Lab Report On Blood Typing
The purpose of this experiment was to identify four synthetic blood samples through a process called blood typing. According to Fredrick H. Martin, PhD., et.al, blood typing is a test that determines a person’s blood type by checking the blood for the presence or absence of specific glycoprotein called antigens found on the surface of the red blood cells. The two clinically relevant antigens are the A antigen and B antigen. The blood type is named based upon which antigen is present on the surface of its red blood cells. There are four blood types. Blood type A has A antigens; blood type B has B antigens; blood type AB has both A and B antigens and blood type O has neither A nor B antigens on the surface of the red blood cells. An additional
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Instead of using one drop of synthetic blood sample and one drop of synthetic antiserum in each well, we used two drops of synthetic blood sample and two drops of synthetic antiserum in each well. We also did not rinse the well plate after conducting every test but we finished testing all the samples then rinsed all the blood typing slides at the same time.
Results
In sample 1, there was agglutination in well A and Rh, and there was no agglutination in well B. In sample 5 there was agglutination in well A, B, and Rh. In sample 3 there was agglutination in well B and Rh, and there was no agglutination in well A. In sample 4 there was agglutination in well B, and there was no agglutination in well A and Rh.
Sample 1 Sample 5 Sample 3 Sample 4
Data Table Representing the Results of the Synthetic Blood Typing
Well Antiserum Sample 1 Sample5 Sample 3 Sample4
A Anti-A Yes Yes No No
B Anti-B No Yes Yes Yes
Rh Anti-Rh Yes Yes Yes No Blood Type A+ AB+ B+ B-
“Yes” is for there was agglutination and “No” is for there was no agglutination. …show more content…
The specific clinical conditions that require blood typing are, during blood transfusion to prevent the risk of receiving an incompatible blood type, classification of the blood during blood donation, and Blood typing for pregnant women because if the mother is Rh negative and the fetus is Rh positive, a drug called Rhogam that keeps the mother’s body from producing antibodies that may attack the baby’s blood cells is administered to the mother (Martine, et.al, 2015, p.
Instead of using one drop of synthetic blood sample and one drop of synthetic antiserum in each well, we used two drops of synthetic blood sample and two drops of synthetic antiserum in each well. We also did not rinse the well plate after conducting every test but we finished testing all the samples then rinsed all the blood typing slides at the same time.
Results
In sample 1, there was agglutination in well A and Rh, and there was no agglutination in well B. In sample 5 there was agglutination in well A, B, and Rh. In sample 3 there was agglutination in well B and Rh, and there was no agglutination in well A. In sample 4 there was agglutination in well B, and there was no agglutination in well A and Rh.
Sample 1 Sample 5 Sample 3 Sample 4
Data Table Representing the Results of the Synthetic Blood Typing
Well Antiserum Sample 1 Sample5 Sample 3 Sample4
A Anti-A Yes Yes No No
B Anti-B No Yes Yes Yes
Rh Anti-Rh Yes Yes Yes No Blood Type A+ AB+ B+ B-
“Yes” is for there was agglutination and “No” is for there was no agglutination. …show more content…
The specific clinical conditions that require blood typing are, during blood transfusion to prevent the risk of receiving an incompatible blood type, classification of the blood during blood donation, and Blood typing for pregnant women because if the mother is Rh negative and the fetus is Rh positive, a drug called Rhogam that keeps the mother’s body from producing antibodies that may attack the baby’s blood cells is administered to the mother (Martine, et.al, 2015, p.