Introduction To The Microscope
Student Name
Motunrade obasun
Course
Human Biology
Date
04-11-2014
Introduction to the Microscope: Lab Report
From Exercise 1:
1. Draw what you see on the ‘e’ slide at 160 xs in the space below.
Snaps will be submitted separately.
2. Describe the relationship between what you see through the eyepiece and what you see on the stage.
On the stage it was not visible enough rather it looks too small, while in microscope it appears black and grainy.
3. Draw the image you see of the ‘e’ slide at 200x.
Snaps will be submitted separately.
From Exercise 2:
Describe what you see as you move through the layers of threads:
At a time only one thread can be focused and other two remains out of focus.
From Exercise 3:
Draw what you viewed when you observed your wet mount slide under the microscope.
.
Snaps will be submitted separately.
Post Lab Questions:
1. How do you calculate total magnification on a compound light microscope? How is the calculation of magnification different on the pocket microscope?
The total magnification in a light microscope is the product of the ocular and objective lenses.
Suppose
Ocular is of 10x
Objective lens is of 40 x.
Total magnification will be 10X40=400.
For pocket Microscope
M= (LENGHT OF MICROSCOPE TUBE)/(focal length OF EYE PIECE)*(focal length of objective lens)
2. What parts of a compound light microscope can be used to control the amount and intensity of light reaching the object being examined? How is the pocket microscope different? Adjustment of light in Compound Light microscope: In light microscope condenser controls the quality of light which reaches the slide and the diaphragm controls the quantity of light reaching the slide or stage. Diaphragm is needed to adjust only to control the amount of light reaching the slide. While the light microscope uses external light Bulb to illuminate the object being examined.
3. How does the orientation of an object being observed compare between a compound
Motunrade obasun
Course
Human Biology
Date
04-11-2014
Introduction to the Microscope: Lab Report
From Exercise 1:
1. Draw what you see on the ‘e’ slide at 160 xs in the space below.
Snaps will be submitted separately.
2. Describe the relationship between what you see through the eyepiece and what you see on the stage.
On the stage it was not visible enough rather it looks too small, while in microscope it appears black and grainy.
3. Draw the image you see of the ‘e’ slide at 200x.
Snaps will be submitted separately.
From Exercise 2:
Describe what you see as you move through the layers of threads:
At a time only one thread can be focused and other two remains out of focus.
From Exercise 3:
Draw what you viewed when you observed your wet mount slide under the microscope.
.
Snaps will be submitted separately.
Post Lab Questions:
1. How do you calculate total magnification on a compound light microscope? How is the calculation of magnification different on the pocket microscope?
The total magnification in a light microscope is the product of the ocular and objective lenses.
Suppose
Ocular is of 10x
Objective lens is of 40 x.
Total magnification will be 10X40=400.
For pocket Microscope
M= (LENGHT OF MICROSCOPE TUBE)/(focal length OF EYE PIECE)*(focal length of objective lens)
2. What parts of a compound light microscope can be used to control the amount and intensity of light reaching the object being examined? How is the pocket microscope different? Adjustment of light in Compound Light microscope: In light microscope condenser controls the quality of light which reaches the slide and the diaphragm controls the quantity of light reaching the slide or stage. Diaphragm is needed to adjust only to control the amount of light reaching the slide. While the light microscope uses external light Bulb to illuminate the object being examined.
3. How does the orientation of an object being observed compare between a compound