The Effect of temperature on beetroot This experiment is designed to investigate the effect of varying temperature (centigrade) on beetroot and its cells. My earlier experiment suggest that as temperature increases the integrity of the beetroot cell membrane will be destroyed and a subsequent release of beetroot pigment will be released into its surrounding milieu(in this case distilled water). In the experiment I will examine if my hypothesis (above) is correct. In order to conduct this experiment
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cuvettes. Put the distilled water into the colorimeter and calibrate it and then put the polluted water into the colorimeter to see the pH value. After that‚ create a filter and pour the polluted water into the filter to clean it. After this is complete‚ take the cleaned water and put it back into a clean cuvette. Then go back to the colorimeter and put the distilled water into the colorimeter and calibrate it and then put the filtered water into the colorimeter to find the
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40°C‚ 50°C‚ 60°C‚ 70°C) • Plastic Beaker (~250cm3) • 2 Boiling Tube Racks • 8 Boiling Tubes • Crushed Ice • 8 Thermometers (one per water bath) • Stopwatch • Distilled Water • Pipette • Cuvette • Colorimeter Method (N.B. – All personal protective equipment must be worn and care must be taken when using the cork borer or Scalpel/knife. All laboratory rules must be followed) Eight cylinders where cut from the Beetroot‚ using a cork
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in sulphuric acid to create the blue solution of copper. We put the blue solution of copper in the colorimeter and measured the absorbance of the solution 3 times then we found the average of the 3 different results we had gotten. We then diluted the blue solution of copper with water and put it into the colorimeter and measured the absorbance 3 times again and got the average again. A colorimeter is a light-sensitive instrument that measures how much colour is absorbed by an object or substance
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determined the concentration of a unknown CuSO4 solution by measuring its absorbance with the colorimeter. With all the calculations we were able to solve the linear regression Equation of absorbance vs. concentration and the alternate method. Materials Vernier LabPro or CBL 2 interface .40 M CuSO4 solution Computer or handheld CuSO4 unknown solution Vernier Colorimeter Pipet pump or pump bulb one cuvette
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Temperature Meter | 5800 | ACS | PH Electrode | 1000 | ACS | Temp. Probe | 650 | ACS | Electrode Stand | 450 | | COLORIMETER | | MT-107 | Microprocessor Photo Colorimeter‚ 8 Filter | 9200 | MT-108 | Deluxe Photo Colorimeter‚8 Filter | 7500 | MT-109 | Digital Hemoglobin Meter | 7000 | MT-110 | Auto Photo Colorimeter | 7200 | MT-111 | Digital Photo Colorimeter | 6200 | | CONDUCTIVITY METER | | MT-112 | Microprocessor Conductivity TDS/Temp. Meter | 12000 | MT-113 | Digital
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temperature of water will be increased in increments of 10oC‚ thus the temperatures I will be using are 20oC‚ 30oC‚ 40oC‚ 50oC and 60oC. Dependent Variable: I will be measuring the cell membrane permeability through the amount of pigment dispersion. A colorimeter will be used to quantify results into a percentage transmission of light‚ after it is left for 5 minutes. Control Variables: Variable How it is measured Why it needs to be measured Mass of beetroot cylinder A weighing scale should be used to ensure
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very similar structure to chymotrypsin; the Trypsin we used was from a beefs pancreas. Apparatus used in this investigation 1. Acidified protease solution 2. Milk powder 3. Standard glass wear 4. Stop Clock 5. Cuvette 6. Colorimeter 7. Measuring cylinder Before making my hypotheses I investigated what affects rates of reactions and the enzyme structure. The specific shape of the protein determines the active site for specific reactions‚ which brings me onto the lock
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chromaticity diagram and used to find % purity and dominant wavelength. The dominant wavelength is analogous to hue in the Munsell system and the distance of the object coordinates from white light is the %purity (Nielsen‚ 2010). The Agtron E-5 colorimeter measures the green/red ratio in samples. Generally‚ the redder a sample is the lower the Agtron’s reading (Mitchell‚ 2014). The Macbeth light box‚ which uses two spinning USDA Grade A and Grade C standards‚ can compare and evaluate samples with
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Solution: Beer’s Law Purpose: The purpose of the experiment is to determine the concentration and formula of an unknown cobalt nitrate solution by measuring absorbance. Introduction: A Colorimeter will be used to determine the concentration and formula of an unknown cobalt nitrate solution. The colorimeter sends blue light from the LED light source to pass through the solution and hit a photocell. A solution with a higher concentration will absorb more light and transmits less than s solution
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