IODIMETRIC AND IODOMETRIC METHOD SUBSTANCE TO BE ASSAY AQUEOUS/NON-AQUEOUS ALKALINITY / ACIDIMETRY DIRECT/RESIDUAL TITRATION TITRANT INDICATOR CHEMICAL REACTION Assay of Antimony potassium tartrate Direct titration 0.1 N Iodine Starch TS KOOCCHOHCHOHCOO (SbO) + I2 + H2O KOOCCHOHCHOHCOO (SbO2) + 2HI + 2HI + 2NaHCO3 2NaI + 2H2O +
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The Effect of Osmosis on Potatoes/ Diffusion of Molecules through Benedicts Test of Dialysis dubing Introduction The focus of the lab on September 16‚ 2013 was Diffusion and Osmosis. Osmosis is a process in which the molecules of a solvent diffuse from an area of high concentration to an area of lower concentration‚ through a semipermeable membrane. Small solute molecules and water molecules can move freely through a selectively permeable membrane‚ but large molecules may pass through more
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The effect of size on the effectiveness of diffusion Aim: To use agar blocks infused with 0.1 Molar sodium hydroxide (NaOH) and phenolphthalein to investigate the relationship between shape and surface area: volume ratio on the effectiveness of diffusion. Hypothesis: That for a cube of agar‚ the time taken for complete colourisation due to diffusion of HCl is directly proportional to the cubes volume. Materials: |A block of agar (10cm x 5cm x 3cm) with 0.1M NaOH and |1x 250mL
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report Aim : To check the presence of starch in the leaves Hypothesis : the presence of starch can be detected if the specimen turns black blue after iodine solution. Materials : 1) Green leaves 2) Wire gauze 3) Iodine solution 4) Forceps 5) Specula 6) Bunsen’s burner 7) Test tube 8) Water 9) Tile 10) tripod stand 11) glass 12) slide 13) dropper 14) water 15) water bath Procedure: collect dark green leaves (as leaves contain starch) take a water bath and let it heat with
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glucose-1-phosphate‚ 1% maltose‚ honey‚ 1% sucrose‚ 1%lactose‚ 1% glycogen‚ 1% starch‚ protein‚ beer‚ distilled water and an unknown solution (test tube: 300). The iodine test for starch was to test how would starch reacted if we put iodine in it. The color of starch before the test was clear. The color of the iodine was brown. When you added iodine into starch‚ the result was the starch solution turned dark blue. Starch had a positive result is because of the complex of iodine stuck inside the amylase
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Introduction This practical experiment was performed in an attempt to observe how enzymes (starch phosphorylase in particular) are affected by varying its temperature before introducing it to the substrate it will be reacting with. A catalyst (enzyme) is a substance that changes the rate of a reaction; for a reaction to take place at all‚ the enzyme must first come into contact with the substrate. Enzymes are subject to a number of factors which effect how fast they can cause a reaction with a substrate;
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PLANNING COURSEWORK- STARCH AND AMYLASE AIM The aim of this coursework is to investigate the effect of temperature change‚ on the rate of hydrolysis of starch catalysed by amylase. PREDICTION I think that as the temperature increases‚ the rate of reaction also increases‚ to a point when it dramatically decreases. On graph 1‚ you will see a sketch of the graph which I expect to be the result of the experiment. SCIENCE REASONING I think my prediction is correct because the rate of
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What amount of amylase is the best to produce a better reaction with starch? A 1% solution of amylase‚ made from 0.15g of amylase added to 15ml of water‚ a 2% solution of amylase made of 0.3g of amylase added to 15ml of water‚ and a 0.5% solution of amylase made of 0.75g of amylase to 15ml of water were created. A 0.25% starch solution was also created‚ and 3ml was added to each of the 4 test tubes. 1 drop of iodine was added to each test tube. 4ml of the 1% solution was added to the first test
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with kidney disease each year‚ and some individuals face a difficult situation concerning the possibility of doing dialysis‚ and choosing what type of dialysis treatment is right for the individual. Both Hemodialysis and peritoneal dialysis give patients the quality of life. In contrast both procedures are done differently‚ Hemodialysis patients are dependent‚ and peritoneal dialysis patients are self-sufficient. In comparison both removes waste from the bloodstream; however‚ both procedures are beneficial
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enzyme for starch digestion‚ salivary amylase may well represent a potential compensatory alternate pathway for the digestion of amylose‚ amylopectin‚ and glycogen. Clinically significant depression of pancreatic amylase occurs in chronic pancreatitis‚ pancreatic resection‚ pancreatic neoplasm‚ cystic fibrosis‚ and other causes of pancreatic insufficiency. Quantitation of salivary amylase in these diseases has yet to be performed. It is also unclear whether the presence of starch in a meal
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