Molecular biology Essays & Research Papers

Best Molecular biology Essays

  • Molecular Biology - 530 Words
    Molecular Biology I Pre-lab Assignment: Before completing this assignment, read the Molecular Biology I and II Lab Exercise posted on CourseWeb. You should not re-type the questions, but the answers must be typed and written in complete sentences. You will turn in a hard copy to your instructor and submit an electronic copy to using the enrollment password pittbio2013 and the class ID provided by your instructor. Directions for submitting an assignment to are...
    530 Words | 2 Pages
  • Sop on Molecular Biology - 832 Words
    Statement of Purpose Some people view biological science as an area of science which is boring; requiring intensive memorization and less logical reasoning. True to my heart, my view towards biological science was no difference! It was not before my higher secondary school classes when I first realized the beauty of this scientific arena. I had my first glimpse at molecular biology then. But that brief introduction to molecular biology was enough to compel me to the idea that I want to spend my...
    832 Words | 2 Pages
  • Molecular Biology and Ldh - 6183 Words
    *Running title: Isolation and Characterization of Avian Lactate Dehydrogenase To whom correspondence should be addressed Sylvia DaoudKinze and James Proestes, Department of Biochemistry, Portland State University Professor, Portland Oregon, 97207-0751; E-mail: and ------------------------------------------------- Keywords: Lactate, Dehydrogenase, Avian, Bradford Assay, Affinity Column Background: Lactate Dehydrogenase also known as LDH is an...
    6,183 Words | 21 Pages
  • Genetics and Molecular Biology - 452 Words
    [pic] Faculty of Science Undergraduate Modular Scheme Session 2010/2011 Semester 2 Level 4 MODULE: LS1180: Genetics and Molecular Biology DATE: 31 May 2011 TIME: 09.30 – 11.30 Instructions to Candidates This paper contains THIRTY-EIGHT questions in FIVE sections: Sections A, B, C, D and E. Answer THIRTY-FOUR questions only Section A: THIS SECTION HAS BEEN WITHHELD BY THE FACULTY Answer ALL THIRTY questions on the MCQ sheet provided Section...
    452 Words | 4 Pages
  • All Molecular biology Essays

  • Molecular Biology Ii - 1260 Words
    NAME:__________________________________ STUDENT#:______________________________ YORK UNIVERSITY FACULTY OF SCIENCE AND ENGINEERING SC/BIOL 3130N (3.0) – MOLECULAR BIOLOGY II MIDTERM I – January 31st, 2012, VERSION A INSTRUCTIONS: 1) Please sign the sign-in sheet that will be circulated during the exam. 2) Multiple choice – 12 questions, one mark each. Choose the best single mostcorrect answer and fill in all answers on the scantron sheet provided. Questions where more than one answer is selected...
    1,260 Words | 4 Pages
  • Statement of Purpose for Molecular and Cellular Biology
    Statement of Purpose Applicant�s name: xx It is noticed that science develops in splashes, each significantly advancing our understanding of the Nature. The 19th century was the time of rapid development of thermodynamics and electrodynamics; in the beginning of the 20th century quantum mechanics and relativity theory were born. In the search for secrets of nature science has reached extremely high and low energies as well as large and small length scales. Still until recently we knew...
    714 Words | 3 Pages
  • Molecular Biology and W. H. Freeman
    CHAPTER 14 LECTURE NOTES: RECOMBINANT DNA TECHNOLOGY I. General Info A. Landmarks in modern genetics 1. Rediscovery of Mendel’s work 2. Chromosomal theory of inheritance 3. DNA as the genetic material 4. Recombinant DNA technology development and applications B. Recombinant DNA refers to the creation of new combinations of DNA segments that are not found together in nature. The isolation and manipulation of genes allows for more precise genetic analysis as well as practical...
    2,177 Words | 8 Pages
  • Biology - 696 Words
    1. Outline the process of DNA profiling (genetic fingerprinting), including ways in which it can be used. 6 marks • sample of DNA obtained / leucocytes / from mouthwash / hair / other named source • satellite DNA / repetitive sequences used for profiling • amplification of DNA by polymerase chain reaction / PCR • cutting DNA into fragments using restriction enzymes • separation of fragments of DNA (by electrophoresis) • separation according to the length of the fragments • pattern...
    696 Words | 3 Pages
  • Molecular Biotech - 431 Words
    MOLECULAR FARMING INTRODUCTION Biotechnology in agriculture has two categories: 1. “Improvements” to existing livestock and crops, and 2. Development of entirely new uses for both animals and plants (biopharming). “Improvements", include ‘input traits’ such as crops with extra resistance to insect attack, improved weed control, increase the plants tolerance to cold, drought and other environmental factor. Ex "Roundup ready" soya, "Starlite" corn, or "Frost-tolerant" tomatoes. WHY...
    431 Words | 2 Pages
  • Biology Lab - 2372 Words
    IB SL Biology Lab Molecular Biology: Transformation and Electrophoresis Christina Qi 2/16/07 Aim: How can a plasmid be engineered to include a foreign piece of DNA and how does gel electrophoresis separate DNA molecules present in a mixture? Hypothesis: If the pGLO plasmid is inserted into competent Escherichia coli cells, then the transformed bacteria will be resistant to ampicillin and will glow green under UV light. If samples of DNA are cut using certain...
    2,372 Words | 8 Pages
  • Molecular Biologist's Guide - 1309 Words
    Molecular Biology 344 fall 2012 A Johnson Due 9/11/12 as hard copy at the beginning of lecture Problem Set 1 1. Look carefully at the structures of the two molecules shown below. a. What would you expect to happen if you added ddCTP (shown in a) to a DNA synthesis reaction in vitro in large excess over the concentration of deoxycytidine triphosphate (dCTP)? How will this affect the pattern of bands on the sequencing gel? The ddCTP lacks the OH, which is required for...
    1,309 Words | 4 Pages
  • Report on the Connection Between the Central Dogma of Molecular Biology/ Bioinformatics, Model Organism and Drug Designing.
    Report on the connection between the Central dogma of Molecular Biology/ Bioinformatics, Model Organism and Drug Designing. The basis of the central dogma of molecular biology is the expression of the genetic information in any call. It is a universal process that occurs in every cell. The genetic information is stored in the DNA. During gene expression DNA is transcript to RNA and these RNA are transcribed to proteins. Bioinformatics deals with the genetic information which involves...
    414 Words | 2 Pages
  • Molecular Biologist Career Journal
    MOLECULAR BIOLOGIST Education/Training Required: All molecular biologists must at least earn a bachelor’s degree in a related science field. Required courses usually include cell biology, evolutionary biology, genetics, ecology, general chemistry, biochemistry, organic chemistry, physics, molecular biology, multivariable calculus, and linear algebra. Most jobs for molecular biologists require a doctoral degree. During college, students may want to participate in science-related...
    467 Words | 2 Pages
  • The Emerging Molecular Diagnostics Industry:
    Kellogg School of Management The Emerging Molecular Diagnostics Industry: Applera’s Path to a Leadership Role June 5, 2003 By John Fadlovich Carl Finamore Jodi Flax Kevin Lynch Rebecca Wildman Applera Corporation in the Molecular Diagnostics Industry Table of Contents 1. 2. Introduction................................................................................................................. 1 Industry Overview...
    6,535 Words | 25 Pages
  • Molecular detection of fungal pathogens.
    MOLECULAR DETECTION OF FUNGAL DISEASES INTRODUCTION With the rise of many new diseases caused due to viruses, bacteria and fungi; it is essential for the rapid detection of such diseases. The severity of such diseases can be reduced by its rapid detection carried out by different methods. The conventional methods may include the idea of just identifying the disease symptoms, identification of these pathogens in the laboratory by different morphological and biochemical tests, etc [1]. The...
    1,440 Words | 5 Pages
  • Amateur Hobbyists Are Creating Home-Brew Molecular-Biology Labs, but Can They Ferment a Revolution?
    Amateur hobbyists are creating home-brew molecular-biology labs, but can they ferment a revolution? $195–$1,000 $250–$2,000 MICROCENTRIFUGE $60–$850 IMPROVISATION IS KEY To do molecular biology on the cheap, biohackers have developed some creative workarounds: • for a $10 microscope, pop the lens o a webcam and stick it back on backwards. • for an $80 centrifuge, order the DremelFuge rotor and attach to a Dremel rotary tool. • for a free 37 ºC incubator, incubate tubes of E. coli...
    1,979 Words | 6 Pages
  • Biology Prac Report Example
    Aim: The aim of this experiment is to investigate the process of Electrophoresis and successfully analyse DNA fragments. Hypothesis: That the experiment will show the visual representation of DNA proteins, and that the shorter the band the further is will travel. Background: Restriction enzymes are DNA-cutting enzymes found in bacteria, which cut DNA into smaller fragments. A restriction enzyme recognizes and cuts DNA only at a particular sequence of nucleotides, known as restriction...
    1,259 Words | 5 Pages
  • cell biology exam1 - 2922 Words
    The objective of the experiment was to differentiate mammalian C2C12 cells into muscle cells and analyze for presence of myosin light chain. Utilizing Western Blotting techniques and using specific antibodies helped to detect myosin light chain. The hypothesis was that proliferating myoblasts could be induced to initiate the differentiation process by depriving the cells of the necessary growth factors and that the differentiation is characterized by the expression of muscle-specific...
    2,922 Words | 9 Pages
  • Biology and Words Biophysics Acts
    Biophysics With growing technologies our society is facing various physical and biological problems of global proportions. Will we be able to feed the world’s large population? How will we stop global warming? Will we be able to preserve the biological diversity? How do we save clean and plentiful water which is our life ? So, here is Biophysics for all of us to provide technologies for meeting these challenges, based on the mechanisms of biology and the principles of physics. Biology studies...
    438 Words | 2 Pages
  • Study Guide for Biology - 731 Words
    BIOLOGY 1201, Section #2 Spring, 2014
(MWF 9:30-10:20 AM) Instructor: Dr. Terry M. Bricker
Office: A606 Life Science Annex (Laboratory, A623 – check the lab if I am not in the office!) Phone: 225-578-1555
E-mail: -- E-mail is an excellent way to contact me or ask questions.
Office Hours: M,Tu 3:30-4:30, or by appointment. - Please see me if you have questions or problems. COURSE: BIOL 1201, General Biology, 3 credit hours. Biology 1201 is a course intended for students that...
    731 Words | 3 Pages
  • Biology Case Study - 732 Words
    Advanced Cell Biology II Step 1: How will you identify the “vital” cellular protein that the virus targets for degradation? (Hint: think proteomics). (3 pts.) First, since we know the viral RNA sequence and its targeting protein, we can investigate it in bioinformatics database, and can acquire some clues or hints about the target proteins. From the bioinformatics database, we might be able to find its structure, similarity with other proteins, functions, and binding domains. In other...
    732 Words | 3 Pages
  • Molecular Genetics: Catching the Criminal Using Electrophoresis
    Introduction A sample of DNA found in a crime scene was provided along with five suspects. Their DNA was then processed using restriction enzymes and Agarose Gel Electrophoresis. The objective of this lab was to match a criminals DNA to a crime scene using restriction enzymes EcoRI and Pstl with Agarose gel electrophoresis. Restriction enzymes cut DNA at a specific base pair site recognized by the enzyme, which then turns one single strand of DNA into many fragmented strands of DNA. EcoRI...
    866 Words | 3 Pages
  • Ap Biology Chapters 20 and 21
    Calvin Williams Chapter 20 1. Advances in recombinant DNA technology allow scientists to work with smaller fragments of DNA, give them more tools to dissect and analyze DNA, and also allow for them to make many copies of a strand of DNA. 2. Restriction enzymes are made by bacteria to cut up invading DNA. They target specific base sequences in the DNA and then work to cut out those sequences from the DNA. 3. When a restriction enzyme cuts out a portion of DNA, it will sometimes leave a...
    1,001 Words | 3 Pages
  • Biology 001 Lab Report 2
     Separation of Proteins and Mass Analysis Using SDS PAGE Biology 00-01L Abstract This experiment consisted of separating proteins into polypeptides using a method called SDS PAGE which is a type of electrophoresis. The polypeptides had different masses, so each polypeptide traveled a different distance and this was an essential part of the lab which demonstrated that there exists a relationship between the distance traveled by the protein and the mass...
    2,721 Words | 8 Pages
  • AP Biology pGLO Lab Write Up
    Josh P. AP Biology 12-10-13 Lab Writeup The Effect of Transformation of pGLO in Bacteria Background/Objectives/Hypothesis: Genetic transformation is a process that primarily is inserting new DNA into an organism to change that organism’s trait. This process has many useful benefits when used correctly in different organisms. In this lab, bacteria was transformed by inserting DNA for Green Fluorescent Proteins. The DNA for these proteins were taken from bioluminescent jellyfish...
    938 Words | 3 Pages
  • Application of Molecular Techniques for Detection of Disease Resistant Genes in Tomato Breeding Lines for Guatemala
    Application of Molecular Techniques for Detection of Disease Resistant Genes in Tomato Breeding Lines for Guatemala Objectives: 1. Evaluate and modify methods for detection of Fusarium Race 2 resistance gene. 2. Evaluation of two step protocol for detection of Mi-1 gene. 3. Verification of marker for Ty-1 and evaluation for marker in Guatemala breeding lines. (This is a Geminivirus that is transmitted by the whitefly) 4. Use of molecular markers to detect geminivirus resistance source for...
    1,432 Words | 5 Pages
  • Molecular Phylogeny of the Endangered Vietnamese Paphiopedilum Species Based on the Internal Transcribed Spacer of the Nuclear Ribosomal Dna
    Advanced Studies in Biology, Vol. 5, 2013, no. 7, 337 - 346 HIKARI Ltd, Molecular Phylogeny of the Endangered Vietnamese Paphiopedilum Species Based on the Internal Transcribed Spacer of the Nuclear Ribosomal DNA K. H. Trung1, T. D. Khanh2 , L. H. Ham2, T. D. Duong2 and N. T. Khoa2 1 2 Genetic Engineering Lab, Agricultural Genetics Institute, Hanoi, Vietnam Molecular Biology Division, Agricultural Genetics Institute, Hanoi,...
    299 Words | 1 Page
  • Biomed Personal Statement - 481 Words
    |Personal statement | | I have always found science interesting since I was a kid and knowing that it is the discoveries in this area that make the evolution and progression of man as a race to advance at a rapid rate has made me develop a strong wish to learn more about the concepts of life, the processes and factors that lead to certain changes in our bodies at a molecular level. This led me into wanting a degree in biomedical science...
    481 Words | 2 Pages
  • comparative proteomics - 953 Words
    Comparative Proteomics: Protein Profiler Lab by Jonathan Thulson Biology 113 October 6, 2013 Lab Partner: Vernon Morris INTRODUCTION Proteomics is the study of proteins. Their functions, interactions with other proteins, cellular locations and levels at which they are expressed. The purpose of this lab was to compare the proteins present in different species of fish to be able to determine which species of fish have the closest relation. This can be determined based on...
    953 Words | 4 Pages
  • Colon Cancer Report - 889 Words
    Colon Cancer Scenario Introduction: Gel electrophoresis is an important molecular biology tool: it enables us to study DNA. It can be used to determine the sequence of nitrogen bases, the size of an insertion or deletion, or the presence of a point mutation; it can also be used to distinguish between variable sized alleles at a single locus and to assess the quality and quantity of DNA present in a sample. Gel electrophoresis is a method of separating chemical compounds and molecules by their...
    889 Words | 3 Pages
  • Gel Electrophoresis - 491 Words
    Restriction Enzyme Analysis of DNA Abstract: This lab is about using gel electrophoresis to compare the DNA samples of 4 suspects to DNA found at a crime scene. Gel Electrophoresis sorts DNA by length or charge. 6 viles of DNA have been put through PCR will have restriction enzymes mixed in. A Jell-O like substance from Argose and a liquid buffer is combined and placed in a mold with a gel comb making little wells, then refrigerated until it is formed into a gel. The gel is placed in a...
    491 Words | 2 Pages
  • Its a fun paper - 365 Words
     Lab 12: RFLP Analysis Using Gel Electrophoresis Rahul Truter Jordan Higley Purpose Statement: To see the uses of Gel electrophoresis and how the RFLP analysis can be used in legal justice. Principles: 1. Define the following terms: a. An enzyme that cuts DNA at certain base patterns b. A process that separates fragments of DNA by length and charge c. The fragments of DNA after a Restriction Enzyme is used on a strand of DNA 2. Ecor1 5' GAATTC 3 Bam HI GATCC 3. They are separated...
    365 Words | 2 Pages
  • Career Journal on Biochemist - 404 Words
    Kris Lukas Career Journal Name: Biochemist Education and Training -Candidates should have a high school diploma with good grades in chemistry and biology -A Bachelor’s degree in chemistry is good to have as well as a Doctoral degree -Good to have a Major’s degree in biology, chemistry, or biochemistry - Should have between 18-24 months of experience working as a biochemist in a research facility or as an assistant Responsibilities -A typical work day includes...
    404 Words | 2 Pages
  • Extraction of Plasmid Dna - 940 Words
    Isolation of plasmid DNA and analysis of isolated plasmid Introduction: A plasmid is an autonomously replicating extra-chromosomal genetic element. In other words, this is a DNA molecule external to the bacterial chromosome that is able to replicate on its own and distribute its daughter molecules to daughter cells. You have successfully cloned a fragment of chromosomal DNA containing a tetracycline resistance cassette into a plasmid (pET11a). To this end you have (1) isolated total...
    940 Words | 3 Pages
  • Cell Bio - 1023 Words
    Abstract Western blotting is an analytic technique used around the world for many reasons; detecting infections, diseases, and particular proteins in a tissue. When western blotting, first the proteins are extracted and undergo Gel Electrophoresis, next it goes through elecroblotting, and finally detects the proteins. When the results after every step is completed, the membrane is analyzed based on where the bands are present in the gel. It has been told that actin and myosin is present in...
    1,023 Words | 3 Pages
  • Pcmb Books - 387 Words
    Physics: 1. Science For Everyone By SS Krotov—Aptitude Test Problems In Physics. 2. 2000 Puzzling Physics Problems With Hints And Solutions By Peter Gnadig, Gyula Honyek And Ken Riley. Cambridge University Press 3. Exercises In Introductory Physics By R.B. Leighton And R.E.Vogt California Institute Of Technology. Addison-Wesley Publishing Company, Reading Massachusetts. 4. University Physics By Freedman And Young, 11th Edition Onwards. 5. Foundations Of Electromagnetic Theory By...
    387 Words | 2 Pages
  • Individual Report on Gel Electrophoresis
    Individual report on gel electrophoresis Date: 22/9/2011 Aim: To find the the suspect of the crime by comparing the DNA bands from the two suspects, the victim and the evidence. Results: Suspect 1 Suspect 2 Victim Evidence Suspect 1 Suspect 2 Victim Evidence Wells DNA band Agarose gel (immersed in buffer solution during electrophoresis Wells DNA band Agarose gel (immersed in buffer solution during electrophoresis Deduction: Suspect 2 is excluded as the source the...
    250 Words | 2 Pages
  • Gel Electrophoresis Lab Report
    Gel Electrophoresis Adventure Intro The final goal of this lab was to successfully measure the size of different samples of DNA by placing each sample into a well in agarose gel and running a current through a charged chamber. The DNA samples will move through the gel towards the positive charge. Ideally, the DNA will move and create and sequence of smallest to largest. This lab exposes us to DNA technology. Backround ...
    691 Words | 1 Page
  • Extraction and Analysis of Plasmid Dna from E. Coli Cells
    Extraction and Analysis of Plasmid DNA from E. coli Cells Introduction A plasmid is an extra-chromosomal element, often a circular DNA. Since a plasmid is by definition an extra-chromosomal element, it cannot make use of any origin of DNA replication in a chromosome (BP site). Meaning that DNA synthesis within a plasmid depends on having an origin of DNA synthesis of its own. Plasmids are often found in bacterial cells, in which they are used as transfer agents for transmitting various...
    2,774 Words | 9 Pages
  • 1.3.1 response - 620 Words
     Activity 1.3.1: Student Response Sheet PART A- Restriction Enzymes Restriction enzymes are a tool that allows us to pinpoint human identity down to single differences in our DNA. Work through the following simulation so you can see these molecular scissors in action. Find out more about restriction enzymes by viewing the animation and reading the article listed below. DolanDNALearningCenter: Restriction Enzymes Access...
    620 Words | 4 Pages
  • Restriction Enzymes - 827 Words
    Kenneth Hampton | | |Restriction Enzymes: | |A study in Reactions and Mapping | | | |November 7, 2008 | ABSTRACT This experiment will study...
    827 Words | 4 Pages
  • Gel Electrophoresis - 966 Words
    Alexander Davis Period: 1 Group Partners: Jenny Case Blake Rebek Matthew Heigm...
    966 Words | 4 Pages
  • Statement of Purpose - 453 Words
    STATEMENT OF PURPOSE MS Biotechnology I have been dedicated towards my studies right from childhood. During my school days onwards I developed a special flair towards biology and it is that interest that has persuaded me to take Biology as one of the majors in my 12th grade and Biotechnology as the major in my under graduation. During my under graduation...
    453 Words | 2 Pages
  • Gel Filtration - 2053 Words
    Gel filtration GEL FILTRATION OF PROTIENS Aim: The aim of this experiment is to identify proteins from a complex mixture using the gel filtration technique also known as size exclusion chromatography. This technique is widely used by biochemists when proteins larger than the pores are excluded from the column and the smaller molecules elute last and then collected in test tubes for examination by spectroscopic techniques. The red/brown proteins, in particular, will be observed...
    2,053 Words | 7 Pages
  • Biochemistry Report - 984 Words
    Restriction Endonuclease Digestion of DNA from E. coli cells and Analysis by Agarose Gel Electrophoresis Introduction The main goals of this experiment are testing an alternative procedure called “boiling lysis”, evaluating the quality of the purified plasmid for restriction digests, and identifying the mislabeled plasmid. The plasmid DNA from a carrier E. coli strain was purified by the boiling lysis. In the boiling lysis method, the bacterial cells were given momentary heat...
    984 Words | 5 Pages
  • ganga - 517 Words
    RESUME MOUSUMI GARAI CORRESPONDING ADDRESS A-32, Subhash Pally, Co-op Society Durgapur, Burdwan, West Bengal,...
    517 Words | 5 Pages
  • Gel Electrophoresis Lab - 658 Words
    Gel Electrophoresis Lab SBI4U1 May 13th, 2013 Gel Electrophoresis Lab Purpose: The purpose of this lab is to learn how restriction enzymes cut DNA molecules at specific sequences, thus producing DNA fragments of various lengths. Students learn how fragments form unique patterns, which help to distinguish the base for DNA identification. This lab answers the question “whose DNA was left behind?”. Materials: * Transfer pipets * Agarose Gel * Dyed DNA samples...
    658 Words | 4 Pages
  • Biotechnology - 2741 Words
    e incorporated a fusion tag prior to our genes of interest and attached the expressed fusion proteins covalently on microarrays. This enhances the specific binding of the proteins compared to nitrocellulose. Thus, it helps to reduce the number of false positives significantly. It enables us to screen for immunogenic proteins in a shorter The correct insert size was determined by Colony PCR. Plasmids from clones containing the correct-sized inserts were isolated and the MCS was sequenced using...
    2,741 Words | 9 Pages
  • Restriction Enzyme Lab Report
    I. Title. Restriction Enzyme Mapping of pBR322 Using Agarose Gel Electrophoresis. II. Authors. Author: Partner: Section: Thursday, 1:10 pm Date of Experiment: October 25, 2012 III. Introduction. Restriction enzymes (or restriction endonucleases), originally isolated from Haemophilus influenzae in 1970, are enzymes within a cell that cleave foreign DNA within a specific and predictable nucleotide sequence (known as a restriction site) regardless of the source of such DNA. Such...
    2,114 Words | 9 Pages
  • Extraction of Bacterial Plasmid Dna and Analysis of Extracted Dna Samples
    Experiment 2 Titles Extraction of Bacterial Plasmid DNA and Analysis of Extracted DNA Samples Objectives * To learn the procedures needed in extracting the bacterial plasmid DNA * To determine the concentration of original DNA sample and purity of prepared DNA sample by using spectrophotometer * To analyze the extracted DNA sample by gel electrophoresis Materials and methods (Refer to UDBB2144 Laboratory 2A Manual Principles of biotechnology page 6-10) Results...
    457 Words | 2 Pages
  • Lab Repotrt - 1640 Words
    Title: Comparing fish protein Introduction: Darwin's Theory of Evolution is the commonly thought notion that all life is linked and has descended from a common ancestor. Darwin's general theory assumes the development of life from nonlife and stresses a purely naturalistic "descent with modification". That is, complex creatures evolve from more simplistic ancestors naturally over time. “Natural selection is a process in which individuals that have certain inherited traits tend to survive and...
    1,640 Words | 7 Pages
  • Generation of Erp60 Knockout Mice
    Introduction: 1 Vitamin D is critical for embryonic skeletal formation and long bone growth. Without vitamin D, the hypertrophic cell zone will expand because the cartilage fails to calcify leading to rickets. Protein disulfide isomerase A3 (PDIA3), also known as ERp60, ERp57, Grp58, and 1,25-MARRS, is a multifunctional protein that has been associated with rapid membrane-initiated signaling by 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3) in several cell types. Knockdown of PDIA3 in...
    2,296 Words | 7 Pages
  • Plant Biotechnology Question Papers
    Plant Biotechnology Long questions * What is in vitro propagation? How can we produce virus-free/pre-basic seed potatoes by thermo-therapy and meristem culture? what is micro propagation? Describe with illustration the protocol of DAS-ELISA. X2 * Define germplam and cryo-preservation (x2). Describe the steps involved in cryopreservation of shoot tip as a germplasm. X3 * Describe the process of T-DNA transfer and its integration in plant cell by the infection of Agrobacterium. X2...
    581 Words | 3 Pages
  • Goings of hucaberry - 1139 Words
    Biochemists are scientists trained in biochemistry. Typical biochemists study chemical processes and chemical transformations in living organisms, other biochemists study DNAs, proteins and cell parts. The word "biochemist" is a portmanteau of "biological chemist". Biochemists also research how certain chemical reactions happen in cells and tissues and observe and record the effects of products as in food additives and medicines. The main function of a biochemist is to focus on improving the...
    1,139 Words | 6 Pages
  • Subcloning Of Fungal CDNA From PBK Lab
    Subcloning of fungal cDNA from pBK-CMW into a plasmid vector pUC19 using fungal gene CIH Introduction A plasmid is a circular, double stranded DNA molecule that replicates independently of the chromosome DNA within a cell.pUC19 is one of the most commonly plasmid cloning vector used due to its high copy replication number (approx. 100 copies per cell), ampR (ampicillin resistance gene) andterminal fragment of β -galactosidase (lac Z). It is circular double stranded and it has 2686...
    1,878 Words | 27 Pages
  • SDS-PAGE - 274 Words
    Electrophoresis of Proteins from Cauliflower Fractions by SDS-PAGE Laboratory Summary Electrophoresis is a technique where molecules are separated according to their physical properties such as size, charge, and/or shape. Charged proteins are commonly separated in this matter using PAGE (polyacrylamide gel electrophoresis) to identify individual proteins present in samples. In this lab, SDS-PAGE was used. SDS-PAGE separates charged proteins primarily by size because the ionic detergent sodium...
    274 Words | 1 Page
  • Professor - 4270 Words
    Characterization of a Yam Class IV Chitinase Produced by the Recombinant Pichia pastoris X-33 Department of Botany, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh Abbreviations: Endo H, Endoglycosidase H; PR protein, pathogenesis related protein; P. pastoris, Pichia pastoris; VTS, vacuolar targeting signal; PCR, polymerase chain reaction; PAGE, polyacrylamide gel electrophoresis; SDS, sodium dodecyl sulfate Abstract A yam (Dioscorea opposita Thunb) class IV chitinase, whose...
    4,270 Words | 13 Pages
  • Analysis of DNA Using Restriction Enzyme Digestion
    Objective: DNA is analyzed by agarose gel electrophoresis after being digested with EcoRI restriction endonucleasse. Procedures: λ DNA and puC18 DNA were put into two tubes respectively. Then, EcoRI buffer, EcoRI enzyme and deionized water would be put into both tubes. EcoRI enzyme was the restriction enzyme that cut the DNA at the specific sequence. The EcoRI buffer enhanced the stability of many enzymes and binds contaminants that may be present in DNA preparations. DI water was used...
    463 Words | 2 Pages
  • Unknown Bacteria - 445 Words
    Identifying Unknown Bacteria Using Biochemical and Molecular Methods Beginning of Instructor Pages Instructor Pages - - 3 Purpose The purpose of this lab is to introduce a variety of lab techniques to students working on the common problem of identifying an unknown bacterium. This lab helps students develop an understanding of the biochemical and molecular differences in bacteria and introduces the concept of identifying species based on characeristic gene sequences. Students work...
    445 Words | 2 Pages
  • Gel Electrophersis - 602 Words
    2. Steps for Southern Blotting • Digest the DNA with an appropriate restriction enzyme. • Run the digest on an agarose gel. • Denature the DNA, which would separate double-stranded DNA into single-stranded DNA. • Transfer the denatured DNA to the membrane. • Probe the membrane. • Visualize your radioactively labeled target sequence. If you used a radiolabeled 32P probe, then you would visualize by autoradiograph. 3. a) She placed the nucleotide as she did because the EcoR I cuts in...
    602 Words | 3 Pages
  • Western Blotting - 1563 Words
    Western Blotting is used to Identify a Subunit of Myosin Light Chain in the Proteins of All the Different Fish. Chae Yee Kent State University: BSCI 30140 Lab Abstract Western Blotting can be used to detect the Myosin actin light chain in different species of fish and is used to distinguish from different species based on variation, commonality, or evolutionary divergence. First, proteins are extracted from the tissue and loaded into a gel matrix. The matrix will separate the proteins...
    1,563 Words | 5 Pages
  • Shotgun Sequencing - 3383 Words
    POJ 4(4):195-198 (2011) ISSN:1836-3644 Research Note Comparative evaluation of PCR success with universal primers of maturase K (matK) and ribulose-1, 5-bisphosphate carboxylase oxygenase large subunit (rbcL) for barcoding of some arid plants Sameera O. Bafeel1, Ibrahim A. Arif2,3, Mohammad A. Bakir2,3, Haseeb A. Khan2,3*, Ahmad H. Al Farhan2,3, Ali A. Al Homaidan2,3, Anis Ahamed2,3, Jacob Thomas3 1 2 Department of Biology, College of Science, King Abdulaziz University, Jeddah, Saudi...
    3,383 Words | 10 Pages
  • Gel Electrophoresis of Extracted Dna 0.5% Agarose Gel
    820 Words | 3 Pages
  • SDS-PAGE - 1268 Words
    When electrophoresis is done, proteins in a sample can be quantitated and analyzed. The separation of macromolecules in an electric field is called electrophoresis. A very common method for separating proteins by electrophoresis uses a discontinuous polyacrylamide gel as a support medium and sodium dodecyl sulfate (SDS) to denature the proteins. The method is called sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) where one can obtain information about the size of a protein...
    1,268 Words | 4 Pages
  • nooo - 559 Words
    Study Guide for the BME5 Midterm The midterm will cover information from lectures 1-4. Subject areas that are fair game for the test are listed below. I may not use every single item in the exam. Note that the level of detail required is that which was covered in lecture. Only information covered in lecture will be on the tests (includes info in figures and tables). Lecture 1 Processes that utilize recombinant DNA technology (and molecular processes that don’t) Basic science; what it...
    559 Words | 3 Pages
  • Dna Digestion and Electrophoresis - 712 Words
    DNA DIGESTION AND ELECTROPHORESIS In this experiment we will be doing a process called as DNA digestion or also known as restriction digest. A restriction digest is a procedure used in molecular biology to prepare DNA for analysis or other processing. It is sometimes termed DNA fragmentation, scientists Hartl and Jones describe it this way: This enzymatic technique can be used for cleaving DNA molecules at specific sites, ensuring that all DNA fragments that contain a particular sequence have...
    712 Words | 2 Pages
  • Recombinant DNA in E. coli
    Recombinant DNA in E. coli Expresses Devastator Enzymes to Clean Oil Spills Dr. Land University of the Pacific, Stockton Abstract The bacteria Sulfolobus Oileatacus has an enzyme called the Devastator, DevA, that helps metabolize crude oil. Since the bacterium is a thermophile, it won’t survive at normal temperatures and cannot be used to clean up oil spills. The devA gene was cloned through the polymerase chain reaction and was confirmed to be present using a restriction enzyme...
    2,774 Words | 8 Pages
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