The main focus of this experiment was to find the relationship between temperature and the enzyme activity of amylase. This was achieved by attaining amylase enzyme‚ starch solution and potassium iodide (determines if enzymes hydrolyses the starch solution)‚ water bath and a hot plate. The temperatures used for this experiment were room temperature‚ 37oC‚ 60oC‚ 80oC‚ and 90oC. The hypothesis developed was that as the temperature increased‚ so will enzyme activity. Therefore‚ the ability of the
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Starch/Amylase Experiment Report Objective: The purpose of the starch/amylase experiment was to simulate and observe the process of enzyme digestion. Materials: * 1 small beaker * 2 large beakers * 2 cut pieces of soaked dialysis tubing * 2 dialysis tubing clamps or pieces of twine * 2 clean plastic pipettes * 1 bottle of Lugol’s solution * 2 glucose test strips Procedure: Begin the experiment by placing 4 full pipettes worth of cooked starch in a beaker. Then‚ use
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Error Analysis Lab By: Lab Team 5 Introduction and Background: In the process of learning about the importance of measurement and data processing‚ lab teams were given prompts to design experiments as well as address the precision‚ accuracy‚ and error analysis within the experiment. Lab teams collaborated their data to find similarities and differences within their measurements. Through this process‚ students learned the importance of the amount of uncertainty as well as the different
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Activity: Action Potentials Name: Caitlin Magnacca Instructor: Date: 03.19.2015 Predictions Exceeding threshold depolarization at the trigger zone______the likelihood of generation of an action potential. increases Action potential amplitude decreases with distance Increasing frequency of stimulation to the trigger zone increases number of action potentials Materials and Methods Experiment 1: Effect of Stimulus Strength on Action Potential Generation Dependent Variable membrane potential Independent
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of pH on amylase activity This practical allows you to: * discover how pH affects the rate of an enzyme controlled reaction * evaluate the experimental procedure Procedure SAFETY: Follow your teacher’s instructions for handling the solutions. Wear eye protection when handling the iodine solution. Investigation * Place single drops of iodine solution in rows on the tile. * Label a test tube with the pH to be tested. * Use the syringe to place 2 cm3 of amylase into the
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are found in and out of cells and lower the activation energy of a reaction. Amylase is the enzyme which catalyses starch hydrolysis. Alpha amylase and beta amylase are two types of amylase enzymes. The amylase which is the most commonly found in the human body is the alpha amylase. Beta amylase is mainly found in bacteria‚ fungi and plants. Amylase breakdown starch into maltose. During the process of hydrolysis‚ Amylase degrades starch by splitting the long glucose units into smaller intermediates
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PH level of Amylase Background info: What is Amylase? Amylase is an enzyme that helps digest carbohydrates. It is produced in the pancreas and the salivary glands. (Dugdale & Longstreth‚ 2011) Factors Affecting Amylase: Things that affect the efficiency of Amylase are temperature and pH levels. (Wikimedia Foundation‚ Inc‚ 2013) Function in the body: The function of Amylase in the human body is to break down plant-based starch sources. Therefore‚ providing the human body with more
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The Effect of Temperature on Animal and Fungal Amylase’s Ability to Breakdown Starch. Abstract This experiment was designed to test the reaction of the enzyme amylase at various temperatures. There were two different kinds of amylase being tested‚ one was fungal amylase also known as aspergillus oryzae and human amylase. The changes in temperature effect the rate at which an enzyme and a substrate collide. When the temperature is too high the active site changes shape or denatures‚ once this
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Activity of Salivary Amylase Domingo‚ Guray‚ Hugo‚ Lorenzo‚ Mohammad Isa Intro Because everything has a start Catalysis The process of increasing the rate of reaction with the use of a catalyst. Catalyst – any substance that increases rate of reaction upon addition to a certain reaction Page 3 Enzymes Act on substrates in a reaction Highly specific Breaks down complex macromolecules‚ synthesizes compounds essential for the cell Active site Enzyme-substrate
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1 The Limits of Amylase 2-1-14 Abstract This report explains the purpose of this experiment in a way that conveys information to the reader about Amylase’s ability to withstand acidic or basic pH. To do this‚ two test tubes were both filled with 5mL of a 5% amylase solution. The first one was filled with an acid‚ while the other was filled with a base. After dropping liquid Iodine and Benedict’s solution into each one‚ the tube with a basic pH tested positive for glucose. The acidic solution
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