This experiment is to prove Beer lamberts law‚ which states that absorbance is proportional to concentration. Part one materials 1. Colorimeter 2. Methyl orange 3. Potassium permangate 4. Cuvette 5. Deionised water Part one methods 1. Rinse one cuvette with deionised water 2. Fill one cuvette with deionised water and “zero” the colorimeter machine 3. Fill the cuvette that was rinsed with 4ppm Methyl orange 4. Record the absorbance values for 4ppm Methyl orange‚ making
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objective of this experiment is to determine the concentration of an unknown copper sulfate solution. You will be using the Colorimeter. In this device‚ red light from the LED light source will pass through the solution and strike a photocell. A higher concentration of the coloured solution absorbs more light (and transmits less) than a solution of lower concentration. The Colorimeter monitors the light received by the photocell as either an absorbance or a percent transmittance value. You are
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unknown copper (II) sulphate solution. You will be using the colorimeter. In this device‚ red light from the LED light source will pass through the solution and strike a photocell. The CuSO4 solution used in this experiment has a deep green color. A higher concentration of the colored solution absorbs more light (and transmits less) than a solution of lower concentration. [Content Standard B- Structure and properties of matter] The colorimeter monitors the light received by the photocell as either an
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cutting of beetroot‚ so wash of initial pigment (excess juice) at the start. Apparatus Pipette Scalpel Safety goggles Thermometer Water Bath 3 250ml beakers Colorimeter Tweezers Stopwatch Tile Ruler 19 test tubes (9 in water bath‚ 9 for when needed for colorimeter and so does not contain specimen‚ and 1 for resetting the colorimeter). 10ml measuring cylinder 10mm cork borer Test tube
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A light beam is passed through a sample inserted into a colorimeter‚ the light is split into two beams‚ one being absorbed by the sample‚ the other beam will pass through to a photocell which measure the amount of light absorbed. This figure is not measured in any unit but is an indication of the amount of light absorbed when referenced against other samples. Equipment Potassium Manganate (100g/ml)‚ distilled water‚ colorimeter‚ cuvettes‚ test tubes‚ test tube rack‚ burette‚ pipette. Part
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oxidase‚ the browner the substance will turn‚ and the faster it will achieve the color. In the present lab‚ different concentrations of catechol oxidase were mixed with pure catechol and the rate at which each solution browned was measured using a colorimeter. The results showed that the solution with the high concentration of catechol oxidase had the fastest rate at which it turned brown. However‚ it did not turn the brownest‚ the solution with the medium concentration of enzyme did. These results show
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and fragility of erythrocytes.With the exception of the method for determining erythrocyte fragility‚ conventional hematological laboratory procedures were used. The fragility technique consisted of introducing 0.4 cc. oxalated blood into a colorimeter tube containing 8 cc. of sodium chloride solution. The saline solution and blood were
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of the experiment is to find out the effect of salt concentration in a beetroot at a different temperature. The experiment will help to identify the effect of salt concentration in beetroot at different temperatures. After this‚ equipment called colorimeter will be used to take a reading for absorbency. The readings for the absorbency will be repeated three times in order to find the mean of each sample and to also find out the standard deviation of each set of data which will be recorded in a table
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Trail 5: k=33.787 Average Rate Constant: Trial1+2+3+4+5=145.774/5=29.1548 Procedure Obtain and wear goggles. Connect a Colorimeter to Channel 1 of the Vernier computer interface. Connect the interface to the computer with the proper cable. Start the Logger Pro program
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SPECTROPHOTOMETRY Spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength It involves with the use of a spectrophotometer. A spectrophotometer is a photometer that can measure intensity as a function of the light source wavelength. A spectrophotometer is commonly used for the measurement of transmittance or reflectance of solutions‚ transparent or opaque solids‚ such as polished glass‚ or gases. Principles
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