Preview

Light Reaction Lab

Good Essays
Open Document
Open Document
477 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Light Reaction Lab
Light Reactions Lab
This lab explores the effect of light intensity and light wavelength on photosynthesis. The quantity of energy [ATP] produced will change depending upon on these parameters.
Use "Johnson Explorations: Photosynthesis" located at http://www.mhhe.com/biosci/genbio/biolink/j_explorations/ch09expl.htm Examine the diagram before clicking "skip intro" so you have an idea what is going on. The animation show the movement of photons, electrons, water, protons, ATP etc. relating to the ETC of a thylakoid.
Procedure: Use the simulation to determine how wavelength and light intensity affect the rate of photosynthesis [measured by ATP production]. You are dealing with two variables you must keep one variable constant while manipulating
…show more content…
Introduction: Describe briefly the light dependent reactions and propose a hypothesis to answer the experimental question.
2. Procedure: Briefly describe the simulation, and the method for obtaining your data.
3. Data: Include data tables for the simulation for both changes of intensity and changes of wavelength. More than one table is probably required.
4. Graphs showing how the percentage of ATP changed as a result of changes in wavelength and intensity . Use a spreadsheet or graphing program of your choice.
3. Conclusion: Use your data to answer the experimental question. Answer clearly how light wavelength affects the reaction, and how light intensity affects the reactions. Offer an explanation of the results, taking into account the principles of photosynthesis and the light reaction.
Grading Rubric Poor (1 pts) Satisfactory (2 pts) Good ( 3 pts)
Introduction Explanation of light dependent reaction lacking and/or hypothesis missing. Offers explanation of the reaction, some parts vague or incorrect. Hypothesis unclear or does not answer experimental question fully. Offers good explanation of the the light dependent reaction, includes how light is used to produce ATP and NADPH. Proposes hypothesis to answer experimental

You May Also Find These Documents Helpful

  • Good Essays

    In this lab, an Elodea specimen is submerged in water under a lamp with a filter that can change the color of the light. We will measure Elodea's rate of photosynthesis by measuring the amount of oxygen it produces. We will investigate the effects of light color and light intensity on Elodea's rate of oxygen production.…

    • 725 Words
    • 4 Pages
    Good Essays
  • Satisfactory Essays

    Photosynthesis Lab Report

    • 566 Words
    • 3 Pages

    Photosynthesis is a process to which some autotrophs such as plants produce their own food. It has two stages or reactions light-dependent and light-dependent reactions. The light dependent reactions are the first stage, where energy from sunlight is captures in Photosystem 2 and then 1, the electrons generated in Photosystem 1 then moves along the electron transport chain. The moving of electrons causes a hydrogen ion gradient that is used in the final step to produce ATP, by the ATP Synthase. The equation for this reaction is 6CO2 + 6H2O  C6H12O6+6O2 or, carbon dioxide +water sugars + oxygen . This reaction takes place on the thylakoid membrane of the chloroplast and uses energy from sunlight to produce compounds like ATP and NADPH. The second stage of photosynthesis is the Calvin Cycle, which takes place in the stoma of the chloroplast. This stage is light-independent or it does not need energy from sunlight. During this reaction ATP and NADPH from Stage 1are used to produce high energy sugars. The reaction is 3CO2 + 6NADPH + 5H2O + 9ATP  G3P + 2H+ + 6NADP+ + 9ADP + 8Pi . Additionally, the three limiting factors of photosynthesis are the light intensity, carbon dioxide concentration, and the temperature. Lastly, the items used in this particular experiment were baking soda to provide CO2, a light to excite the electrons in stage 1, distilled…

    • 566 Words
    • 3 Pages
    Satisfactory Essays
  • Good Essays

    Based off the results from this lab the student was able to determine and have an overall better understanding of the variables that affect the rate of photosynthesis in a plant. The first variable the student tested in the lab was 30cm of light with CO2 present. The student started this trial with all 10-leaf disks and timed how many leaf disks came up every minute for 15 minutes. One minute into the trial and the student recorded that no leaf disks had yet to rise to the top of the solution. At three minutes into the trial the student recorded that no leaf disks had still yet to rise to the top of the solution.…

    • 2047 Words
    • 9 Pages
    Good Essays
  • Good Essays

    Using molar extinction coefficient 0.014, calculate rate of reduction DCPIP per chloroplast. Plot (electron transfer) per minute vs light intensity.…

    • 956 Words
    • 4 Pages
    Good Essays
  • Better Essays

    Photosynthesis Lab Report

    • 874 Words
    • 4 Pages

    These experiments were designed to visualize the process of photosynthesis. We looked at how light was manipulated to affects the rate at which these reactions occur. With our results, we have a better understanding of…

    • 874 Words
    • 4 Pages
    Better Essays
  • Good Essays

    Photosynthesis lab

    • 625 Words
    • 3 Pages

    The objective of this study was to figure out how different light colors affected the rate of photosynthesis in spinach leaves. This was done by taking leaf disks, removing the CO2 and sinking them in beakers. One beaker filled with regular water, the other filled with a mixture of water and sodium bicarbonate. The beakers were then placed in front of the light sources and the amount of disks floating was recorded every minute. In our study the disks exposed to the red light started to float sooner than the…

    • 625 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    If i increase the intensity of the rate of photosynthesis would drastically increase which is the light bubbles would be emitted in the jar in one minute because of the fact that the more photons of light that fall on a leaf then more chlorophyll molecules would be ionised and the more ATP and NADPH are generated If i increase the intensity of the rate of photosynthesis would drastically increase which is the light bubbles would be emitted in the jar in one minute because of the fact that the more photons of light that fall on a leaf then more chlorophyll molecules would be ionised and the more ATP and NADPH are generated fadsfIf i increase the intensity of the rate of photosynthesis would drastically increase which is the light bubbles would be emitted in the jar in one minute because of the fact that the more photons of light that fall on a leaf then more chlorophyll molecules would be ionised and the more ATP and NADPH are generated…

    • 571 Words
    • 3 Pages
    Satisfactory Essays
  • Good Essays

    Photosynthesis Lab Report

    • 456 Words
    • 2 Pages

    When the wavelength increases and the light strength is at a constant, then the ATP will decrease, because the wavelengths will be longer and the energy would be less. While shorter the wavelengths are, the more ATP it has, or the more energy it contains. When the wavelength is at a constant measure and light intensity is increasing- ATP production will increase as…

    • 456 Words
    • 2 Pages
    Good Essays
  • Satisfactory Essays

    A prediction for this experiment would be that as different wavelengths are tested the rate of photosynthesis will either increase or decrease. Photosynthesis will also speed up when the light is…

    • 508 Words
    • 2 Pages
    Satisfactory Essays
  • Satisfactory Essays

    This is an investigation of the effect of the intensity of light (photosynthesis) have on the rate of cellular respiration (measured as the number of oxygen bubbles). We will determine whether or not it is possible to examine the relationship between photosynthesis and cellular respiration under controlled environmental conditions. We will show the effects, reactions, and relationship between photosynthesis and respiration, as well as demonstrate to you how the scientific method works.…

    • 358 Words
    • 3 Pages
    Satisfactory Essays
  • Good Essays

    cell energy

    • 475 Words
    • 3 Pages

    What is the role of the light reactions? Include the reactants and the products. Where does it occur?…

    • 475 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Absorbtion of Spinach

    • 847 Words
    • 4 Pages

    In the photosynthesis system, plants and other photosynthesizing creatures they use light of the sun as energy by absorbing some specific wavelength. A prepared sample of the chloroplast from spinach was used during this experiment. In order to observe the absorbance of chloroplast, a sample of the 7 mL chloroplast was prepared by pipetting the 7 mL amount of the chloroplast in to the clean, dry cuvette. A spectrophotometer was warmed up from the beginning of the lab to get prepared for the actual testing. A blank sample which contained 7mL sorbitol buffer was used to celibate the device between each different wavelength. the result shown on the spectrophotometer was recorded.…

    • 847 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Photosynthesis Lab Report

    • 1392 Words
    • 6 Pages

    Cited: "Lab #4 - Photosynthesis." Laboratory Exercises for Biology 101. Ed. Barbara Stegenga. N.p.: n.p., n.d. 24-28. Print.…

    • 1392 Words
    • 6 Pages
    Good Essays
  • Satisfactory Essays

    Rate of photosynthesis

    • 385 Words
    • 2 Pages

    Factors that could affect the rate of photosynthesis could be the duration of wavelength, high light intensity, or even air pollutants. The topic we researched was ‘High Light Intensity”. High light intensity happens beyond saturation point. Light intensity reduces the rate of photosynthesis. This affect is called solarization. It can…

    • 385 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Light is a factor required in order that photosynthesis can occur. In any series of chemical reactions where one substance combines with another to form a longer compound, energy is needed to fuel the reactions. Energy for photosynthesis is provided by light from the sun or from artificial lamps. As with carbon dioxide, the amount of light energy present is important in determining the rate of photosynthesis – simply, the more light or greater luminance absorbed by the plant, the more photosynthesis can take place.…

    • 693 Words
    • 3 Pages
    Good Essays