Blue white selection is a widely used method in screening recombinants in cloning. This is based on the gene product of lac z gene. The plasmid vectors contain this gene which produces β galactosidase enzyme. When a gene is inserted close to lac z gene‚ the reading frame will be distorted and the gene is inactivated. So the transformed cells will not produce this enzyme and are called competent cells. After the recombination‚ the bacterial cells are grown in a medium containing X gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside)
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Treatment Permanently Changing Brown Eyes to Blue Tired of having boring‚ brown eyes? Well a new innovation developed by Stroma Medical’s Dr. Gregg Homer can change just that. A new treatment that only takes an estimated time of 20 seconds can permanently change your brown eyes to blue. This is a new step forward in confirming to the beauty at any cost. This laser treatment will give you the piercing stare of mesmerizing eyes and give you the ever wanted blue eyes! Careful‚ though‚ as experts
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Annotated Bibliography: The power struggle in “Sonny’s Blues” Baldwin‚ James. Sonny ’s Blues. 10th ed. N.p.: publisher‚ 1957. Print. This article discusses how an unnamed narrator in “Sonny’s Blues” discovers from a newspaper that his younger brother‚ Sonny‚ has been arrested for selling and using heroin. It shows a inside view of the narrators feeling towards his brother Sonny. In the story “Sonny’s Blues” you will see how concerned he becomes about his brother. You will see a great deal of
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The Color Blue I. Positive associations 1. Blue is successful in bathrooms. 2. Is a peacemaker of color; not many people dislike blue. 3. Is considered a noble color‚ represents dignity and poise. 4. Dark blue inspires confidence and trust. 5. Pale blue is believed to encourage fantasy. 6. Cool‚ calm‚ comfortable‚ relaxing‚ and clean. II.
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LADY SINGS THE BLUES What is a song but a poem set to music? Take away the music from a good song and the rhythm of the words will create its own musical sound. "Songs For a Colored Singer"‚ a poem written by Elizabeth Bishop‚ is a song without the music. Bishop’s use of repetitive rhymes creates the lyrical‚ song like‚ structure to her poem. The voice of the song belongs to a black woman who encounters adversity throughout the poem. The sum of the elements‚ a black woman singing about hard times
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The Blue Jay (Cyanocitta cristata) The blue jay (Cyanocitta cristata) belongs to the family Corvidae and the order Passeriformes (Cornell University‚ 2015). It is one of the most common birds to be sighted in the United States. It has a range that covers about 2/3 of North America (Johnson‚ 2011). Blue Jays tend to migrate only over short distances‚ and have never been observed anywhere south of the United States. Given their wide distribution pattern‚ many observations relating to their life history
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drug addiction. Sonny and his brother’s relationship is nowhere close to perfect but tries to prove that people can change. In Sonny’s Blue’s‚ Baldwin uses symbols of Jazz and Blues music‚ and colors of light and darkness to show their brotherly relationship and their capability of having a good relationship. Jazz and Blues music symbolizes differently for Sonny and his brother. After the death of their
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Questions 1. A patient contracts hepatitis from contaminated food. During the acute (icteric) phase of the patient’s illness‚ the nurse would expect serologic testing to reveal A antibody to hepatitis D (anti-HDV). B hepatitis B surface antigen (HBsAg). C anti-hepatitis A virus immunoglobulin G (anti-HAV IgG). D anti-hepatitis A virus immunoglobulin M (anti-HAV IgM). Correct 2. The nurse determines that administration of hepatitis B vaccine to a patient has been effective when a specimen
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Brandon Smith ENG 2233 A Mrs. Lowe 2 December 2009 Sweet Blues With all the different forms of literature today‚ and allowing for the variety of genres available to today’s readers‚ there are endless possibilities of options regarding a reader’s favorite piece of literature. Many of my favorites can be categorized into the country-western genre; however‚ my taste in literature is not limited to stories of cowboys and Indians. As I have aged‚ I have found that I enjoy reading literature for
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Sita Sings the Blues: Four Connecting Movies in One Sita Sings the Blues is an animated version of the epic Indian tale “Ramayana”. The movie was released on February 11‚ 2008 and directed‚ produced‚ and written by Nina Paley. Sita Sings the Blues uses images and art covering two thousand years of history and culture. The movie is not just an average movie with one plot‚ but‚ instead‚ it is four stories each connecting and showing how ideals about gender roles have changed over time. Paley uses
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