Experimentation
Exercise 1: Cell Transport Mechanisms and Permeability: Activity 2: Simulated Facilitated Diffusion Lab Report Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1. Molecules need a carrier protein to help them move across a membrane because You correctly answered: d. they are lipid insoluble or they are too large. 2. Which of the following is true of facilitated diffusion? You correctly answered: c. Movement is passive and down a concentration gradient. 3. Examples of solutes that might require facilitated diffusion include You correctly answered: d. all of the above. 4. Which of the following would not affect the rate of facilitated diffusion? You correctly answered: a. the amount of intracellular ATP
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Experiment Results Predict Question: Predict Question 1: What effect do you think increasing the number of protein carriers will have on the glucose transport rate? Your answer : a. The glucose transport rate will decrease. Predict Question 2: What effect do you think adding Na+ Cl- will have on the glucose transport rate? Your answer : c. There will be no change in the glucose transport rate. Stop & Think Questions: What happened to the glucose transport rate when the glucose concentration was increased from 2 mM to 8 mM? Your answer : c. The glucose transport rate was unchanged. Correct answer: a. The glucose transport rate increased. Describe the conditions that resulted in the fastest rate of glucose transport. You correctly answered: d. 700 glucose carrier proteins and 8 mM glucose Why do you think equilibrium wasn't reached? You correctly answered: b. The membrane proteins were saturated. Experiment Data: Run Number 1 2 3 4 5 6 6 Solute Glucose Glucose Glucose Glucose Glucose Na+ ClGlucose Start Conc. L 2.00 8.00 2.00 8.00 10.00 0.00 2.00 Start Conc. R 0.00 0.00 0.00 0.00 0.00 2.00 0.00 Carriers 500 500 700 700 100 700 700 Rate 0.0008 0.0023 0.0010 0.0031 0.0017# 0.0042 0.0010
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Experiment Results Predict Question: Predict Question 1: What effect do you think increasing the number of protein carriers will have on the glucose transport rate? Your answer : a. The glucose transport rate will decrease. Predict Question 2: What effect do you think adding Na+ Cl- will have on the glucose transport rate? Your answer : c. There will be no change in the glucose transport rate. Stop & Think Questions: What happened to the glucose transport rate when the glucose concentration was increased from 2 mM to 8 mM? Your answer : c. The glucose transport rate was unchanged. Correct answer: a. The glucose transport rate increased. Describe the conditions that resulted in the fastest rate of glucose transport. You correctly answered: d. 700 glucose carrier proteins and 8 mM glucose Why do you think equilibrium wasn't reached? You correctly answered: b. The membrane proteins were saturated. Experiment Data: Run Number 1 2 3 4 5 6 6 Solute Glucose Glucose Glucose Glucose Glucose Na+ ClGlucose Start Conc. L 2.00 8.00 2.00 8.00 10.00 0.00 2.00 Start Conc. R 0.00 0.00 0.00 0.00 0.00 2.00 0.00 Carriers 500 500 700 700 100 700 700 Rate 0.0008 0.0023 0.0010 0.0031 0.0017# 0.0042 0.0010
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