Task 3: How could Thin Layer Chromatography (TLC) have been used to prepare a pure sample of aspirin? The method of using thin layer chromatography is easy‚ quick and relatively cheap to carry out this makes it ideal for preparing pure samples of aspirin. Equipment Required: • Thin Layer Chromatography Paper • Ruler • Pencil • Solvent • Beaker • Micro Capillary • Access to a fume cupboard • UV light • Clingfilm • Individuals sample of aspirin • Pure sample of aspirin • Sample of salicylic acid Method:
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it is possible to identify which peak correlates with which compound. Figure 2 contains a peak at around 500 and 700 nm which is a good indicator that that would-be chlorophyll b. This makes sense because this was the bottom band from the thin layer chromatography and chlorophyll b is the most polar compound. Chlorophyll B contains an aldehyde where chlorophyll A has a methyl group making it slightly more polar. Following that‚ figure 4 contains
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Column and Thin Layer Chromatography Beverly Abstract: Plant pigments were separated and concentrated from a crude spinach extract through the use of column chromatography and an eluatropic series of hexanes‚ hexane/acetone‚ and methanol. The pigments were analyzed using thin layer chromatography with a 30% ethyl acetate/hexane developing solvent. Introduction: Chromatography is a technique used to separate a mixture of two or more components based on
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COLUMN AND THIN LAYER CHROMATOGRAPHY Mark Paul P. Pastrana‚ Mariah Ericka M. Patawaran‚ Princess Juneire M. Peligro‚ Francisco Q. Pua III‚ Rose Anne L. Quyo and Janille P. Ragpa Group 8 2B Medical Technology Organic Chemistry Laboratory ABSTRACT The main objectives were to separate the colored components of malunggay leaves by means of column chromatography‚ as well as to determine the purity of the components using thin layer chromatography (TLC) and measure the Rf values of the colored
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Experiment 9 Thin Layer Chromatography Experiment: 9 Title: Thin Layer Chromatography Introduction: Chemical analysis is conducted on specific analytes (specific substance of interest in a mixture)‚ however it is often found that these analytes must be separated for the chemical analysis to conduct their analysis. Chromatography is a set up of laboratory a technique that is used to separate a chemical mixture. This technique is very useful as it allows us to follow the course of
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the lab is to separate the caffeine samples we are using‚ using thin layer chromatography. The solvent we are using for the separation is 3:1 mixture of Chloroform and Acetone. Principle: Thin layer chromatography (TLC) is an important technique that is useful for separating organic compounds. TLC is often used to monitor the progress of organic reactions and to check the purity of products. Separations in Thin layer chromatography involve distributing a mixture of two or more substances between
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liquid-liquid extraction. Then crude material obtained was then purified by sublimation. The isolated caffeine was subjected to thin layer chromatography (TLC) together with a standard caffeine. The Rf value obtained for both isolated caffeine and standard caffeine was 0.24. Keywords: solid-liquid extraction‚ multiple liquid-liquid extraction‚ sublimation‚ thin layer chromatography‚ percent caffeine Introduction Many organic compounds are obtained from natural sources through isolation. In this
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PAPER AND THIN-LAYER CHROMATOGRAPHY Lab 47 Michael Stravino‚ CHM 2211L‚ June 3‚ 2012 Abstract Thin-Layer Chromatography and Paper Chromatography can be used to identify identical compounds‚ unknown compounds‚ the number of components in a mixture‚ as well as other important uses not explored in this laboratory. This experiment explores these techniques to identify an unknown amino acid by TLC and analyze food colors using paper chromatography. By using 2.0 mL of dimethoxyethane and 18 mL of
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Experiment 10 Thin Layer Chromatography Purpose! To identify how many components are in a mixture and the identity of a compound in a mixture by comparing the Rf of a known compound with the Rf of an unknown compound. Intodection. In this experiment the thin-layer chromatography the (TLC) will be used to determine the composition of various over-the-counter analgesic. We will be given two commercially prepared TLC plates with a flexible backing and a silica-gel coating with a fluorescent
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Chromatography of Commercial Analgesics Chromatography of Commercial Analgesics CHEMISTRY 200L EXPT 4 PAGE 8 - 11 CHEMISTRY 200L EXPT 4 PAGE 8 - 11 Janna Vernice R. Villalon*‚ Christian V. Villanueva‚ Cyd Vincent L. Zamora Department of Chemistry‚ College of Science *Corresponding author; e-mail: janna.villalon@rocketmail.com Abstract In analyzing the chromatography of analgesics‚ thin layer chromatography (TLC) was used. A very thin (micron) film of silica is coated on a glass
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