Experiment Chromotography
Experiment 9
Thin Layer Chromatography
Experiment: 9
Title: Thin Layer Chromatography
Introduction:
Chemical analysis is conducted on specific analytes (specific substance of interest in a mixture), however it is often found that these analytes must be separated for the chemical analysis to conduct their analysis.
Chromatography is a set up of laboratory a technique that is used to separate a chemical mixture. This technique is very useful as it allows us to follow the course of the reaction and separation of the product from the mixture. There are preparative and analytical Chromatography conducted, function of a preparative Chromatography is to separate the mixture for further usage allowing purification. Analytical Chromatography is done to evaluate the concentration of analyte is the small amount of solute.
The main component of the Chromatography consist of the mobile phase and also stationary phase. We can describe the mobile phase as the phase that moves through the stationary phase. The mobile phase can be a liquid, gas or a supercritical fluid.The mobile phase carries along the analyte that is needed to be separated or analyzed through a stationary phase which has different degree where the mixtures are absorbed at various rate.
Objective:
Part 1:
The aim for part 1 of the Thin Layer Chromatography is to learn the technique and visualization of the colorless compound. Secondly, is to identify the unknown compound based on the comparisons made with standard compound.
Part 2:
The aim of separation technique by Thin Layer Chromatography is to separate the mixture of individual compounds from the spinach leaf sample used.
Material and apparatus:
Part 1 : Analysis of Analgesic Drugs
Material:
Aspirin Acetaminophen Caffeine Unknown A Unknown B TLC plates Ethyl acetate Hexane Acetic acid Iodine
Apparatus: UV Lamp Capillary tube 250mL beaker Filter paper
Thin Layer Chromatography
Experiment: 9
Title: Thin Layer Chromatography
Introduction:
Chemical analysis is conducted on specific analytes (specific substance of interest in a mixture), however it is often found that these analytes must be separated for the chemical analysis to conduct their analysis.
Chromatography is a set up of laboratory a technique that is used to separate a chemical mixture. This technique is very useful as it allows us to follow the course of the reaction and separation of the product from the mixture. There are preparative and analytical Chromatography conducted, function of a preparative Chromatography is to separate the mixture for further usage allowing purification. Analytical Chromatography is done to evaluate the concentration of analyte is the small amount of solute.
The main component of the Chromatography consist of the mobile phase and also stationary phase. We can describe the mobile phase as the phase that moves through the stationary phase. The mobile phase can be a liquid, gas or a supercritical fluid.The mobile phase carries along the analyte that is needed to be separated or analyzed through a stationary phase which has different degree where the mixtures are absorbed at various rate.
Objective:
Part 1:
The aim for part 1 of the Thin Layer Chromatography is to learn the technique and visualization of the colorless compound. Secondly, is to identify the unknown compound based on the comparisons made with standard compound.
Part 2:
The aim of separation technique by Thin Layer Chromatography is to separate the mixture of individual compounds from the spinach leaf sample used.
Material and apparatus:
Part 1 : Analysis of Analgesic Drugs
Material:
Aspirin Acetaminophen Caffeine Unknown A Unknown B TLC plates Ethyl acetate Hexane Acetic acid Iodine
Apparatus: UV Lamp Capillary tube 250mL beaker Filter paper