Preview

bio lab report

Good Essays
Open Document
Open Document
756 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
bio lab report
The Variation of Yeast Cells When Adding an Antibiotic
Biology 1401- A

October 21, 2013
Abstract. -- The main focus in this experiment was to find out if antibiotics affect yeast cells culture when added. The observation of the two cultures along with the yield of yeast cell reproduction between the two cultures was the sole purpose of the experiment; culture-A (- antibiotic) and culture-B (+ antibiotic). Our hypothesis stated whether antibiotic effects yeast cells cultures. This theory was tested by having a chamber with quadrants that contain the culture, a hemacytometer, was used to carry out this experiment. After the data was collected from using the hemacytometer, the results of the difference between the two cultures was to be determined through a t-test. As the test was conducted, the study shows that the difference between lacking an antibiotic and containing one is not different. An antibiotic in culture-A and culture-B does not affect the number of yielded yeast cells.
Introduction. -- It is undetermined whether or not antibiotics effect yeast cells reproduction. It has been hypothesized that antibiotics decrease the yield of yeast cells. This statement above is misinforming because antibiotics are used to kill bacteria, and yeast are not bacteria. Bacteria are prokaryotic while yeast, Saccharomyces cerevesiae, is eukaryotic. Yeast is used for fermentation to produce alcohol, and as well as to help give bread its shape (formation). Antibiotics have the same effect on both yeast and bacteria although they do not have the same structure. The experiment was carried out to compare the growth of cells in two different yeast cultures; culture-A lacked the antibiotic and culture-B contained the antibiotic.
Materials and Methods. -- To conduct this experiment there must be the following; a hemacytometer, a Pasteur pipette, and a compound microscope. A hemacytometer is a counting chamber to count the number of cells in a known volume. Pasteur pipettes

You May Also Find These Documents Helpful

  • Powerful Essays

    Gene Therapy: Lab Report

    • 2285 Words
    • 8 Pages

    Both hands and bench tops were sterilized by 10% ethyl alcohol and were continually wiped down at various times throughout the lab. Gloves were also worn for the duration of the lab to help prevent contamination. The first step was to obtain both strains of yeast, EAY 235 and EAY 431, with the fat end of a sterile tooth pick from an augur plate and place them into two separate Eppendorf…

    • 2285 Words
    • 8 Pages
    Powerful Essays
  • Good Essays

    Microbiology

    • 656 Words
    • 3 Pages

    Samples of S. cerevisiae and S. epidermidis were placed into sodium chloride (NaCl) solutions of 1, 7 and 15 percent which were supplied in the lab kit. The S. cerevisiae results are as follows. After 24 hours of observation there was a moderate amount of growth in the 1% NaCl solution which had a cloudy overall appearance and the growth appeared to be even throughout the specimen. There was minimal growth in the 7% NaCl solution and most of that growth was adhering to the sidewall of the tube. No growth was observed in the 15% NaCl solution. The results of the S. epidermidis specimens were very similar to those of S. cerevisiae. In the 1% NaCl solution a large amount of growth was noted. This specimen was not as cloudy as that of the S. cerevisiae but growth was consistent throughout the tube. Moderate growth was noted in the 7% NaCl…

    • 656 Words
    • 3 Pages
    Good Essays
  • Better Essays

    Soil Microbe Lab

    • 1259 Words
    • 6 Pages

    The objective of this soil microbe experiment is to isolate potential antibiotic-producing microbes in the soil that was taken from a local sample. To do so, two different samples of bacteria have been taken and placed onto agar plates (labelled A and B). As we allow each sample to grow, our objective will be to see what, if any, antibiotics develop and inhibit the growth of the bacteria. We predict that antibiotics will form on the agar plates, thus inhibiting the growth…

    • 1259 Words
    • 6 Pages
    Better Essays
  • Powerful Essays

    Yeast Lab Report

    • 1408 Words
    • 6 Pages

    5. The report should be based solely on yeast cell viability at different glucose concentrations.…

    • 1408 Words
    • 6 Pages
    Powerful Essays
  • Powerful Essays

    The problem that was investigated in our experiment was to see which antibiotic was more effective in treating infections caused by S. marcescens. The results of this experiment are important because it shows us the susceptibility of this bacterium to both tetracycline and chloramphenicol under a controlled environment.…

    • 1151 Words
    • 5 Pages
    Powerful Essays
  • Better Essays

    Through four rounds of this experiment, the strongest bacteria were continuously selected against the weaker ones hence resulted in the size of the zone of inhibition becoming smaller and smaller. For the control group, the size of the zone of inhibition from the beginning had been significantly smaller than that of the experimental group. Throughout the experiment, the zone of inhibition did decrease but just for a relatively small degree. This suggests that the experiment was valid in testing antibiotic resistance. Among the data set, there are some potential outliers. For an item to be considered a potential outlier in this experiment it has to be greater or less than three standard deviations from the mean diameter for each round. In the experimental group, the potential outlier for round 3 is 36 mm and those for round 4 are 25 mm, 27 mm and 27 mm. In the control groups, there is one potential outlier for round 1, which is 20 mm, one potential outlier for round 3, which is 9 mm and two potential outliers for round four, which are 7 mm and 9 mm. The existence of these outliers may due to the experimenters’ not getting the exact bacteria from the edge of the zone of inhibition, closing the lid of the tube too tight so the E. Coli could not fully grow, or difference of the concentration of triclosin in some tubes. However, the number of these potential outliers is relatively insignificant to the…

    • 1422 Words
    • 6 Pages
    Better Essays
  • Better Essays

    The purpose of this paper was to inform the reader of the importance of microbiology in the world today. Microbiology studies the behavior of microorganisms and teaches students about the effect of bacteria, fungi, viruses, algae, and yeast on living things. Studying these microorganisms gives insight to the spread of different diseases and how they can be controlled, treated,…

    • 1765 Words
    • 8 Pages
    Better Essays
  • Good Essays

    However, some of the diseases started to build up a resistance to the antibiotics and in result, the antibiotics became ineffective. In order to help resolve the crisis, antibiotics were found through screenings of soil microorganisms. However, soil microorganism’s antibiotics were depleted by the 1960s and their antibiotic effects were unable to be replicated through synthesis. In this experiment, the researchers developed numerous methods to cultivate uncultured organisms in their environment. The goal of this experiment was to find antimicrobials in the uncultured soil. Through this successful experiment, the researchers were able to discover a new antibiotic…

    • 657 Words
    • 3 Pages
    Good Essays
  • Good Essays

    The purpose of this lab was to determine how yeast cells are affected by the concentration of a food source, and for our purposes, the food sources were corn syrup and molasses. Our hypothesis was that the yeast cells would ferment the most when there was a higher concentration of molasses/corn syrup.…

    • 623 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    First a control was established for E. coli in a 1.0x nutrient broth. This was…

    • 2818 Words
    • 12 Pages
    Powerful Essays
  • Powerful Essays

    Growth Curve Report

    • 1680 Words
    • 7 Pages

    In this experiment,the cell growth of yeast is measured by using spectrophotometer and hemocytometer.We learnt how specthophotometer and hemocytometer use and also we learnt qualifications of hemocytometer and spectrophotometer.Serial dilution was used for this experiment and it was very important.Because of the serial dilution,we measured the number of yeast cells. The graph of growth curve was drawn and bacterial life cycle was understood with the graph.The purpose of the experiment was to calculate and draw bacterial growth curve.…

    • 1680 Words
    • 7 Pages
    Powerful Essays
  • Good Essays

    Gram Negative

    • 1567 Words
    • 7 Pages

    Describe the appropriate specimen collection procedures, staining methods, and culture techniques used for isolation of yeast.…

    • 1567 Words
    • 7 Pages
    Good Essays
  • Powerful Essays

    Microbial metabolites used as drugs, especially the antibiotics and anti-neoplastic agents are produced by fermentation. For…

    • 11591 Words
    • 47 Pages
    Powerful Essays
  • Powerful Essays

    Development of bacterial resistance to the antimicrobial drugs identified the need to determine a given bacterium’s susceptibility or resistance to a given drug which prompted W. M. M. Kirby and A. W. Bauer to develop a single disk method for susceptibility testing. This experiment used the Kirby-Bauer disk diffusion test to measure the degree to which Penicillin, Streptomycin, Ampicillin, and Chloramphenicol inhibited the growth of the bacterium, Proteus vulgaris. The measured zone of resistance for each antibiotic was compared against antibiotic performance standards maintained by the Clinical Laboratory Standards Institute to determine whether Proteus vulgaris was susceptible, intermediate, or resistant to the antibiotics used in the experiment. Proteus vulgaris demonstrated susceptibility to Ampicillin, Streptomycin, and Chloramphenicol but resistance to Penicillin. The average zone of inhibition for Ampicillin measured in this experiment was just 2 mm above the threshold diameter for Proteus vulgaris to be classified as susceptible. Testing on a much broader scope and over an extended period of time would have to be undertaken to determine if Proteus vulgaris is strengthening in resistance to the antibiotics which are currently effective in treating infections caused by Proteus vulgaris.…

    • 3242 Words
    • 13 Pages
    Powerful Essays
  • Good Essays

    Ampicillin (antibacterial), Itraconazole (antifungal) as the standard drug at a concentration of 200µg/ml. LB Agar was used as the culture media for antibacterial and potassium dextrose agar was used as culture media for the antifungal activity. The results of the antimicrobial activity are shown in figures and tables.…

    • 1256 Words
    • 6 Pages
    Good Essays