Preview

Determine Disinfectant and Antiseptic Effectiveness Using Zone Inhibition Method

Better Essays
Open Document
Open Document
1275 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Determine Disinfectant and Antiseptic Effectiveness Using Zone Inhibition Method
Lab Report Title Page:

Determine Disinfectant and Antiseptic Effectiveness Using Zone Inhibition Method Prepared for: By: Date:

Introduction Page:

Abstract
This study is to examine the effects of different types of disinfectants by disk diffusion method using common agents such as Betadine, Clorox, Crest, Kiss My Face, Listerine, and Lysol. Wound care and general cleaning of home surfaces is a part of daily activity for many of the general population. Knowing which agents are effective against the bacteria that most commonly cause infection, disease, and illness allows educated decisions on which agents to use. We compared the bactericidal effectiveness of 6 common agents widely used by the general public against 3 common bacterial organisms.

Introduction Sterilization kills all organisms in or on an object or substance. Disinfectants and antiseptics do not sterilize since many types of organisms and spores are not killed. Disinfectants are described as antimicrobial agents that are used on inanimate objects such as an instrument or structural surfaces. The term antiseptic is usually applied to antimicrobial agents that are used on living tissue such as skin and throat mucosa.

A disinfectant must be capable of killing pathogens while it is in contact with them, so that they cannot grow again when it is removed. This action is said to be cidal (lethal), and it is described according to the type of organisms it kills as bactericidal, sporicidal, fungicidal, virucidal, etc… If the antimicrobial substance merely inhibits the organisms while it is in contact with them, they may be able to multiply again when it is removed. In this case, the agent is said to have static activity and may be described as bacteriostatic, fungistatic, etc…

Microorganisms of different groups are not uniformly susceptible to chemical disinfection due to the structures of their cell walls. As an example, Mycobacterium are more resistant



Cited: Pearson, 2010. Symbiosis Lab 16 Disk Diffusion Assay to Determine Disinfectant and Antiseptic Effectiveness, pp. 89-91. Pearson Custom Publishing Vicki S. Rabenberg, Christopher D. Ingersoll, Michelle A. Sandrey, and Mary T. Johnson. The Bactericidal And Cytotoxic Effects Of Antimicrobial Wound Cleansers. 2002. Centers for Disease Control and Prevention http://www.cdc.gov/nczved/divisions/dfbmd/diseases/ecoli_o157h7/index.html#spread Violet I. Haraszthy, DDS, MS, PhD; Joseph J. Zambon, DDS, PhD;Prem K. Sreenivasan, PhD. The Antimicrobial Efficacy of Commercial Dentifrices Featured in General Dentistry. 2010 Agarry O., Olaleye M.T, and Bello-Michael, C.O. Comparative antimicrobial activities of aloe vera gel and leaf. African Journal of Biotechnology Vol. 4 (12), pp. 1413-1414, 2005. ZDENKA CVETNI, ANDA VLADIMIR-KNE. Antimicrobial activity of grapefruit seed and pulp ethanolic extract. Department of Microbiology Faculty of Pharmacy and BiochemistryUniversity of Zagreb in conjunction with the Croatia and Department of Pharmacognosy Faculty of Pharmacy and Biochemistry University of Zagreb, Croatia. 2004.

You May Also Find These Documents Helpful

  • Good Essays

    Lab 3 Module

    • 821 Words
    • 4 Pages

    Sterilization is the complete destruction or elimination of all viable organisms in or on an object while disinfection is the destruction of pathogenic nonsporulating microbes or their toxins usually on inanimate surfaces.…

    • 821 Words
    • 4 Pages
    Good Essays
  • Powerful Essays

    Ebt1 Task 3

    • 1325 Words
    • 6 Pages

    A primary concern is post-operative infection. This is one of the most common complications of surgery and can have serious implications. Since post-operative infections account for one third of all nosocomial infections nationwide, this is an important issue to address. I believe that focusing on better ways to prevent possible post-operative infection would greatly improve surgical patients’ outcomes and experiences. As the procedure states now, the patient is required to take a shower/bath with normal bath soap excluding any lotions or powders prior to surgery and then once in the surgical site, is cleansed with Hibiclens and an intravenous antibiotic is initiated. This has been standard practice because past research suggested that as long as the patient bathed with normal soap and the site was scrubbed in surgery, post-operative infection has a decreased incidence. While this has proven to drastically reduce infection rate, there are more recent studies that have proven that bathing with normal soap alone is not sufficient enough to prevent a notable amount of nosocomial infections. Hibiclens is a prescription antimicrobial antiseptic skin cleanser used to cleanse a surgical site. It is an effective product; however, I have found that there are still post-operative infections occurring. For this reason, I decided to analyze the pre-operative procedure of surgical site…

    • 1325 Words
    • 6 Pages
    Powerful Essays
  • Powerful Essays

    If the ring of inhibition is greatest with the Aloe vera, then the plant A. vera is the best, as a smaller zone of inhibition means that there will be a lesser growth of bacteria when that herb is used in the actual experimentation. This comes from a previous study by Julia F. Morton (1961), which says that ‘In South Africa natives split the leaves of the Aloe saponaria Haw. and lay the fleshy surface on…

    • 5411 Words
    • 22 Pages
    Powerful Essays
  • Good Essays

    Microbiology

    • 1151 Words
    • 5 Pages

    Needed for this experiment, is a container of Proactive™ and a container of Clearasil™. Some tools needed are: a Bunsen burner (aseptic technique to keep hands sterile when sanitized), three sterile agar plates, a sharpie, sterile cotton swabs, two glass plates, tweezers, and sterile disks. Other materials needed are: Escherichia coli, Streptococcus epidermis, distilled water, ninety five percent ethanol,…

    • 1151 Words
    • 5 Pages
    Good Essays
  • Powerful Essays

    MICRO BIOLOGY

    • 1277 Words
    • 6 Pages

    Disinfectants are agents that destroy many of the components of the micro-organism at once. These disinfectant agents are composed of many different ingredients such as sterile water and surfactants. The disinfectants will be used against both gram negative and gram positive bacteria. And data will be collected as to how much the disinfectant can reduce the count of the bacterial organism with a contact time of five minutes. We will judge whether this specific disinfectant can prevent bacteria from spreading.…

    • 1277 Words
    • 6 Pages
    Powerful Essays
  • Powerful Essays

    QCF level 3 DIPLOMA unit 21

    • 2538 Words
    • 10 Pages

    UNIT 21: 1.1.1, 1.1.2, 2.2.1, 2.2.2, 3.3.1, 3.3.2, 4.4.1, 4.4.2, 4.4.3, 4.4.4, 5.5.2, 5.5.3, 5.5.4, 5.5.5.5, 5.5.6, 5.5.7, 5.5.8, 6.6.1, 6.6.3, 6.6.4, 6.6.5, 6.6.6…

    • 2538 Words
    • 10 Pages
    Powerful Essays
  • Good Essays

    Steriliant Cidex Problems

    • 663 Words
    • 3 Pages

    In a healthcare setting, it is important to be able to control infectious organisms. Sterilants and disinfectants are a crucial tool for meeting that. It is true that sterilants and disinfectants are toxic to living organisms but they can also be harmful to us healthcare providers. I myself work in a Surgery Center, where I come in contact with a sterilant called Cidex. This product is a powerful sterilant that is used to sterilize our instruments and to kill any living organism that may be living in the instrument and to have the instrument safely sterilized for another patient to be used on. Now it has been shown that using this product, Cidex has proven to show that healthcare professionals have health problems after being exposed to it or even long term exposure of Cidex. It is very important to follow all guidelines and precautions when using this type of sterilant in a work place.…

    • 663 Words
    • 3 Pages
    Good Essays
  • Better Essays

    Antiseptics are applied to the skin to reduce the possibility of infection. Common antiseptics include rubbing alcohol, boric acid, hydrogen peroxide and…

    • 1302 Words
    • 6 Pages
    Better Essays
  • Better Essays

    The fact that each bacterial species, and even some of their respective strains, responds uniquely to a given antimicrobial makes it necessary to have methods, which provide researchers and clinicians with measurable susceptibilities. This need has become prevalent since the rising of later generations of antimicrobial compounds achieved by chemical modifications of the naturally occurring antibiotics. By utilizing the Kirby-Bauer method, researchers and clinicians are able to determine the susceptibility of given microorganisms to different antibiotics and antimicrobials. While antibiotics specifically target bacteria, antimicrobials cast a wide net, nonspecifically targeting microorganisms and viruses. During this lab, we tested the effects of both antimicrobials and antibiotics on different bacterial cultures. By creating different lawns of bacteria and placing on each of them disks soaked in different antibiotics and antimicrobials, we were able to observe the susceptibility of the differing bacteria to several agents. This process that we practiced is the Kirby-Bauer method. As stated before, antibiotics and antimicrobials serve differing purposes. Antibiotics are substances, usually in the form of medication, that destroy bacteria or inhibit the growth of bacteria. On the other hand, antimicrobials act in destroying or inhibiting the growth of all microorganisms. One type of antimicrobial is an antiseptic, which acts to reduce the presence of microorganisms without harming living animal tissue (El-Mayas, 2013). It is important to note that while we were able to measure the susceptibility of these agents, we cannot conclude that resistance is present because further investigations would have to be conducted to determine if the microorganism has developed a metabolic capability to withstand the agent’s mechanisms and effects (Maxwell, 2013).…

    • 977 Words
    • 4 Pages
    Better Essays
  • Good Essays

    The incidents and increased complications of diseases in hospitals continue to escalate. Cross-contamination is a concern because the spread of microorganism from room to room can rapidly become a big outbreak. Understanding the modes of prevention is vital for the success against bacteria; these responsibilities apply to everybody working and visiting a hospital. Using a good hand washing techniques, proper wear of personal protective equipment and sterilization of surgical equipments are the first line of defense in preventing cross-contamination in a hospital setting.…

    • 717 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    U.S. Department of Health and Services, (2014). Disinfection By-Products. Atlanta: Centers for Disease Control and Prevention.…

    • 1591 Words
    • 7 Pages
    Powerful Essays
  • Better Essays

    Cross Contamination

    • 3817 Words
    • 16 Pages

    The level and quality of the use of the highest efficacy of high-level disinfectants in automated reprocessors was rated at acceptable credibility and fit. The intervention has shown that PHMB-DBAC provides the same efficacy as OPA. PHMB-DBAC is a superior product in reduction of contact time and side effects. Proposal to changing from OPA would entail a cost analysis of the…

    • 3817 Words
    • 16 Pages
    Better Essays
  • Best Essays

    Registered Nurse

    • 3806 Words
    • 16 Pages

    Popovich K Lyles R Hayes R Hota B Trick W Weinstein R Hayden M 2012 Relationship between Chlorhexidine Glucnate Skin Concentration and Microbial Density on the Skin of Critically Ill Patients Bathed daily with Chlorhexidine Gluconate.Popovich, K., Lyles, R., Hayes, R., Hota, B., Trick, W., Weinstein, R., & Hayden, M. (2012). Relationship between Chlorhexidine Glucnate Skin Concentration and Microbial Density on the Skin of Critically Ill Patients Bathed daily with Chlorhexidine Gluconate. Infection Control and Hospital Epidemiology, 33(9), 799-896. 20130414120625108042359…

    • 3806 Words
    • 16 Pages
    Best Essays
  • Powerful Essays

    WHO (2002) antimicrobial (will slip through our grasp says WHO) The Pharmaceutical Journal 264 (7101) pp 902…

    • 6153 Words
    • 25 Pages
    Powerful Essays
  • Good Essays

    Antiseptics

    • 701 Words
    • 3 Pages

    An antiseptic is a substance which inhibits the growth and development of microorganisms. For practical purposes, antiseptics are routinely thought of as topical agents, for application to skin, mucous membranes, and inanimate objects, although a formal definition includes agents which are used internally, such as the urinary tract antiseptics.…

    • 701 Words
    • 3 Pages
    Good Essays

Related Topics