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Daphnia Lab Report

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Daphnia Lab Report
Does caffeine affect the heart rate of Daphnia?

Introduction In this experiment I aim to find out if caffeine has an affect on heart rate. Instead of using human subject, whom may already have a high tolerance to caffeine or could have ingested caffeine that day making the tests unfair, I will use Daphnia, a crustacean. They have on contact with caffeine making it fair. It will be easier to observe as it has a translucent body, making it able to see the heart beating under a microscope. It will be a "blind study" to avoid bias results, so everyone will be unaware what the solution consists of.

Purpose The purpose of my experiment was to determine how caffeine affected the heart rate of Daphnia.

Hypothesis I believe that a Daphnia subjected to
…show more content…
The cells in the table that have been highlighted seem to be anomalies thus to keep the experiment fair I did not take these two results into account when producing my average heart rate over the two minutes and the average bpm. Including these two results in the averages and graph would unbalance the whole experiment and probably have a significant affect on the way the graph should really look. Using these results my Hypothesis would argue that solution 5 should have the highest concentration with an average of 218 bpm. The lowest bpm was 148 this would be expected to have the lowest concentration of caffeine in it.
After the results had been collected the concentration of caffeine in each solution were given to us, the table will now need to be validated to show the concentrations of each solution.

Heart rate 2 mins 2 mins 2 mins 2 mins 2 mins Average over the 2 mins Average

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