prevalence of the Borrelia‚ Rickettsia and Ehrlichia species (the causes of the diseases mentioned above) in the tick A. inornatum‚ the Polymerase Chain Reaction (PCR) and Agarose Gel Electrophoresis procedures are applied. The PCR method is used to amplify the DNA samples of the tick studied‚ while the Agarose Gel Electrophoresis method is run in order to identify whether the species of bacteria of our interest are present in the DNA samples or not. The results obtained‚ so far‚ show 23.5% positive for
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The picture above shows a typical gel electrophoresis set up. The clear container in the center of the picture is called a gel electrophoresis chamber. It contains the agarose gel that will be loaded with genetic material‚ as well as a buffer solution. It is connected to a DC power supply via electrodes. This picture was taken at Paw Print Genetics laboratory in Spokane‚ Washington. Viney and Fenton (1998) defined the term electrophoresis as‚ “the migration of charged particles through a static medium
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Colon Cancer Scenario Introduction: Gel electrophoresis is an important molecular biology tool: it enables us to study DNA. It can be used to determine the sequence of nitrogen bases‚ the size of an insertion or deletion‚ or the presence of a point mutation; it can also be used to distinguish between variable sized alleles at a single locus and to assess the quality and quantity of DNA present in a sample. Gel electrophoresis is a method of separating chemical compounds and molecules by their size
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DNA Isolation and Visualisation The laboratory work today consists of three stages. 1. Separation and visualisation of DNA on agarose gel 2. DNA extraction 3. Virtual electrophoresis Part 1 involves casting a gel‚ which then requires approximately 30 minutes to set‚ followed by 1 hour to run. Consequently‚ you might want to cast the gel and let it set whilst you undertake Part 2 - your DNA extraction. Make
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Virology –Midterm vA‚ 22 April 2013: 1. Which of the following genomic nucleic acids are only found in viruses? a. dsDNA b. dsRNA c. ssDNA d. ssRNA e. B‚ C and D 2. About what percent of the human genome is indisputably viral? a. 1 b. 2 c. 5 d. 10 e. 50 3. Viruses were first discovered (and named as such) because they : a. could not be grown b. were very small c. were alive d. ate bacteria e. C and D 4. Phage therapy is to : a. Use a virus to kill a virus b. Use viruses to kill cancer cells c. Use
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4 Cardiovascular disorders CASE STUDY 19 Annie’s heartache Learning outcomes On completion of this case study‚ you will be able to: • describe the physiological characteristics of the coronary circulation; • outline the autonomic control of the heart and the mechanisms which control coronary blood flow; • review the causes of and possible treatments for angina; • describe the mechanisms of action of the following anti-anginal drugs: nitrates‚ beta-adrenoceptor antagonists and calcium
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Question 1 1.1 Silica gel chromatography: This is known as the stationary phase in column chromatography. Firstly‚ the tapered exit of the column is sealed using porous material. This porous material serves as support for the packing material‚ and prevents it from exiting the pipe. Thereafter‚ silica gel is compacted into the glass pipe to make the separating column. In finishing preparation of the column‚ the solvent which is used as the mobile phase is then passed through the dry column. Then the
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experiment‚ students were introduced to DNA electrophoresis. DNA electrophoresis is an instrument that many forensic scientists use to get a DNA fingerprint as an evidence for crimes. Not only can it be used for forensic science‚ people can use this for paternity test‚ as well as look for evolutionary relationships among organisms. Agarose is used to make the gel that the DNA fragments are going into. Since DNA particles are negatively charged‚ the gel is placed in a chamber with positively and negatively
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DIGE-based PTMs Analysis of protein post-translational modifications using DIGE-based proteomics Robert M. DeKroon‚ Jennifer B. Robinette‚ Cristina Osorio‚ Sun Yong Jeong‚ Eric Hamlett‚ Mihaela Mocanu and Oscar Alzate Summary Difference gel electrophoresis (DIGE) is most often used to assess relative changes in the expression levels of individual proteins in multiple complex samples‚ and this information is valuable in making inferences about relative protein activity. However‚ a protein‟s activity
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Materials and Methods Growing the G Strain and Preparing the GCE (rGFP Crude Extract): To grow the bacterial culture‚ use 10 ml of liquid LB growth media for incubation. 500 ml of the bacterial culture is allowed to grow overnight at 37°C. It is later shaken vigorously to increase the OD600 to 0.5‚ which means that time equals zero. At time zero‚ 1 mL of the culture is transferred into a 1.5 mL centrifuge tube and centrifuged for 5-10 minutes to obtain a pellet. The supernatant should be discarded
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