The Observation of Bacteria in Pond Water, Cheek Cells, and E. Coli, S. Aureus, M.smegmatis under Compound Light Microscope by Using Staining Technique
The Comparative Analysis of Simple Staining and Gram Staining Techniques by observing E. Coli and S. Pyogenes under the Compound Light Microscope
INTRODUCTION:
A German bacteriologist, Dr. Theodore von Escherich, was the first man in 1885 who discovered the bacterium named Escherichia coli, which are gram negative and appears in rod shaped. Most kind of bacteria E. Coli does not cause diseases and some strains indeed are beneficial in helping the process of food breaking down in the intestines. However, “the most infamous strain E. Coli O157:H7”, which caused the outbreak of Jack in The Box hamburger in 1993 and the recent spinach outbreak in 2006, spread out abdominal pain and diarrhea on the civilization (American et al., 2011). Streptococcus pyogenes is a spherical, gram-positive bacterium, which was discovered by Hippocrates, who was known as “Father of Medicine” in the fifth century B.C. (Leyro et al, 2008). Streptococcus pyogenes affects its hosts in many different ways and causes large ranges of diseases, which includes both mild and serve disease, such as such as fever, severe pain, dizziness, and red rash. S. Pyogenes can destroy both red blood cells and white blood cells, which is responsible for being immunization (Todar et al, 2012). Both of bacteria, E.Coli and S. Pyogenes, were observed under the compound light microscope, which is a type of microscope using visible light and a system of lenses to magnify the images. In this second lab experiment, four slides of bacteria were viewed under the compound light microscope from 40X up to 400X total magnification for the purpose of giving the images of bacteria in detail beautifully. Moreover, the most important part of the experiment was the studying of being familiar to the two primary staining techniques, which were simple staining and gram staining. In the simple staining technique, the chemical methylene blue helped penetrating the cell wall and allowed the cell to be visible. Last
INTRODUCTION:
A German bacteriologist, Dr. Theodore von Escherich, was the first man in 1885 who discovered the bacterium named Escherichia coli, which are gram negative and appears in rod shaped. Most kind of bacteria E. Coli does not cause diseases and some strains indeed are beneficial in helping the process of food breaking down in the intestines. However, “the most infamous strain E. Coli O157:H7”, which caused the outbreak of Jack in The Box hamburger in 1993 and the recent spinach outbreak in 2006, spread out abdominal pain and diarrhea on the civilization (American et al., 2011). Streptococcus pyogenes is a spherical, gram-positive bacterium, which was discovered by Hippocrates, who was known as “Father of Medicine” in the fifth century B.C. (Leyro et al, 2008). Streptococcus pyogenes affects its hosts in many different ways and causes large ranges of diseases, which includes both mild and serve disease, such as such as fever, severe pain, dizziness, and red rash. S. Pyogenes can destroy both red blood cells and white blood cells, which is responsible for being immunization (Todar et al, 2012). Both of bacteria, E.Coli and S. Pyogenes, were observed under the compound light microscope, which is a type of microscope using visible light and a system of lenses to magnify the images. In this second lab experiment, four slides of bacteria were viewed under the compound light microscope from 40X up to 400X total magnification for the purpose of giving the images of bacteria in detail beautifully. Moreover, the most important part of the experiment was the studying of being familiar to the two primary staining techniques, which were simple staining and gram staining. In the simple staining technique, the chemical methylene blue helped penetrating the cell wall and allowed the cell to be visible. Last