The purpose of the lab “All in the Family” was to compare the reactivity of the halogens Chlorine‚ Bromine‚ and iodine by observing the reactions between their elemental forms and their ionic forms. To accomplish this experiment‚ we first added a squirt of pet ether to two test tubes. Then‚ we added the same amount of Cl2 to both of the tubes‚ shook the mixtures‚ and recorded the color that resided in each mixture as the initial color. After‚ a squirt of NaBr was added to one test tube‚ and a squirt
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Serratiopeptidase drug was purchased from MARS Therapeutics & Chemicals limited‚ Hyderabad‚ India. The standard drug Fenofibrate was purchased from USV limited‚ Himachal Pradesh‚ India. Assay kits for serum Total Cholesterol (TC)‚ Triglycerides (TGL)‚ High density lipoproteins were purchased from Erba Mannheim‚ Transasia Bio-Medicals limited‚ Himachal Pradesh‚ India. All other chemicals were of analytical grade. The experiment was conducted using [12] male Albino Wistar rats (150-200g)‚ at about
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moles Product yield: (0.000277 mol/0.00027 mol) x 100 = 102.5% yield *Note: A recording mistake was made in the lab notebook‚ where the final product was supposed to be 0.05g (calculations above) and not 0.55g (calculations below). 0.55g 9-fluorenone / (180.20g/mol) = 3.0 x 10-3 moles Product yield: (0.003mol/0.00027mol) x 100 = 1‚111% yield. 2. The Rf values were not recorded in lab. However‚ spot results were recorded. The TLC plates 1-3 showed the presence of starting material (SM) in the crude
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3.6. Conclusion Various solvent extracts from mahua flower and fruits showed varying degrees of antioxidant activity in different test systems in a dose-dependent manner. The antioxidant activity was correlated with the amount of total phenolics present in the respective extracts in each assay. 50% acetone proved to be the most efficient solvent for extraction of antioxidants from mahua as the related extract contained the highest amount of phenolic compounds and also exhibited the strongest antioxidant
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Experiment 9 Thin Layer Chromatography Experiment: 9 Title: Thin Layer Chromatography Introduction: Chemical analysis is conducted on specific analytes (specific substance of interest in a mixture)‚ however it is often found that these analytes must be separated for the chemical analysis to conduct their analysis. Chromatography is a set up of laboratory a technique that is used to separate a chemical mixture. This technique is very useful as it allows us to follow the course of
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Organic Chemistry Laboratory I Experiment #6 - Column and Thin-Layer Chromatography (TLC) of a Spinach Extract Pre-Lab Assignment (1) List 5 ways that TLC can be used in an organic chemistry lab experiment. To identify an unknown‚ to monitor the course of a reaction and assess the purity of its product‚ to determine the best solvent for a column chromatography separation‚ to determine the somposition of each fraction from a column chromatography separation‚ and to determine whether a substance purified
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the lab was to separate and analyse analgesic drugs in a drug tablet. The method used to separate the components was Thin Layer Chromatography (TLC) with silica adsorbent as the stationary phase and 0.5% glacial acetic as the mobile phase. In one plate‚ five known samples were used as the reference‚ that is: Aspirin; Caffeine; Ibuprofen; and Salicylamide. Aspirin and Salicylamide were the only samples that fluoresced. On a second plate‚ the tablet sample was developed. The results of the lab showed
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slowly to induce crystal growth 3. filter resulting mixture to isolate crystals Reading: Mohrig‚ Hammond & Schatz Ch. 15 pgs 183-197 Ch. 10 pgs 104-113 Ch. 14 pgs 174-182 • Scale: 5-10 mg discovery based research - a new material prepared in a lab 1‚000 kg + commercial applications - sugar refining‚ synthesis of pharmaceutical agents‚ etc. Recrystallization Recrystallization • Molecular selection - based on size‚ shape‚ & functionality • Molecular selection - based on size‚ shape
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COLUMN AND THIN LAYER CHROMATOGRAPHY Mark Paul P. Pastrana‚ Mariah Ericka M. Patawaran‚ Princess Juneire M. Peligro‚ Francisco Q. Pua III‚ Rose Anne L. Quyo and Janille P. Ragpa Group 8 2B Medical Technology Organic Chemistry Laboratory ABSTRACT The main objectives were to separate the colored components of malunggay leaves by means of column chromatography‚ as well as to determine the purity of the components using thin layer chromatography (TLC) and measure the Rf values of the colored
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IDENTIFICATION OF UNKNOWNS BY TLC AND MP IN COMBINATION Introduction: Thin layer chromatography (TLC) is one of the most valuable techniques in organic chemistry. This is a best method of separating and identifying mixtures of two or more compounds. The separation is accomplished by the distribution of the mixture between two phases: one that is stationary and one that is moving or mobile. Chromatography works on the principle that different compounds will have different solubilities
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