Thin layer chromatography Essays & Research Papers

Best Thin layer chromatography Essays

  • Thin Layer Chromatography and Column Chromatography
    Introduction Chromatography is a technique used to separate a mixture into its individual components. In this experiment, chromatography is done by using a thin layer chromatography (TLC) plate and filter paper. The word ‘chromatography’ itself means the separation of colours. However, nowadays, both coloured and colourless compound are used in chromatography by various methods. In chromatography, the separation process of mixture is mainly based upon the polarity and molecular weight of...
    2,559 Words | 9 Pages
  • Thin Layer Chromatography of Analgesics
    Thin-Layer Chromatography of Analgesics Abstract: In this experiment thin-layer chromatography (TLC) was used to determine the composition of an unknown mixture of analgesics given five references of AC, ASP, CAF, IBU, SAL, an unknown consisting of a mixture of three of the references, and two unknowns (6,23) containing a single reference compound. The composition of the unknown mixture and two single unknowns were determined by spotting the reference compounds and the unknown compounds on...
    627 Words | 2 Pages
  • Thin Layer Chromatography - 535 Words
    It was concluded that the product created was paracetamol when a thin layer chromatography test was performed and observed under ultraviolet light (which is discussed further down the page). An unknown substance was treated with acetic anhydrate and resulted with paracetamol. A compound that behaves in this matter is 4-aminophenol and is widespread in the industrial production of this drug. Upon these observations was the build up of “Chemical A” decided. When 4-aminophenol is t treated...
    535 Words | 2 Pages
  • Thin Layer Chromatography - 1618 Words
    INTRODUCTION The purpose of this experiment is to identify the components of an unknown analgesic drug substance by comparing the Rf values of its composition compounds with those of reference compounds using thin layer chromatography. The unknown to be identified is unknown #101. This thin layer chromatography experiment is made of two major parts: The first part, samples of the unknown solution and the standard solutions are spotted on a TLC plate that we dip into a developing chamber...
    1,618 Words | 8 Pages
  • All Thin layer chromatography Essays

  • Thin Layer Chromatography - 1909 Words
    Experiment 5 Title : Thin Layer Chromatography Objectives: i. To distinguish polar and non-polar solvents. ii. To familiar with the analysis technique by using the thin layer chromatography. iii. To differentiate the retention factor, Rf for different compounds. [pic] Result: |Compound |Distance traveled by the compound | |o-nitroanaline...
    1,909 Words | 7 Pages
  • Column and Thin Layer Chromatography
    =========== ORGANIC CHEMISTRY ============ Organic Chemistry Laboratory - CH 201 (2010-2011) Experiment 4B Column and Thin-Layer Chromatography of Capsicum Frutescens L. Pigments John Cyril Abanto*, Vernalyn Abarintos and Clarice Gail Abella Department of Chemistry, College of Science University of Santo Tomas, Espana Street, Manila 1050 Date Submitted: September, 2010...
    1,089 Words | 5 Pages
  • Column and Thin Layer Chromatography
    COLUMN AND THIN LAYER CHROMATOGRAPHY Group 9 RAMOS, Sharina Joy; REYES Aina Marie; REYES Jallisa Maan; RUBIO, John Michael; SABINO Patricia Anne; SANTOS, Carlos Rafael ABSTRACT To separate the colored components of siling labuyo and to determine the purity of the components, the students performed a column and thin or solid-liquid chromatography procedure. The solid may be almost any material that does not dissolve in liquid phase. But for this experiment, the solid used by the students...
    1,489 Words | 5 Pages
  • Thin Layer Chromatography - 1227 Words
    Making TLC Plates from Bulk TLC Silica Gels Many TLC users prefer to use pre-coated TLC plates, but others because of their special needs, or because they need special additives or a special thickness, coat their own TLC plates. This paper is a quick review of what is needed for the process. Initial instructions for plate coating: If reproducible plates are needed, then the user should invest in a coating device from Camag or Desaga (addresses below). The devices consist of a tray that...
    1,227 Words | 4 Pages
  • Thin Layer Chromatography Lab
    Thin Layer Chromatography Lab November 30, 2011 Calculations Substance | Distance moved by Substance | Distance moved by Solvent | Rƒ | Methyl Orange | 3.4 cm | 4.5 cm | .75 | Methyl Violet | 3.6 cm | 4.5 cm | .80 | Indigo Carmine | 3.4 cm | 4.5 cm | .75 | Sudan IV | 4.6 cm | 5 cm | .92 | Methylene Blue | 3.7 cm | 5 cm | .74 | “Unknown” I | 4.1 cm | 4.5 cm | .91 | “Unknown” II | 4.1 cm | 4.5 cm | .91 | Methyl Orange moved with the solvent not leaving a trail. Instead the...
    576 Words | 2 Pages
  • Thin Layer Chromatography - 1550 Words
    ------------------------------------------------- Thin layer chromatography From Wikipedia, the free encyclopedia Thin-layer chromatography | Separation of black ink on a TLC plate | Acronym | TLC | Classification | Chromatography | Other techniques | Related | Agarose gel electrophoresis SDS-PAGE | TLC of three standards (ortho-, meta- and para-isomers) and a sample Thin layer chromatography (TLC) is a chromatography technique used to separate non-volatile mixtures.[1] Thin...
    1,550 Words | 5 Pages
  • Thin Layer Chromatography - 564 Words
    Discussion Using the Thin Layer Chromatography experiment, caffeine was found to be the most polar due to the fact that it stayed closer to the stationary phase. Caffeine contains four amine groups that are extremely polar as a result of the hydrogen bond and amide functional group. Acetaminophen was found to be the second most polar analgesic drug tested. Acetaminophen contains a polar alcohol group on one side and amide group on the other but also includes non-polar functional groups that...
    564 Words | 2 Pages
  • Thin Layer Chromatography - 758 Words
    Date:7.6.2013 Objectives: Part 1: (i)To learn the technique of TLC and the visualization of colourless components. (ii)Identify the unknown drug by a TCL comparison with standard components. Part2 :to learn the separation technique by using TLC plate in separating a mixture of compound by using spinach leaf. Apparatus:UV lamp,capillary ,250 ML beaker Materials:aspirin,acetaminophen,caffeine,unknown A,unknown B,TLC plates,ethyl acetate,Hexane,acetic acid,iodine Introduction:...
    758 Words | 3 Pages
  • Thin Layer Chromatography - 1909 Words
    Thin Layer Chromatography Submitted in partial fulfillment of the requirements for: CHE 324 Organic Chemistry Laboratory Dr. Robert Duncan Fall Semester 2012 Caitlin Inman, Team Leader October 9, 2012 Tyler Byrd, Data Collection Shared Role, Technique Expert Introduction: “Chromatography is used to separate components of a mixture. For example, imagine a mixture of wood pieces, pebbles, and large rocks to be separated and the chromatography setup as a stream. Flowing...
    1,909 Words | 6 Pages
  • column and thin layer chromatography
    Column and Thin Layer Chromatography Beverly Abstract: Plant pigments were separated and concentrated from a crude spinach extract through the use of column chromatography and an eluatropic series of hexanes, hexane/acetone, and methanol. The pigments were analyzed using thin layer chromatography with a 30% ethyl acetate/hexane developing solvent. Introduction: Chromatography is a technique used to separate a mixture of two or more components based...
    2,274 Words | 8 Pages
  • Thin Layer Chromatography of the Unknown Analgesic
    Thin Layer Chromatography of the Unknown Analgesic Jessica Bajao*, Phoebe Abalos, Kevin Antiga, Carmelus Aseneta 3-Biology 2 College of Science, University of Santo Tomas, Manila, Philippines Abstract The group used five different analgesics in this experiment: aspirin, acetaminophen, ibuprofen, caffeine, and mefenamic acid. Six analgesics were spotted on the TLC plate including the unknown. After the development of the TLC plate, it was placed under ultraviolet light for the spots...
    1,192 Words | 5 Pages
  • Thin Layer Chromatography Lab Report
    OBJECTIVE The purpose of this experiment is to analyze mixtures of compounds prior to, during and after a separation scheme. This experiment also allows monitoring reactions of organic molecules, and determines the identity of a mixture of compounds. STRUCTURES AND PHYSICAL PROPERTIES OF REACTANTS [1] SOLVENTS | a. Hexane1Molecular Molarity: 86.18 g/molBoiling Point: 69 ºCMelting Point: -95ºCDensity: 0.659 g/mL at 25ºCWater Solubility: Insoluble in waterColor/Texture:...
    1,791 Words | 5 Pages
  • Experiment 9 Thin-Layer Chromatography
    Title: Thin-Layer Chromatography Objectives: Part1: 1. To learn the technique of TLC and the visualization of colourless components. 2. To identify an unknown drug by a TLC comparison with standard compounds. Part 2: To learn the separation technique by using Thin Layer Chromatography plate in separating a mixture of compounds into individual pure compound by using Spinach Leaf. Introduction: (i) General Concepts Chromatography is a common and powerful method used to separate and analyze...
    3,875 Words | 13 Pages
  • Thin Layer Chromatography Lab Report
    Thin Layer Chromatography Introduction Thin Layer Chromatography or TLC is a technique used as a separation and identification technique. There are many forms of chromatography, but one thing that remains constant throughout all of the types of chromatography is that there is a stationary phase and a mobile phase. In the case of TLC the stationary phase is the silica gel on the TLC tray. Procedure Chromatograph method is a method of separating mixtures of two or more compounds. Two...
    522 Words | 2 Pages
  • Advantages and disadvantages of paper chromatography and thin layer chromatography
    Advantages of thin layer chromatography (TLC) TLC is very simple to use and inexpensive. Undergraduates can be taught this technique and apply its similar principles to other chromatographic techniques. There are little materials needed for TLC (chamber, watch glass, capillary, plate, solvent, pencil, and UV-light). Therefore, once the best solvent is found, it can be applied to other techniques such as High performance liquid chromatography. More than 1 compound can be separated on a TLC...
    385 Words | 2 Pages
  • Column and Thin Layer Chromatography: the Separation of Spinach Pigments
    Title Page Chelsea Maksin Organic Chemistry Laboratory 1 Column and Thin Layer Chromatography: The Separation of Spinach Pigments Russ Hoburg 02/15/2012 Abstract The main goal in doing the column and thin layer chromatography was to separate spinach extract into its components based on polarity and then to analyze the components. The separation of the spinach extract was done using the column chromatography with the wet/slurry packing method. Alumina was inserted into the column to act...
    3,467 Words | 10 Pages
  • Column and Thin Layer Chromatography: the Separation of Spinach Pigment
    Column and Thin Layer Chromatography: The Separation of Spinach Pigment Abstract Spinach extract was separated into fractions containing compounds of similar polarity by column chromatography. Based on solid-liquid phase partitioning, this separation technique exploited the different polarity of the compounds in the spinach extract. Three fractions with different colors were obtained. The extract and its fractions were analyzed using thin layer chromatography (TLC). The TLC results showed...
    1,250 Words | 4 Pages
  • Expt 81 Thin Layer Chromatography TLC
     Expt. 8.1 Thin Layer Chromatography (TLC) Analysis of Analgesics Krista Jinks CH 211L Section F 10/27/14 I. Abstract: The objective of this experiment was to analyze analgesic tablets by TLC to determine what analgesics they contain. The analgesics that could be in the tablets included ibuprofen, acetaminophen, aspirin, and caffeine. Through the use of TLC, unknown substance #62 was found to be Bayer Aspirin because it only contained aspirin. II. Introduction: 1....
    650 Words | 4 Pages
  • The Preparation of Acetaminophen (Paracetamol) with Thin Layer Chromatography (Tlc) to Monitor the Reaction.
    Experiment 3: The preparation of acetaminophen (paracetamol) with thin layer chromatography (TLC) to monitor the reaction. Abstract: This experiment is to demonstrate the preparation of paracetamol and its properties. Reflux and filtration of 4-aminophenol and acetic anhydride formed the crude sample. Further analysis of dry white crystals were used to give quantitative measurements and a percentage yield of 46% was obtained. The overall conclusion is that the acetic anhydride reacted...
    2,108 Words | 10 Pages
  • Thin-Layer Chromatographic Analysis
    Experiment #1: Thin-Layer Chromatographic Analysis of Drug Components | Performed on: Wednesday, May 8th, 2013 | | | 5/15/2013 | | Introduction: The goal of this experiment was to identify an unknown analgesic drug through the use of thin-layer chromatography. The drugs used were, Unknown #1, caffeine, aspirin, and acetaminophen. Thin-Layer Chromatography is a technique for identifying substances using a mobile phase (developing solvent) that draws up through...
    951 Words | 4 Pages
  • The Separation of Spinach Pigments via Column and Thin-Layer Chromatography
     The Separation of Spinach Pigments via Column and Thin-Layer Chromatography 5/29/14 Abstract: Spinach extract was separated into fractions containing compounds of similar polarity via column chromatography. Thin layer chromatography was then used to analyze the extract and its separated components. Experimental results showed that the retention factor for carotene (0. 0.86) was the largest, followed by xanthophyll (0.38) and then chlorophyll (0.14)...
    2,524 Words | 8 Pages
  • Thin Layer and Column Chromatography: Extracted Total Lipids of Chicken Egg Yolk
    Thin Layer and Column Chromatography of Extracted Total Lipids of Chicken Egg Yolk Aegan Matthew V. Amican, Karen Gem B. Ares, Ruvie Ann A. Ballester, Mark Joseph S. Barcelona,* Katherine Carmen Isabel G. Calleja, Christelle Venus F. Capuno, Group 1, 2DPH, University of Santo Tomas Abstract Lipids are one of the major constituents of foods, and are important in our diet for a number of reasons. They are a major source of energy and provide essential lipid nutrients. This experiment...
    1,773 Words | 5 Pages
  • Drugs Screen Using Thin Layer Chromatography of Basic Illicit Drugs
    Drugs screen using Thin Layer Chromatography of basic illicit drugs Abstract The majority of evidence submitted to crime labs comes from drug-related crimes. Often, this evidence includes unidentified powders that may be illegal drugs. In order to prosecute individuals for possession of illegal substances, it is necessary for forensic scientists to positively identify any suspected drugs submitted to the laboratory. In addition, forensic toxicologists must determine the...
    1,226 Words | 5 Pages
  • chromatography - 1280 Words
    Column and Thin layer Chromatography of Red Siling Labuyo Marian Angelu Ramos, Rose Ann Refuela, Leomarie Duanne Sanchez, Paula Lynne Santos, Geraline Sarmiento, Jon Carlo Semana Group number Seven, 2E- Pharmacy, Faculty of Pharmacy, University of Santo Tomas ABSTRACT Chromatography is the separation of mixtures into their constituents. It relies on the differential solubilities or absorptivities of the components to be separated with respect to two phases, one of which is stationary and...
    1,280 Words | 6 Pages
  • Chromatography - 993 Words
     Chromatography – Analyzing Analgesics by TLC and Isolation of β-Carotene by Column Chromatography Introduction/Background: Flavonoids are an important group of additives that can be defined as pure substances either natural, extracted from raw materials or synthetic. Chromatography is the separation of two or more compounds or ions caused by their molecular interactions with two phases – one moving and one stationary (Weldegirma 2012). Three types of...
    993 Words | 7 Pages
  • Chromatography - 360 Words
    Paper chromatography is an important separation technique that depends upon differences in how strongly the dyes are adsorbed onto the paper (stationary phase) and how soluble the dyes are in the developing solvent (mobile phase). In paper chromatography, a small amount of the mixture to be separated is placed close to the edge of a piece of paper. The edge of the paper is then immersed in a developing solution. As the developing solution ascends up the paper by capillary action, the. components...
    360 Words | 2 Pages
  • Chromatography - 2000 Words
    COLUMN AND THIN LAYER CHROMATOGRAPHY Maria Janine B. Abarientos, Kuia B. Allarde, Aliana Keshia P. Andino Mary Viadelle E. Andrada and Nina Marian Robelea G. Ang Group 1 2C Pharmacy Organic Chemistry Laboratory ABSTRACT In this experiment, the techniques column and thin layer chromatography was used to separate and determine the purity of the colored components of siling labuyo and malunggay leaves. The results obtained relied on differential solubilities and adsorptivities of the...
    2,000 Words | 8 Pages
  • Chromatography - 1688 Words
    Chromatography Abstract Paper chromatography is one of the methods under chromatography, it can use in identifying unknown compounds using known compound and it can also use as a separation technique based on the differences in affinities of components of the mixture to a stationary phase and a mobile phase. In the experiment, the stationary phase was the filter paper onto which the dye samples were dropped onto while the mobile phase was the solvent mixture containing ethanol and water...
    1,688 Words | 6 Pages
  • Chromatography - 884 Words
    Chromatography: How can we separate a mixture? Purpose The chromatography lab is to understand how molecules with similar molecular properties can be separated with paper chromatography. These differences will be interpreted to see the distinction of separate chemical substances. Pre Lab Questions 1. Explain capillary action as it pertains to water and paper. Capillary action makes water draw up the paper. As paper absorbs water mixes with the solutions in the paper. 2. What is...
    884 Words | 5 Pages
  • CHROMATOGRAPHY - 502 Words
    OBJECTIVES: The objective of this experiment was to extract plant leaf pigments and determining them by using the Rf values obtained from the paper chromatography technique. The hypothesis of the experiment was that all of the five listed pigments would be present in the extracted plant leaf according to the Rf values. PROCEDURE/APPARATUS: The equipments used were a 18 x 150 mm test tube with stopper, graduated cylinders, Erlenmeyer flask, mortar and pestle, metric ruler, tall jar, acetone,...
    502 Words | 2 Pages
  • Chromatography - 969 Words
    Paper chromatography is one of the easiest methods of chromatography. It is a method of planar chromatography (stationary phase is in form of a plane). Paper chromatography follows the basic principle of chromatography, which states that substances or components are distributed in between the stationary phase and the mobile phase. It is an analytical technique, where only a small amount of a sample is used for separating and identifying its components. Like any other method of chromatography,...
    969 Words | 3 Pages
  • Thin-layer Chromatogragaphy : Analysis of Drug Components
    --- Experiment 1 : Thin-layer Chromatogragaphy : Analysis of Drug Components CHEM221 Date experiment performed : January 20, 2014Date Submitted : January 27, 2014 Introduction This laboratory has its first objective to familiarize with the thin layer chromatography (TLC) technique. Second, each student has to identify the unknown components by comparing it to one of the tree dissolved liquid analgesics: Acetaminophen, Aspirin and Caffeine. Finally, calculating the Rf value by...
    1,084 Words | 4 Pages
  • Lab #1 Extraction and Thin Layer Chromotography
    Lab #1 Extraction and Thin Layer Chromatography Kaya Gaudet 6784928 January 9, 2013 Line Structure References BenzophenoneBiphenylBenzoic Acid Extraction: A process used to separate different compounds in a mixture based on their solubility in an immiscible substance. Usually an aqueous and organic phase are used. During extraction the desired compound moves one phase and leaves the unwanted substance behind. Extraction normally has to be done a few times to leave all of the...
    1,617 Words | 5 Pages
  • Paper Chromatography - 525 Words
    A solution of the mixture to be separated is "spotted", usually from a micropipet, near one edge of a piece of filter paper, and the solvent is evaporated. Usually several sample and standard spots are placed along the edge. Then the chromatogram is "developed" by immersing that edge of the paper in a solvent that migrates through the paper as the mobile phase. The solvent often has two, three or four components, one of which is usually water. Development is normally done in a chamber that is...
    525 Words | 2 Pages
  • Experiment on Chromatography - 2527 Words
    ------------------------------------------------- Using Liquid-Liquid Partitioning Chromatography Teshia Faye T. Josue Institute of Chemistry, University of the Philippines, Diliman, Quezon City July 10, 2013 July 16, 2013 I. II. Methodology Chromatography is a method of separation that has been familiar to students from the time they take up Chemistry in their high school years. Specifically though, it was the Paper Chromatography that was introduced. Different colors of inked...
    2,527 Words | 8 Pages
  • Candy Chromatography - 305 Words
    Have you ever wondered how many dyes are in your favorite candies? Candy chromatography is a method of separating and comparing dyes used in candy. It is a complex mixture, without having to taste it. The word chromatography is broken into two Greek words, “ chroma” meaning color and ‘graphein’ meaning to write. It works because the components of the mixture will be different in how much they “stick” to each other, and to other substances. The dyes that stick more tightly to the paper fibers...
    305 Words | 1 Page
  • paper chromatography - 595 Words
    Paper chromatography Presented by – Miss. Shruti Vilas Kharat. SYBSc / FS13164 History- Paper Chromatography (PC) was first introduced by German scientist Christian Friedrich Schonbein (1865). PC is considered to be the simplest and most widely used of the chromatographic techniques because of its applicability to isolation, identification and quantitative determination of organic and inorganic compounds. Definition- Paper chromatography is an analytical method technique for separating...
    595 Words | 3 Pages
  • Paper Chromatography - 588 Words
    Analysis of Food Colorings by Paper Chromatography Introduction Paper Chromatography one method for testing the purity of compounds and identifying substances. This is a useful technique because it is relatively quick and requires small quantities of material. Separations in paper chromatography involve the same principles as those in thin layer chromatography. The substances are distributed between a stationary phase and a mobile phase. The stationary phase is...
    588 Words | 3 Pages
  • Paper Chromatography - 1569 Words
    EXPERIMENT 5: CHROMATOGRAPHY Abstract Paper Chromatography uses a chromatogram paper as its stationary phase and the solvent as the mobile phase. Retention factor is the ratio of the distance travelled by the sample to the distance travelled by the solvent. This experiment aims to separate organic compounds, to compute Rf values and to identify unknown compounds using Rf values. The ten samples underwent paper chromatography to determine the components of the unknown sample. The mobile phase...
    1,569 Words | 7 Pages
  • Paper Chromatography - 615 Words
    Paper Chromatography Chromatography comes from the Greek word khromatos (color) and graphein (to write); i.e. paper chromatography is literally color writing on paper. This method tests the purity of compounds and identifies substances. This analytical process, despite having been replaced by the success of thin layer chromatography, still stands as a valuable teaching tool, and is nevertheless very common. This method is very useful because not only is it a relatively quick process, but...
    615 Words | 2 Pages
  • Candy Chromatography - 452 Words
    Candy chromatography is a method of analysis of separating and comparing of dyes used in candies and food coloring. It is also a proven method to analyze candy and its complex mixture, without having to taste it. These complex mixtures can be separated back into pure substances. To find out the ingredients of a favorite candy, chromatography would help to define every ingredient found in the candy sample. The word chromatography is derived from the two Greek words, "chroma" meaning...
    452 Words | 2 Pages
  • column chromatography - 638 Words
     Column Chromatography of Plant Pigments Sopheea Celine Pizarras*, Anne Morielle Ponciano*, Emerson Quimba*, and Aimee Reyes* College Of Science, University of Santo Tomas, España blvd., Manila Abstract: Column Chromatography is a solid-liquid adsorption chromatography. It depends on the essential principles so as in thin layer chromatography. This separated and analysed the different components of Capsicum frutescens, siling labuyo with the use of reagents such as Hexane, Dichloromethane,...
    638 Words | 3 Pages
  • Chromatography of Pigments - 665 Words
    Introduction Paper chromatography is a method using a chromatography paper to identify the many mixtures of one simple color. The word chromatography comes from the Greek words, "chroma," meaning color, and "grafein," meaning to write. The process of this experiment is to place a colored dot, such as marker ink, onto the chromatography paper, one centimeter away from the triangular tip. Next dip your chromatography paper into the solvent, not allowing it to touch the colored dot. Let it sit...
    665 Words | 3 Pages
  • Column Chromatography - 1301 Words
    Column Chromatography of Plant Pigments Jaybee Balilea, Sharmaine Baysic, Maria Anjelette Patricia Belen 3Bio-7, Department of Biological Sciences, University of Santo Tomas, Manila, Philippines Abstract Column Chromatography is a form of solid-liquid adsorption chromatography and depends on the essential principles as does in thin layer chromatography. It was used in this experiment in separating and analyzing the different components of Capsicum frutescens (siling labuyo) with the use...
    1,301 Words | 5 Pages
  • Chromatography of Commercial Analgesics - 890 Words
    Chromatography of Commercial Analgesics Using the thin layer chromatography the major components of a commercial analgesic tablet will be indentified and purified. The active ingredients of the analgesic used were characterized by comparison with chromatograms of aspirin, acetaminophen, ibuprofen, mefanemic acid, and caffeine through the use of UV chromatography then the Rf value was calculated for each solvent. INTRODUCTION In this experiment, thin-layer chromatography (TLC) will be...
    890 Words | 4 Pages
  • Tlc and Paper Chromatography - 562 Words
    Thin Layer Chromatography/ Paper Chromatography Lab # 10 11/26 Sample # 32 for TLC Sample # 1 for Paper Introduction: Chromatography is one of the most important separation techniques used in all fields of chemistry ranging from analytical chemists to pharmacists. The understanding of how chromatography works and how to operate instruments used to carry out the procedures is an important lab technique to learn. Experiment: Thin Layer Chromatography. Lab #10-1 Paper...
    562 Words | 3 Pages
  • Chromatography of Food Dye - 905 Words
    MOHAWK COLLEGE OF APPLIED ARTS AND TECHNOLOGY CHEMICAL AND ENVIRONMENTAL TECHNOLOGY DEPARTMENT Lab Report ROOM NO: FE E309 EXPERIMENT NO : 8 TITLE : Thin Layer Chromatography of Food Dyes Submitted by Class Partners Instructor : Lyndsay Grover : BIOL 10000 lab. : Awatif Hagelamin : Farag Soliman Date lab performed : February 3, 2011 Date of submission : February 10, 2011 FENNELL CAMPUS HAMILTON, ONTARIO 1/3 Purpose: The purpose of this experiment was to observe the reactions...
    905 Words | 3 Pages
  • Chromatography Analysis of Black Pens
    Chromatography Analysis of Black Pens to Determine Unknown Sample Purpose: Paper chromatography was performed on five different black pens, using four different solutions to determine which would be most appropriate to use on an unknown sample. Paper chromatography was then performed on the unknown sample to identify which pen was used to create it. Procedure: Each person participating in the analysis was assigned one solution to work with: V. Temple used distilled water, D. Sellers...
    710 Words | 3 Pages
  • Chromatography Lab Repot - 668 Words
    Design and carry out a scientific investigation on any one factor that affects retention on paper chromatography Design and carry out a scientific investigation on any one factor that affects retention on paper chromatography CHEMISTRY LAB REPORT CHEMISTRY LAB REPORT Name: Sri Danielle Class: 9A Teacher: Sir Ajay Name: Sri Danielle Class: 9A Teacher: Sir Ajay Aim My aim for this experiment is to find out how different types of solvent will affect the retention factor in paper...
    668 Words | 4 Pages
  • Candy Chromatography Research Paper
    I. INTRODUCTION A. Background of study Candy-coated sweets come in different colors and sometimes even vary in flavor in association with the colors. These colors provide a creative display for children to easily get attracted to the particular treat, leaving those tinted marks on their mouths and a happy, satisfied grin on their faces. The reason for conducting the research is to know how the colors of our favorite candy-coated sugary rewards came to be and that method of knowing is...
    1,478 Words | 5 Pages
  • CHEM 2204 Chromatography Lab
    CHEM 2204 Chromatography Lab by wyk.wong » Fri Jul 11, 2014 10:25 am Results and Calculations Rf values Rf=(Distance moved by the spot (cm))/(Distance moved by the solvent front (cm)) Toluene: Rf=2 cm/3.8 cm=0.53 (Fluorenone) Rf=1.1 cm/3.8 cm=0.29 (Fluorene) Hexane: Rf=1.8 cm/2.2 cm=0.82 (Fluorene) Rf=0 cm/2.2 cm=0 (Fluorene Table 1: Experimental IR peaks compared to literature IR peaks for fluorenone Functional group Experimental peak (cm-1) Literature peak (cm-1) C-H 3010.5...
    1,263 Words | 5 Pages
  • Chromatography Lab Report - 677 Words
    Chromatography lab Purpose: To separate food colorings into their component dyes using paper chromatography. Materials: Chromatography paper, Food coloring, Ruler, Pencil, Solvent solution, Test tubes, Test tube rack. Safety precaution: wear aprons, to make sure that you don’t get any of the alcohol on your clothes, and if you break a test tube you don’t get glass on you. Procedure: See-attached handout. Results: See chromatography with Audrey’s lab report....
    677 Words | 3 Pages
  • Chromatography of Plant Pigments - 1376 Words
    CHROMATOGRAPHY OF PLANT PIGMENTS Marquez, Ma. Rica Paulene, Moises, Patrisha Kate, Policarpio, Jairus Paolo, Rolda, Zylene Joy Department of Biology, College of Science, University of the Philippines Baguio April 23, 2013 ABSTRACT The objective of this experiment was to apply the technique of paper chromatography as a method for separating individual plant pigments contained in plant tissue extracts containing pigment blends. The process of chromatography separates molecules because of...
    1,376 Words | 5 Pages
  • chromatography lab write up - 1035 Words
     Separation Of A Dye Mixture Using Chromatography ********* AP Chemistry (3rd Block) Fauquier High School 8/ 31/ 13 ABSTRACT: The Rf values of seven known color dyes and three unknown samples were observed in order to compare results and discover the hidden identities of the three samples. Three solvents, H2O, Isopropyl alcohol, and a 2% NaCl solution, were introduced to the dotted chromatography strips to show three different ratios for each dye...
    1,035 Words | 8 Pages
  • Chromatography of Commercial Analgesics - 1868 Words
    Chromatographic Analysis of a Commercial Analgesic’s Active Component I. Abstract This study on Chromatographic Analysis of a Commercial Analgesic’s Active Component seeks to separate, identify, quantify and analyze the active component found in Biogesic®, which may be useful for public awareness despite its wide acceptance and availability in the market. Furthermore, this study applies thin layer chromatography (TLC) technique since it is the simplest, cheapest yet most effective approach...
    1,868 Words | 6 Pages
  • Separation of Ferrocene and Acetylferrocene by Chromatography
    Purpose 1. To prepare a dry-packed sample of product mixture of ferrocene and Acetylferrocene 2. To separate and purify the components in the product mixture by column chromatography. 3. To check the purity of the components by thin-layer chromatography (TLC). 4. To calculate the yield of acetylferrocene and the percent recovery of unreacted ferrocene. Table of Quantity showing various physical properties Type of substance Molecular Formula Molecular Weight (g/mol) Density(g/cm3)...
    1,606 Words | 6 Pages
  • Chromatography: Food Coloring and Food Dyes
    Name ________________________12345_______________________ Date ____23.8.2013___________ Block _______ Training Lab: Paper Chromatography I. Background Information: When working in a lab, scientists often need to identify different molecules that are present in a sample they are studying. There are many ways to identify unknown molecules/chemicals in a sample. The method you will be using today is called Paper Chromatography and consists of 2 steps. First, you will separate the unknown...
    1,133 Words | 4 Pages
  • Chromatography of M&M and Ink Dyes
    Separations: Chromatography of M&M and Ink Dyes Almost all substances we come into contact with on a daily basis are impure; that is, they are mixtures. Similarly, compounds synthesized in the chemical laboratory are rarely produced pure. As a result, a major focus of research in chemistry is designing methods of separating and identifying components of mixtures. Many separation methods rely on physical differences between the components of a mixture. For example, filtration takes advantage of...
    1,942 Words | 8 Pages
  • Separation of Photosynthetic Pigments by Paer Chromatography
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  • Acetylation of Ferrocene 2 - 1069 Words
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  • Cation Unknown Identification Using Transition Metal Complexes
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  • Recrystallisation and Chemical Separations - 857 Words
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    2,297 Words | 10 Pages
  • Recrystallization - 2265 Words
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  • Steam Distillation of Eugenol from Cloves.
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    487 Words | 3 Pages
  • PRACTICAL REPORT ON THE ISOLATION AND IDENTIFICATION OF CODEINE AND PARACETAMOL
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    1,192 Words | 4 Pages
  • Extraction of Chlorophyll from Spinach Leaves
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    1,391 Words | 4 Pages
  • Paper Chromatagraphy - 1756 Words
    Paper Chromatography: Separation of Cations and Dyes 04.12.10 Purpose: To have become acquainted with chromatographic techniques as a method of separation and identification of substances Materials: Evaporating dishes (2) 4 in. watch glasses (2) Paper chromatography strips 12.5 cm Whatman filter paper 600 mL beaker 100 mL graduated cylinder Capillary pipets paper clips (3) 6 in glass stirring rod metric ruler Chemicals: 0.5 M Cu(NO3)2 0.5 M...
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  • Formal Report on Exp 5
    COLUMN AND THIN LAYER CHROMATOGRAPHY Mark Paul P. Pastrana, Mariah Ericka M. Patawaran, Princess Juneire M. Peligro, Francisco Q. Pua III, Rose Anne L. Quyo and Janille P. Ragpa Group 8 2B Medical Technology Organic Chemistry Laboratory ABSTRACT The main objectives were to separate the colored components of malunggay leaves by means of column chromatography, as well as to determine the purity of the components using thin layer chromatography (TLC) and measure the Rf values of the...
    1,421 Words | 6 Pages
  • Tlc questions - 567 Words
    Post lab Questions 1. 3-decanone will have a higher Rf value than 3-decanol, toluene will have a higher Rf value than benzoic acid, and cyclooctane will have a higher Rf value than cycloctanone. This is because they are not as polar as the compounds with lower Rf values, the compounds that are most polar tend to stay longer with the polar adsorbent, while the least polar compound travels with the eluent that is lower in polarity. 2. TLC in today’s experiment stands for Thin Layer...
    567 Words | 2 Pages
  • Gradient Elution of Plan Components (Hexane-Soluble Extract)
    Column Chromatography ________________________________________________ You have already performed two chromatography experiments: gas chromatography and thin layer chromatography. All chromatography experiments involve passing a mixture of analytes through a system that includes a mobile phase and a stationary phase. The partitioning of the analytes between these two phases determines the rate at which they pass through the system, and (in theory) allows them to be separated from one another....
    1,887 Words | 6 Pages
  • Micro-extraction of urine and/or stomach contents and analysis by TLC
    Micro-extraction of urine and/or stomach contents and analysis by TLC Abstract Thin Layer Chromatography (TLC) is one of the oldest chromatographic techniques used for the identification of compounds and for determination of the presence of trace impurities. The aim of the experiment was to determine two unknown samples (X and Y) by comparing results to the known standard drugs, of which there was five, using a specific TLC method. The same technique was used for the determination...
    1,351 Words | 6 Pages
  • Tlc Chromothography - 2671 Words
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    2,671 Words | 11 Pages
  • Tlc Full Lab Report
    TLC FULL LAB REPORT Objective: The purpose of this experiment was to identify compounds in a mixture by Rf values and to determine the best solvent to use. Also is the analysis of mixtures before, during and separation. Possible solvents: Hexane MW- 86.17 g/mol Hazards-flammable, harmful if swallowed Melting pt / boiling pt (degree Celsius) - 69 Density-.6548 g/ml [pic] Methanol MW-32.04 g/mol Hazards- flammable, eye irritant Melting pt / boiling pt- 65 Density-.7918 g/ml...
    895 Words | 4 Pages
  • Experiment 1: Mechanism of Nitrile Oxide and Alkene Cycloaddition
    Experiment 1: Mechanism of Nitrile Oxide and Alkene Cycloaddition 1. Purpose: to clarify the mechanism for the cycloaddition reaction between benzonitrile oxide and an alkene, and to test the regiochemistry of the reaction between benzonitrile oxide and styrene. 2. Plan: Each student in a group of three will work to create a reaction with the Benzonitrile Oxide with, cis-stilbene, trans-stilbene, or styrene in an Erlenmyer flask. With this Reaction solution thin layer chromatography will...
    2,983 Words | 9 Pages

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