood is a combination of plasma (watery liquid) and red and white blood cells as well as platelets. It is a specialized bodily fluid that supplies essential substances and nutrients, such as sugar, oxygen, and hormones to our cells. Blood analysis can determine how functions of the body are working and if they are working properly. The purpose of this lab is to understand how various ailments, locations, and blood types can affect blood analysis.
Hypothesis- Activity 1: In higher demographic elevation hematocrit levels would be higher. Also, in blood samples with aplastic anemia and iron deficiency hematocrit levels would show to be lower than the average: 47% for males and 42% for women. Hypothesis- Activity 2: I anticipate that other than the healthy individual all remaining blood samples will have high sedimentation rate. Hypothesis- Activity 3: I would suspect that Hemoglobin levels would be much lower for the Iron deficient female and that the Polycythemia Male and Olympic female would have higher levels. Hypothesis- Activity 4: Blood types will be able to be detected through the presence of antigens
Materials and Methods
For this lab, we used these materials as listed below.
* PhysioEx 8.0 Software disk
* Collin College Human Anatomy and Physiology Lab manual
To conduct this lab, the following steps were taken:
You will need to open the Hemtocrit determination exercise within the PhysioEX program. 6 blood samples are given simulating a healthy male living in Boston, a Healthy female living in Boston, Healthy male living in Denver, a Healthy female living in Denver, as well as a Male with aplastic anemia and a female with iron-deficiency anemia. You then take blood from the simulated blood samples fill the Heparinized capillary tubes, seal it with the capillary sealer and place it in the microhemocrit centrifuge for five minutes and record the results.
You will need to open the Erthrocyte sedimentation rate exercise within the PhysioEX program. 6 blood samples are given simulating a healthy individual, menstruating female, person with sickle cell anemia, person with iron deficiency anemia, person suffering from myocardial infarction and person suffering from angina pectoris. Click the 6 blood samples and place them in the test tubes and add 3.8% sodium citrate. After mixing, move each solution in to the sedimentation tubes. Set the timer for 60 minutes and then measure the amount of blood that settled and record the results.
You will need to open the Hemoglobin (hb) determination exercise within the PhysioEX program. 5 blood samples are given simulating a healthy male, healthy female, female with iron-deficiency anemia, male with polycythemia, and a female Olympic athlete. Click on the blood samples and place samples onto a slides and stirring with a hemolysis stick for 45 seconds. You will then drag the slide to the hemoglobinometer and the left side will omit a green light showing the intensity of the blood sample. You will need to click the crank on the right side in order for the circle to match completely and record the data. Activity Four:
You will need to open the blood typing results exercise within the PhysioEX program. 6 blood samples are given simulating different blood types. You click on the blood samples placing them onto the slide with three wells, one place for each of the factors you are testing for: A, B and Rh. You then click on the corresponding anti-serum and add to the correct well on the slide (anti-A serum in A, anti-B serum in B and anti-Rh serum in Rh). Using the correct stick that corresponds, you mix each well and place under the light box to look for coagulation as positive results. Data is then recorded.
| Height of Column of blood
| Height of red...
Cited: Marieb, E. (2009). Human anatomy & physiology laboratory manual. (Custom ed.). New York: Pearson.
Physio-ex version 8:laboratory experiments in physiology [DVD]. (2008).
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