Bacteria and Plasmid to Produce Red Fluorescent Proteins
Bacterial Transformation Bacteria and plasmid to produce Red Fluorescent Proteins Alejandra Lopez Biology 124L
Abstract
A transformation in the literal sense of the world was witnessed in 1928 by Fredrick Griffith. A living organism had changed in physical form. The purpose of this study is to produce recombinant DNA molecules to produce bacteria that would transform into red fluorescent proteins. One plasmid was that allowed to express a red fluorescence was produced by recombining two plasmids by using molecular techniques. Agar plates labeled LB, LB/AMP, and LB/AMP/ARA containing ampicillin (AMP) and arabinose (ARA) were used to grow of the bacteria of interest and SDS-PAGE gels were utilized in identifying the fluorescent and non-fluorescent proteins. The end results illustrated that there were no signs of fluorescent proteins in the gel bands and there were red fluorescing bacteria in the LB/AMP plate that should not have been. The agars contained in all three plates was exposed to arabinose, and there is a possibility that the plates were labeled incorrectly causing the unsuccessful results.
Introduction According to the hypothesis proposed by George Beadle and Edward Tatum in 1940, the transforming principle involved one or more genes to produce enzymes needed to synthesize the polysaccharide coat. Biochemical tests revealed it to be deoxyribonucleic acid (DNA). Watson Crick Discovered the elegant structure of the DNA and the molecular genetics was born which eventually lead to
Abstract
A transformation in the literal sense of the world was witnessed in 1928 by Fredrick Griffith. A living organism had changed in physical form. The purpose of this study is to produce recombinant DNA molecules to produce bacteria that would transform into red fluorescent proteins. One plasmid was that allowed to express a red fluorescence was produced by recombining two plasmids by using molecular techniques. Agar plates labeled LB, LB/AMP, and LB/AMP/ARA containing ampicillin (AMP) and arabinose (ARA) were used to grow of the bacteria of interest and SDS-PAGE gels were utilized in identifying the fluorescent and non-fluorescent proteins. The end results illustrated that there were no signs of fluorescent proteins in the gel bands and there were red fluorescing bacteria in the LB/AMP plate that should not have been. The agars contained in all three plates was exposed to arabinose, and there is a possibility that the plates were labeled incorrectly causing the unsuccessful results.
Introduction According to the hypothesis proposed by George Beadle and Edward Tatum in 1940, the transforming principle involved one or more genes to produce enzymes needed to synthesize the polysaccharide coat. Biochemical tests revealed it to be deoxyribonucleic acid (DNA). Watson Crick Discovered the elegant structure of the DNA and the molecular genetics was born which eventually lead to
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