Preview

Macconkey Agar Lab Report

Satisfactory Essays
Open Document
Open Document
235 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Macconkey Agar Lab Report
In this experiment, it started with cultured media and sterilisation of bacteria. The medium provided were MacConkey Agar, Nutrient Agar, Nutrient Broth. Type of sterilisation that being used was dry heat via flaming, by heating the coil till ember to destroy material that been effected with other organisms. The next experiment are focus on isolation and purification of microbes. After that, transfer cultured from broth into broth was done and follow up by slant agar Then, streaking technique used in Nutrient Agar while spreading bacteria used from broth to MacConkey Agar. Lastly, autoclave was done by lab assistant.
Escherichia coli was first isolated in 1885 from children's faeces by the German bacteriologist Theodor Escherich. It is

You May Also Find These Documents Helpful

  • Good Essays

    G Straine Lab Report

    • 1558 Words
    • 7 Pages

    To grow the bacterial culture, use 10 ml of liquid LB growth media for incubation. 500 ml of the bacterial culture is allowed to grow overnight at 37°C. It is later shaken vigorously to increase the OD600 to 0.5, which means that time equals zero. At time zero, 1 mL of the culture is transferred into a 1.5 mL centrifuge tube and centrifuged for 5-10 minutes to obtain a pellet. The supernatant should be discarded. The centrifuge with the bacterial pellet is labeled “G0” and stored at -20°C. The culture is induced with 1 Mm of IPTG and allowed to keep growing. After 3 hours past induction, 1 mL of the culture is pelleted into a different 1.5 mL centrifuge tube, and the bacterial pellet is labeled “G3.” The centrifuge with G0 bacterial strain needs 15 mL of the strain to be collected and to be pelleted into the centrifuge tube. The last pellet is labeled “G3-15 mL.” Finally, both the G3 and G3-15 mL are to be stored at -20°C.…

    • 1558 Words
    • 7 Pages
    Good Essays
  • Satisfactory Essays

    Finally, the agar plate is observed after 48 hours in the incubator. Data is recorded for each antibiotic by measuring, in millimeters, the cloudy space from the antibiotic disk, out. After data is recorded, the nutrient agar plate will be collected for safe disposal. Hands must be washed thoroughly with soap after handling bacteria…

    • 297 Words
    • 2 Pages
    Satisfactory Essays
  • Better Essays

    Purpose: The purpose of the this experiment is to learn to transfer microorganisms to a nutrient medium to promote growth using aseptic techniques. which are a method used to prevent unwanted organisms from contaminating a medium. We will also learn about different types of culture media, including MRS broth and nutrient broth, learn about oxygen and temperature requirements for microbial growth and how to control microbial growth.…

    • 1308 Words
    • 6 Pages
    Better Essays
  • Powerful Essays

    Macconkey Agar Lab Report

    • 2267 Words
    • 10 Pages

    The purpose of this study is to differentiate and identify two unknown organisms provided by the instructor in a nutrient broth. It is only known that the two organisms are from vomit; one is gram-positive and the other is gram-negative. It is necessary to first separate the two organisms by inoculating a nutrient agar plate using the streak-plate method. The initial streak-plate procedure was performed and placed in the incubator at 37◦C for 24-48 hours. Upon observing the growth on this plate, it is fairly obvious that one of the organisms is Serratia marcescens. However,…

    • 2267 Words
    • 10 Pages
    Powerful Essays
  • Good Essays

    ka lab report

    • 692 Words
    • 3 Pages

    We will be using the LoggerPro and LabPro in order to help us determine our data. The purpose of this experiment is to follow the changes of pH during the titration of an acid and a base in order to determine the of the weak acid, . is a constant for a given acid at a given temperature. In this experiment we determined the Ka using two different methods: 1) the measurement of the pH of a solution containing a known concentration of a weak acid, and 2) measurement of the pH at the half-neutralization point in the titration of a weak acid and a strong base.…

    • 692 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Gram Staining Lab

    • 423 Words
    • 2 Pages

    A Petri plate containing nutrient agar was used in the experiment. A sharpie was used to section off four quadrants of the dish for the different bacteria. Cotton swabs were used to sample different areas of the building. Cotton swabs used by me and my lab partner were used to test areas from the hallway water fountain, vending machine, hallway floor, and the door handle to enter the classroom. Once done with this process, the cotton swabs were swiped on the four different quadrants of the Petri plate, covered and sealed, and set aside for a week to let the bacteria grow on the agar.…

    • 423 Words
    • 2 Pages
    Good Essays
  • Good Essays

    12 Agar Plates Lab Report

    • 454 Words
    • 2 Pages

    For this experiment you will need 39 agar plates, sterile cotton swabs, a concentrated amount of Escherichia coli (E. coli), three different levels of antibiotic (Ampicillin: 1mg, 5mg, and 10mg), a bunsen burner, metal tweezers, an incubator, 27 broths, and a ruler which should all be provided through your laboratory. For the first generation, you will be using 12 plates. Three of the plates will be labeled G1 control, three of the plates will be labeled G1/1 mg, three plates will be labeled G1/5 mg, and the last three plates will be labeled G1/10 mg. Then you will dip a sterile cotton swab into the E. coli solution and you will streak one of the twelve agar plates. Repeat this step with a new cotton swab each time until all the plates have been streaked with…

    • 454 Words
    • 2 Pages
    Good Essays
  • Powerful Essays

    lab4

    • 1760 Words
    • 7 Pages

    Bacteria are inoculated (introduced) and cultured (grown) in the laboratory for test studies to determine their morphology (the shape, size, arrangement, and internal structures) and pathology (ability to cause disease). Inoculation has to be performed without adding other microbes or contaminants. Aseptic (sterile) technique is the process of growing pure (uncontaminated) cultures, and is essential for proper characterization of a bacterium. Aseptic technique includes the use of sterile media and equipment. The sterility of these tools is maintained by proper handling procedure, which prevents the introduction of contamination. The basics of aseptic technique are:…

    • 1760 Words
    • 7 Pages
    Powerful Essays
  • Satisfactory Essays

    Lab 4

    • 890 Words
    • 4 Pages

    Purpose: The purpose of this experiment is to Learn and employ aseptic technique, to become familiar with basic requirements of microbial growth, to learn the basic forms of culture media, and to become familiar with methods used to control microbial growth.…

    • 890 Words
    • 4 Pages
    Satisfactory Essays
  • Good Essays

    E.coli or binomial name Escherichia coli was discovered by a German pediatrician named Theodore Escherich in 1885. Dr. Escherich originally named the bacteria, bacillus communis coli. After the demise of Dr. Escherich the intestinal bacteria was then named Escherichia coli after the late doctor in 1919.…

    • 695 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Unknown Microorganism

    • 1335 Words
    • 6 Pages

    The streak for isolation was chosen to see the formation of the different colonies and to see if the bacteria would cause growth on the plate. After observing the plate, it could be seen that the unknown had growth and formation of colonies. The Gram stain was chosen next to determine if the unknown was gram-negative or gram-positive. After looking at the gram stain under the microscope with the oil immersion it could be determined that the unknown was gram-negative due to the fact that the bacteria was pink in color and rod shaped. After determining the unknown was gram-negative, other bacteria’s were able to be eliminated from the options. The Triple Sugar Iron Test was chosen next because the unknown could be tested for glucose fermentation, lactose fermentation, sucrose fermentation and sulfur reduction. If the organism is able to ferment glucose and lactose and/or sucrose it will turn the medium yellow throughout (Leboffe & Burton 329). When the Triple Sugar Iron Test was complete, it showed a yellow butt, yellow slant and gas production. This determined that the unknown ferments glucose and one or both of the other sugars. The gas was produced by fermentation. The color change in the agar brought the unknown down to two options of unknowns, Escherichia coli or Hafnia alvei. The SIM medium test was chosen next to see sulfur reduction, indole production from tryptophan…

    • 1335 Words
    • 6 Pages
    Good Essays
  • Good Essays

    The bacteria was removed from a single loopful of broth from the tube and streak it into…

    • 698 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    Unknown Bacteria

    • 544 Words
    • 3 Pages

    In this laboratory exercise explore the differences of microorganism and continue our use of specialized media and use some biochemical testing.This report discloses the basic laboratory instruments will be used in each of our practices .It is of great importance to recognize and identify the different instruments and laboratory tools, because in this way will we be able to use them properly and also to call them by name and know why.…

    • 544 Words
    • 3 Pages
    Satisfactory Essays
  • Good Essays

    Timeline of Genetics

    • 2240 Words
    • 9 Pages

    1855 The Escherichia coli (E. Coli) bacterium is discovered. It later becomes a major research, development and production tool for biotechnology.…

    • 2240 Words
    • 9 Pages
    Good Essays
  • Powerful Essays

    Obtain two nutrient agar plates and using a permanent marker draw four quadrants on the bottom of each agar plate. Using a sterile pipet transfer 250 ml of E. coli broth to the middle of each petri dish and evenly spread bacterial culture around the agar plate. Cover and allow the culture to soak into agar for at east 15 minutes. Using sterile forceps, carefully place one filter disk from designated sample into the middle of each…

    • 939 Words
    • 4 Pages
    Powerful Essays