Growth medium Essays & Research Papers

Best Growth medium Essays

  • Bacteria Growth and Glucose Percentages
    Bacteria’s Growth Affected by Various Glucose Percentages This lab tested whether or not different glucose levels activated bacteria growth. My lab group and I wanted to figure out, if more glucose was spread on the bacteria plates, would there be more bacteria growth in the agar plate. My group predicted that with 25% glucose (the highest percentage of glucose), the bacteria growth would be the greatest. In order to conduct this experiment, my group had 3 ager plates. One plate had 0%...
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  • A Lab Report on Microbial Growth
    David Kennedy Bio 210 Lab Report 1 10/11/13 Microbial Growth Background Information: This lab was conducted in order to understand basic differences among differential and selective media, while recognizing how each media is used to isolate and identify microorganisms (Wistreich, 2003). The first microorganism analyzed was Staphylococcus epidermidis. This organism is gram-positive, single celled, arranged in grape-like clusters, and cocci in shape (Bukhari, 2004). S. epidermidis is...
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  • Bacterial Growth Patterns - 2046 Words
    Bacterial growth patterns and the effects of environmental factors on properties of colonies ERIC LI Abstract In this experiment we work with different types of bacteria and experiment with their different properties and how they grow in certain situations. Different samples of bacteria cultures were gathered from different places such as the mouth, shaded mulch, and from the table top. The samples were collected by using a cotton swab and swiped onto a petri dish filled with nutrient agar. The...
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  • coconut-water mung bean microbial culture medium
    THESIS ABSTRACT 1. Title: COCONUT WATER-MUNG BEAN (CWMB): A MEDIUM FOR MICROBIAL GROWTH a. Total No. of Pages: 201 b. Text No. of Pages: 148 2. Author: TUCAY, CIPRIANO JR., NEAD 3. Type of Document: Thesis 4. Type of Publication: Unpublished 5. Accrediting Institution: University of the Cordilleras Gov. Pack Road, Baguio City CHED-CAR 6. Key Words: Culture medium, bacteria , fungi, Escherichia coli , Staphylococcus aureus,...
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  • All Growth medium Essays

  • Effect of Spices and Herbs on Inhibiting Bacteria Growth
    Research Plan Research Question: What is the antimicrobial effect of common household spices/herbs such as cinnamon, cloves, mustard, ginger, and garlic, on the zone of inhibition in the Staphylococcus epidermis covered nutrient agar? Hypothesis: If 3 teaspoons of chopped cinnamon, cloves, mustard, ginger, and garlic steep in half a cup of boiling water for 15 hours and then placed in nutrient agar covered with Staphylococcus epidermis (gram positive) bacteria for 24 hours (in an incubator),...
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  • Growth media lab report 3 2
     MCB 2010C Lab Report: Growth Media 1. State the difference between chemically defined and complex media in your own words. (2) Chemically define is a media that is pure and contains specific compounds from either organic or inorganic. Also, chemically define media is a chemical composition that is known. On the other hand, Complex media is a complex media that is unknown and is usually not a chemically pure. 2. Classify the media used (YEB, NB, GSB and ISB) as either complex...
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  • Microbiology Notes - 2595 Words
    Review for Microbiology Test #1 (Lesson 1-5) Lesson 1 What is the #1 killer of man worldwide? Heart Disease. What is the #3 killer in the US? Infectious Disease. What is the importance of MO in our world? List 6-8 reasons MO are important. Can’t live in Germ Free World, Keeps Economy running, Agriculture, Medication, Baking, Cosmetics, Paints, Fertilizers, Helps develop immune system, Decomposition of dead plants and animals to enrich the soil. What are the 4 groups of people most prone...
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  • Difco Manual - 11275 Words
    Difco & BBL Manual Manual of Microbiological Culture Media Second Edition Editors Mary Jo Zimbro, B.S., MT (ASCP) David A. Power, Ph.D. Sharon M. Miller, B.S., MT (ASCP) George E. Wilson, MBA, B.S., MT (ASCP) Julie A. Johnson, B.A. BD Diagnostics – Diagnostic Systems 7 Loveton Circle Sparks, MD 21152 Copyright 2009 Becton, Dickinson and Company 7 Loveton Circle P.O. Box 999 Sparks, Maryland 21152 ISBN 0-9727207-1-5 All rights reserved Printed in the United States of America...
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  • Microbiology 150 Lab 3-Selective vs. Differential Media
    Online BIO 150 Introductory Microbiology #3 Lab Report NAME __ Lab Group 2_____ Answer the following questions as you work your way through the lab material typing in your answers. Then submit your finished lab report as a Microsoft Word document. This lab report is worth 100 points towards your final lab grade. Each Q is worth 2 points unless otherwise noted. Also, per the Honor Code, this work must be your own. This is due Mon. 10/8 at 11:59 PM. The theme of this lab is the...
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  • Unknowns Lab Report - 2221 Words
     Unknown #1 Vomit Serratia marcescens & Bacillus cereus Biol 251 Microbiology 5/14/2009 Introduction The purpose of this study is to differentiate and identify two unknown organisms provided by the instructor in a nutrient broth. It is only known that the two organisms are from vomit; one is gram-positive and the other is gram-negative. It is necessary to first separate the two organisms by inoculating a nutrient agar plate...
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  • Lab experiment 7 questions and conclusion
    Experiment 7 Questions 1. Coccus shape bacteria are more structurally sound. 2. Alpha will turn green on the plate because the bacterium is outcompeting the body for oxygen because there is an iron binding site. Beta hemolysis is cellular death which means that when it is present in the body it is releasing an acidic byproduct or a basic byproduct which will crush the structure of the blood cells. Alpha outcompetes with the blood for oxygen while beta hemolysis kills the competition for...
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  • Systematic Identification of Bacillus Subtilis and Serratia Marcescens Through a Battery of Tests and Plates Introduction
    Systematic Identification of Bacillus subtilis and Serratia marcescens Through a Battery of Tests and Plates Introduction: The purpose of this experiment was to use a systematic battery of tube tests and plates designed to lead to identification of two unknown bacterial species, from the combination of all results. A sample of bacteria was used, labeled “Sample 4”, from which both species was to be obtained, one gram positive and one gram negative. Table 1 is a list of the possible bacteria...
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  • Micro Lab Project - 747 Words
     Quantitative & Semi-quantitative Viable Plate Counts Molly Wright, Jenny Cano, Rosa Ramirez BIOL 2420 Lab M/W 4/20/15 Professor Rotibi ABSTRACT: Accurate evaluation of bacterial colonization as a predictive index for alfalfa sprouts has relied on a quantitative culture technique that provides exact colony counts per gram of tissue by culture of five serial dilutions of the alfalfa water. In this study 1 package of alfalfa sprouts were cultured by a semi-quantitative technique...
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  • Unknown Lab Report - 1380 Words
    Introduction The purpose of this lab was to identify unknown bacteria cultures using various differential tests, and my unknown bacteria is #17. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures. The...
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  • ecoli experiment - 1331 Words
    Introduction In my report I will discuss what I did as an experiment and what I hope for it to achieve. Firstly I carried out an experiment to assay the effectiveness that a range of disinfectants have on the growth of ecoli and whether or not it can prevent it from growing. From the experiment i should be able to see that some disinfectants have a greater effect than others do. From this I shall then draw a conclusion and evaluation on what was the most effective, and could there have...
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  • Agar Plate - 5586 Words
    RESEARCH (Rewrite sa Yellow Pad) 1. Different Culture Media in MCR-PRS Laboratory: a. Can be classified according to: a.i. Consistency: a.i.1. Solid Media (contains 2% agar): for colony morphology, pigmentation and hemolysis. a.i.1.a. Nutrient Agar a.i.1.b. Blood Agar a.i.2. Liquid Media (contains no agar): for inoculum preparation, blood culture, and for the isolation of pathogens from a mixture. a.i.2.a. Nutrient Broth a.i.3. Semi-solid Medium (contains 0.5% agar): a.i.3.a....
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  • Hand Washing Lab Outline
    Hand Washing Experiment Outline I. Introduction a. This lab was conducted in order to understand and apply the scientific method. The experiment tested the effectiveness of reducing microorganism (bacteria) with the use of various hand soaps. b. Hypothesis: If the Harris Teeter Foaming Hand Soap is more effective in reducing bacterial growth than the Soft Soap hand soap, then there will be less bacterial/microorganism growth present on the agar plate for the Harris Teeter...
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  • Microbiology Unknown Lab Report
    Lab12-Medical Microbiology- Part1– Differential Media/Biochemical Tests, Sp2012 (Set all of your margins to ½”) Purpose: The purpose of this lab is to help you become a little familiar with some of the tests that can be typically performed in a clinical or research lab facility. These tests may help in determining a particular pathogen’s growth needs. There are several sections to this lab. Find each section and complete the “Preparing for Class” sections. Preparing for class - Day 1 Read...
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  • Culture Media - 1013 Words
    Objective: 1. To familiarize with the method to create culture media. 2. To create solid and liquid media culture. Introduction: Culture medium is a liquid or gel designed to support the growth of microorganisms or cells, or small plants like the moss Physcomitrella patens. There are different types of media for growing different types of cells. There are two major types of growth media: those used for cell culture, which use specific cell types derived from plants or animals, and...
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  • Normal Flora - 584 Words
    Normal flora are found in specific areas of the body and often depends on environmental factors such as pH, oxygen concentration, amount of moisture present, etc. Some sites in which native microbial is the skin in which you often find staphylococci, streptococci, diptheriod bacilli, yeast and fungi. You often find staphylococci, streptococci, diotheriod, spirochetes, and members of the genera Branhamella, Neisseria, and Haemophilus in the throat or the upper respiratory tract. In this exercise,...
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  • lap report - 386 Words
    Prof. Kimberly Rutledge BIO 150 - Unknown Bacterium Identification March 26, 2014 Lap Report Unknown Number : #12 Name of Unknown : Pseudomona aerugninosa The identification of unknown bacteria is a time-honored part of microbiology courses. It will challenge my knowledge and skill in performing laboratory techniques and my ability to critically evaluate the information obtained from these techniques. The unknown bacterium tube number 12 was picked. According to the procedures that we...
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  • identifying gram positive cocci
    IDENTIFYING GRAM POSITIVE COCCI As mentioned in Exercise 8, “Identifying Gram Negative Rods”, identifying bacteria is a common activity in the microbiology lab. Like the game Clue™, each time you gather a piece of information to solve the mystery, you gather some information that supports some identities and eliminates others from contention. In the lab, the process continues as you gather more information until only one microbe remains and all others have been eliminated as...
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  • Lab 4 Introduction To Culturing And As
    Experiment 1- Agar Plate Preparation and Bacterial Inoculation Table 1: Experiment 1 Colony Growth Plate Condition Growth (color, amount, shape, etc.) 1 Surface 1 Many, Large Colonies, Opaque, Round, Irregular, white, yellow 2 Surface 2 Very Few, Small Colonies, Opaque, Round 3 Surface 3 Very Few, Small Colonies, Opaque, Round 4 Control None 5 Airborne Contamination None 1. Which plate grew the most bacterial species (based on the number of different colonies)? Was this a surprise? Why or...
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  • Unknown Lab Report - 1022 Words
    Unknown Lab Report Microbiology Unknown A Sonia Kabra November 26, 2014 Introduction There are numerous reasons for identifying unknown bacteria. Some of these organisms have distinct qualities that set them apart from one another, such as the exposure to certain environments. Through out the semester in the laboratory, we are able to encounter some of the few microorganisms that we as humans have come into contact with. With the knowledge gained from the sessions in the laboratory, we can now...
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  • Micro Lab 1 - 371 Words
    Kristen Magliola 9/9/13 BIO: 414L Lab Report: SOC Medium I. Purpose: To make liquid and solid media for microbial growth II. Background: There are four different types of media used to grow microbes. They consist of Enriched media, Nutrient media, Selective media, and differential media. Enriched media is a growth media supplemented with complex biological molecules. This type of media is used for blood, coagulated blood, amino acids, etc. Nutrient media provides nutrients...
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  • Selective and Differential Media - 616 Words
    Lab Session 8: Selective and Differential Media, Unknown ID (Enzyme Based Tests) Oct. 25-31st (To be turned in PRIOR to start of recitation for lab 8) Name: _____ ________ Objective: Analyze microbes from last week. Understand the use of antibiotics on microorganisms. Gain more knowledge about selective media and differential media. Practice use of the catalase test, coagulase and the oxidase test. Observe microbial flora of the nose. Significance: Understand the use of...
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  • Bacteria - 644 Words
    Lab 11 Methodology By using aseptic, a little cultured bacteria was inoculated on the TSA agar. A quadric streak was making. Inoculation loop was heated and keep it cold for a while before the next quadratic streak. Six agar plates were observed for 24 hour at temperature of 30ºC. Choose one from the dense colony and make a sub-culture on the new agar plate. The step was repeated to get a single colony, which is pure colony. a) Sequestration of bacteria from fish organs Methodology...
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  • Isolation of Individual Colonies - 1087 Words
    A. Define the following: Enriched Media: An enrichment medium contains some important growth factor (vitamin, amino acid, blood component, or carbon source) necessary for the growth of fastidious organisms. Selective Media: Selective media allow for the selection of particular microorganisms that may be present in a mixed culture. Selective media usually contain a component that enhances the growth of the desired organism or inhibits the growth of competing organisms....
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  • Something About Something - 389 Words
    Microbiological Media Table of results: Media | E. coli | Salmonella | Serratia | Pseudomonas | Proteus | EMB(Colour: Wine) | Metallic Green sheen on streak.Media: Pink | ColourlessMedia: Wine | Black dot in centre of colonies.Media: Wine | Similar colour to the media.Media: Wine | ColourlessMedia: Wine | XLD(Colour: Red) | ColourlessMedia: Yellow | Black dot in centre of colonies. Media: Pink | Pink/OrangeMedia: Pink | ColourlessMedia: Pink | Black dot in centre of colonies. (smaller...
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  • The One - 2752 Words
    Fayetteville State University Bio 430 Dr. Allen Spray it or wipe it!!! The scientific study on the effectiveness of CiDecon® Plus Wipes vs. CIDECON ® Q Aerosol on a mixed culture of bacteria (Serratia marcescens Staphylococcus epidermidis) living on publicly used door knobs Fateema Thomas Andrea Donaldson Aryan Jaimangal Tiffany McNeil John Posey Abstract Germs are a mist in every corner of the world. They are stealth and literally, are on everything. This can range from...
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  • Example of a Research Plan - 971 Words
    Research Plan Title: In vitro Antibacterial Activity of Santol (Sandoricum koet jape) at Different pH of Agar School : Ramon Magsaysay (Cubao) High School School Address: Ermin Garcia St., Cubao Quezon City Research Adviser: Mr. Ryan D. Balandra Statement of the Problem The aim of the study is to identify the effect of different pH level of the Agar plate to the antibacterial activity of Santol (Sandoricum koet jape). Specifically, the study will seek for the answer of the question:...
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  • Microorganisms Are Everywhere - 992 Words
    Title: Microorganisms are everywhere. Tara Brady Dt201 Objectives: In this experiment, my aim is to prove that microorganisms are present everywhere and that nowhere in our everyday lives is free from bacteria. I expect this to be the case as even germicide soaps do not kill all bacteria present on our skin; likewise household cleaning products are incapable of killing all microorganisms present. I am doing his experiment to verify the concept that microorganisms are everywhere and I most...
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  • Lab Report - 794 Words
    LAB Report #3 Introduction: In this lab we have focus on Isolation of bacteria from environment. Microorganisms are found throughout the environment: in the air and water; on the surface of any object such as clothes, walls, furniture; in soil and dust; and on and in our own bodies (skin and mucous membranes). In order to demonstrate the ubiquity and diversity of microbes in the environment, samples from immediate areas of the environment and/or from your body will be obtained and...
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  • Lab 1 Ubiquity of Microbes 1
    Lab 1 – Ubiquity of Microbes Bacteria are the most widely distributed organisms in the biosphere. They are found in all different types of soil, water and even in the bodies of other living organisms. Every single place that humans have explored on the Earth, have shown to contain microbes. Bacteria are living organisms and like all living organisms, they require energy and carbon sources in order to metabolize and reproduce. And like all living organisms they respond to changes in their...
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  • Plant Tissue Culture Techniques
    Plant Tissue Culture 151 Chapter 9 Plant Tissue Culture Techniques Lorraine Mineo Department of Biology Lafayette College Easton, Pennsylvania 18042 Lorraine Buzas Mineo (B.S., Muhlenberg College; M.A., Duke University) is a lecturer in the Department of Biology, Lafayette College, and has taught botany since 1978 and supervised the General Biology Laboratories since 1970. Research interests in physiological and forest ecology have culminated in several publications. Other interests...
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  • Abstract: Diffusion Rate of Methylene Blue on Agar Plates at Different Temperatures
    Title: Diffusion Rate of Methylene Blue on Agar Plates at Different Temperatures Abstract: Diffusion is the movement of particles from an area of high concentration to an area of low concentration wherein substances are independent, rapid and in random motion which frequently collide with each other in the sides of the container. This study aimed to assess the influence of temperature on the diffusion rate of methylene blue on agar plates. To determine the relationship between the two...
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  • Phytophthora Isolation - 3049 Words
    Practical guide to detection and identification of Phytophthora Leaf blight A number of Phytophthora species cause leaf blight. These include: P. infestans on potato and tomato; P. palmivora on a large number of tropical fruit species including rubber, durian and macadamia; and P. colocasiae on taro. These blights on leaves are first seen as small flecks but within 3-5 days they expand to produce large lesions. Initially, infected tissue is water soaked but becomes necrotic (brown or black)...
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  • Pglo Transformation - 1825 Words
    Connor Lauffenburger 3/17/13 pGlo Transformation Lab Report I Introduction The purpose of this experiment was to show the genetic transformation of E. coli bacteria with a plasmid that codes for Green Fluorescent Protein (GFP) and contains a gene regulatory system that confers ampicillin resistance. A plasmid is a genetic structure in a cell that can replicate independently of chromosomes. In this lab, the Green Fluorescent Protein, which is typically found in the bioluminescent jellyfish...
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  • Agar plates - 1285 Words
    DIFFERENT TYPES OF AGAR Mac Conkey‘s Agar plate MacConkey (also McConkey) agar is a culture medium designed to grow Gram-negative bacteria and stain them for lactose fermentation. It contains bile salts (to inhibit most Grampositive bacteria, except Enterococcus and some species of Staphylococcus), crystal violet dye (which also inhibits certain Gram-positive bacteria), neutral red dye (which stains microbes fermenting lactose), lactose and peptone. QUALITY CONTROL Results after 24 hrs...
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  • The Role of Agarase in Agar-Degrading Bacteria
    The Role of Agarase in Agar-Degrading Bacteria Abstract Agar-Degrading (agarolytic) Bacteria is physiological class of bacteria capable of utilising agar as a sole carbon source. This ability is made available by the use of agarases - enzymes which break down agarose into oligosaccharides. This physiological class branches through genii, regardless of Gram Stain status or morphology. Through a review of scientific literature we can find identification methods, optimum conditions and...
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  • Microbiology Coursework: Bacillus Cereus
    Microbiology Coursework: Bacillus cereus After investigation following on outbreak of food poisoning at a pizza restaurant, it was found that all suffers had consumed a portion of side salad from the self-service salad bar alongside their main dish. Subsequently, this was further traced to a rice salad. Environmental Health Officers investigating this outbreak suspected it may have been caused by Bacillus cereus (B. cereus). The presence of large numbers of B. cereus in a food is...
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  • Microbiology Module 02 Homework Assignment
    Microbiology Module 02 Homework Assignment Use the information presented in this module along with additional outside research to answer the questions: 1) Compare and contrast prokaryotic and eukaryotic. a) Prokaryotes and eukaryotes are two types of cells that are very different but share some certain properties such as methods of reproduction, protein synthesis, an organized metabolism, response to stimuli, and plasma membranes. One significant difference is that prokaryotes are without a...
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  • Microbiology: Viable Counts - 828 Words
    INTRODUCTION Total Viable Count is a quantitative idea about the presence of microorganisms such as bacteria, yeast and mold in a sample. It counts the number of colonies produced by a very dilute suspension of bacteria on an agar plate and to observe the differential staining behaviour of the living bacteria. This involves counting the colonies produced by viable cells under favourable growth conditions. Some techniques needed before the viable count, like pour plate method, spread plate...
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  • MLT Task 3 - 296 Words
    MLT1 Task 3. Isolation of Individual Colonies A. Enriched, complex and synthetic media are microbiological media. Enriched media is a medium containing growth factors. Complex media is composed of unknown quantities of proteins and extracts. Synthetic media is composed of a known quantity of chemicals used. They provide different environments for bacterial growth. 1. Enriched media can be used to grow fastidious organisms, which will benefit greatly from growth factors. Complex media can...
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  • Pure Culture Lab - 409 Words
    Jody Kaur Pure Cultures Lab 1/31/13 Introduction : Pure cultures are made of only one type of organisms and can be used to study their properties. A method used to isolate pure cultures is making a steak-plate, which is a dilution process in which culture is spread over an agar plate in a certain manner. Using a loop rod, culture was taken from the tube and dragged across area 1 several time,of the agar. The agar was then turned 90º, and the loop was flamed and cooled. Taking some...
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  • Lab 1 introduction - 8692 Words
    BIOL3150: Microbiology Summer 2014 Laboratory Manual Calendar description: Fundamentals of microbiology; microbial organisms; microbe-host interactions; microbial genetics and evolution; microorganisms and human disease; environmental and applied microbiology. Three lecture hours, three lab hours. One term. Four credits. Prerequisites: SC/BIOL 2020 4.00; SC/BIOL 2021 4.00; SC/BIOL 2040 4.00. Course Credit Exclusion: SC/BIOL 3150 3.00. Course director: Dr. Michael Gadsden...
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  • Microbiology Unknown - 429 Words
    Microbiology Unknown 5/7/2013 Microbiology Unknown #94 The purpose of this report was to identify an unknown bacterium by applying methods that were previously learned and conducted in microbiology lab class. It is possible to identify a mixed culture by running experiments on the unknown bacteria and taking what we already know and applying it to the results. It helps to slowly eliminate any bacteria that do not correspond with the results of certain tests. An unknown test tube labeled 94...
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  • Final Unknown Lab Report Burel
    Joanna Burel 04/20/15 Dr. Leonard Prassanna Rao Microbiology BIO 2010 Section 03 Isolation and identification of unknown bacteria ABSTRACT A mixture of two unknown bacteria was obtained and handed out in microbiology lab. It contained one gram positive and one gram negative bacteria. The gram positive rod bacteria had small round yellowish morphology able to grow at 37 degrees Celsius. It was found to be a facultative anaerobe, oxidase positive, fermenting glucose and MR, VP,...
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  • Intro to Micro Review Questions
    Intro to Micro Review Questions Review questions are designed to help you study for exams. They are not intended to cover every question that may be on the exam. Questions on the exams may be over any of the material covered in class lectures and in your reading assignments. Review questions are arranged by topic, not by exam. Refer to the Lecture Schedule and your class notes for topics that will be covered on a specific exam. Introduction to Microbiology 1. What is microbiology? What...
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  • Zone of inhibition - 833 Words
    Introduction: Antimicrobial agents are chemicals that are used against bacteria. There are many such agents available. Because there are many different situations where bacterial control is important, no antimicrobial agent is effective in all situations. For example, you wouldn't use the same compound to fight an ear infection as you would use to sterilize surfaces in an Operating room. The situations are completely different. In one case, you are trying to assist the body to fight off an...
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  • Unknown Lab Write Up - 1918 Words
    Test Rationale Observation Conclusion Gram Stain When a group of cells is stained with Crystal Violet and with Iodine, Ethyl Alcohol is used to remove any color it can. A counter stain is done to help identify one type with Safranin. If the cell type is gram positive it will remain purple because the Ethyl Alcohol could not remove all of the Crystal Violet. If it is Gram- then the violet color would be gone and only a red counter stain would remain. G+=Purple Color G-=Red Color Gram -...
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  • BIOS242 W3 Lab Template
    Name Lab, Week # 3 Experiment : BACTERIAL GROWTH AND CONTROLLING BACTERIAL GROWTH Introduction Procedure < Include information that the reader would need to repeat your experimental procedure. Do not include any observations or results in this section> Observations and Results Part I: Bacterial Growth Result + or - Appearance after Incubation Gram Stain N/A OF Glucose...
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  • Antibiotic lab report - 881 Words
    ANTIBIOTIC RESISTANT BACTERIA LAB REPORT Introduction: Only 1% of bacteria are harmful for humans however there are methods of preventing the spread of the harmful bacteria. Chemical substances that are used to either kill or inhibit growth of bacteria are known as antimicrobial agents, of which the three basic types are disinfectants, antiseptics and antibiotics. Disinfectants are chemicals used to inhibit growth or kill bacteria on living tissues, antiseptics inhibit growth or kill...
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  • Miss - 380 Words
    Survey of Nasal Carriage of Staphylococcus aureus in Microbiology 1 Students at RMIT Aim: To determine the carriage rate of Staphylococcus aureus in the nares of students taking second year microbiology courses at RMIT. Introduction: Carriage of S.aureus is important in hospital patients, preoperative patients, hospital staff, food handlers etc. because it carriage of S.aureus appears to play a key role in the epidemiology and pathogenesis of infection. S.aureus can cause localized and...
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  • Freeze Exim - 7992 Words
    CHAPTER-1 INTRODUCTION 1 INTRODUCTION The organisation can be defined as “the planned coordination of the activities of a number of people for the achievement of some common, explicit purpose or goal, through division of labour and function and through a hierarchy of authority and responsibility.” Organisations are not just means used by groups of people to achieve some goals. They present different images like,       Organisations as machines Organisations as living systems...
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  • Isolation and Purification of Lyngbya majuscula on Nutrient-enriched Agar Plates
    Isolation and Purification of Lyngbya majuscula on Nutrient-enriched Agar Plates A Special Problem Isolation and Purification of Lyngbya majuscula on Nutrient-enriched Agar Plates ABSTRACT Lyngbya majuscula samples were obtained from the Phycology Laboratory stock culture of the UPV Institute of Aquaculture. Five (5) mm fragments of these were inoculated into agar plates that used 1% agar concentration enriched with varying concentrations (1.0%, 1.5%, and 2.0%) of Hughes,...
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  • Biology Lab - 502 Words
    Lab 5: culture transfer instruments, techniques and isolation and maintenance of pure cultures. Abstract Background: Pure cultures of multicellular organisms are often more easily isolated by simply picking out a single individual to initiate a culture. This is a useful technique for pure culture of fungi, multicellular algae. To aseptically transfer sufficient bacteria to an inoculated agar slant to start a new pure culture Aim: the aim of this experiment is to correctly use the pipette,...
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  • 48506933 AS Edexcel Biology Core Practical Summary
    CORE BIOLOGY PRACTICALS You will need to know these practicals as the exam board may ask you questions based on them. Below is a summary of each one. Name of practical and independent & dependent variables Effect of caffeine on Daphnia heart rate Independent: caffeine concentration Dependent: heart rate of Daphnia Measuring the content of Vitamin C in fruit juice Independent: fruit juice Dependent: volume of juice required to decolourise 1cm3 of DCPIP The effect of temperature on cell...
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  • The Investigation of Microbial Diversity in Lichen and Hairy Cap Moss on Mt. Arabia
    The Investigation of Microbial Diversity in Lichen and Hairy Cap Moss on Mt. Arabia By: Prajoth Pattamatta Biology 141 Wednesday 2:30-5:30pm Dr. Steve Baker November 22, 2011 Abstract The research experiment observed and analyzed the differences in microbial diversity between lichen on bare rocks and hairy cap mosses in the annual community. Samples from three different sites were taken from Mount Arabia in...
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  • Biochemical Action of Bacteria - 3616 Words
    OBJECTIVE: 1. To distinguish the bacteria abilities to metabolize various substrates and end products formed. 2. To observe the growth of different bacteria species in term of structures and its morphology based on different chemical substance applied. 3. To observe physiological and immunological properties utilized by different species of bacteria. INTRODUCTION: Bacteria biochemical testing can determine the types and numbers in terms of colony forming units of bacteria...
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  • Escherichia Coli and Staphylococcus Albus
    Escherichia coli and Staphylococcus albus Some bacteria's require a certain amount of light in order to function properly and there fore survive. Escherichia coli is found in the gut and intestines of mammals. Staphylococcus albus is generally a skin dwelling bacteria Aim: To test whether the amount and type of light that the bacteria's E.coli and S.albus are subject to causes changes in their growth rates and habits. To test whether the growth rates and habits of the bacteria's E....
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  • Shake Flask Fermentation - 3192 Words
    Table of Contents 1.0 Abstract 2 2.0 Introduction 2 3.0 Aims 3 4.0 Theory 3 5.0 Apparatus 5 6.0 Procedure 5 7.0 Result 7 8.0 Calculation 10 9.0 Discussion 11 10.0 Conclusion 13 11.0 Recommendation 13 12.0 References 13 13.0 Appendix 14 1.0 Abstract In this experiment, Escherichia coli is used as a sample to study the growth kinetic of microorganism in shake flask. A different volume of E.coli was transferred into 250ml Erlenmeyer/shake flask containing media for the...
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  • E3 Lab Procedure - 5259 Words
    Biol 2281, Summer 2013 E3: Microbial Techniques Procedure/Report Handout Experiment 3: Microbial Techniques Objectives: By the end of this lab, you will be able to: 1. Understand and practice aseptic techniques in handling microorganisms. 2. Learn simple media preparation procedures 3. Streak plates and spread plates to obtain single colonies. 4. Understand how serial dilutions are made and how cell density can be determined through standard plate count. 5. Learn to use micropipettes and...
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  • Unknown Project - 2236 Words
    Traditional Analysis of Unknown Colony Morphology Color- light yellow pigmentation Form- circular Elevation- convex Margin- entire [staphylococci] DNase Test This test is used to detect if the bacteria contains any deoxyribonuclease activity. Because no color change was observed from blue to clear my unknown bacteria displayed a negative result. Blood agar plate This test is used to detect the hemolytic activity in the bacteria. A darkish green color on the media around...
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  • Lab: Sampling Bacteria - 809 Words
    Lab: Sampling Bacteria Purpose: Refer to handout sheet. Materials: Refer to handout sheet. Procedure: Refer to handout sheet. Pre-Lab Questions: 1. Why is one dish being reserved for the class as a "control"? Having a controlled variable is important in order to be able to look at what the bacteria would look like if it hadn't been contaminated and just left as agar. Having a sample of agar that wasnt exposed to any bacteria will provide a clear picutre of what grew on the...
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  • DONETask 2 - 661 Words
    A. Describe the difference between an obligate and facultative aerobe. An obligate aerobe is a microorganism that must have oxygen for growth and will grow at the top of the test tube. A facultative aerobe prefers oxygen but it is not necessary for growth. Many of these microorganisms will grow at the top of the tube, but will also be dispersed throughout as well. B. Discuss the temperature requirements of the organisms (you grew) in this lab. Both Staphylococcus epidermis and Lactobacillus...
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  • Vegetarianism and Agar - 2585 Words
    Agar From Wikipedia, the free encyclopedia Jump to: navigation, search Not to be confused with auger or augur. For other uses, see Agar (disambiguation). Culinary usage Mizuyōkan - a popular Japanese red bean jelly made from agar. Scientific usage A blood agar plate used to culture bacteria and diagnose infection. Agar or agar-agar is a gelatinous substance derived by boiling[1] a polysaccharide in red algae, where it accumulates in the cell walls of agarophyte and serves as the primary...
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  • staphylococcus aureus - 858 Words
    Abstract This paper explores the study of an unknown bacterial culture. The bacterium were randomly assigned in a double blinded fashion to alleviate scientific or experimental error in determining their gram stain, morphology, arrangement, and scientific classification. There were eight unknown bacterial cultures given, with the unknown bacterial culture being one of the eight bacterial cultures. A myriad of tests and experiments were performed on the unknown bacteria in order to be able...
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  • Double Unkown Final Project
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  • Microbiology Labs - 6972 Words
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  • Biology ia - 1370 Words
    002141-0012 Nuba Jackson IB Biology Microbiology IA How effective is Lysol in the reduction of bacterial growth compared to Pinesol in reduction of E. Coli growth in agar at room temperature?  Background Information: Pinesol and Lysol are both common household disinfectants that make very big commercial claims; both claim to kill 99.9 percent of bacteria. Lysol...
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  • microbiology final exam unknown bacteria report example
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  • Unknown Bacteria - 445 Words
    Identifying Unknown Bacteria Using Biochemical and Molecular Methods Beginning of Instructor Pages Instructor Pages - - 3 Purpose The purpose of this lab is to introduce a variety of lab techniques to students working on the common problem of identifying an unknown bacterium. This lab helps students develop an understanding of the biochemical and molecular differences in bacteria and introduces the concept of identifying species based on characeristic gene sequences. Students work...
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  • disinfectant and antibiotics effect on bacteria method
     Method 1. Sterilise all of your equipment with ethanol, blue flame or disinfectant if you can flame it. 2. Collect all equipment together ensuring you collect your goggles and gloves as you are using a flame and gloves and goggles are part of safety precautions. (don’t wear gloves with messing with Bunsen burner only goggles, but use gloves when messing with chemicals ensuring goggles on all the time) 3. Clean your surface with disinfectant to sterilise your lab work surface, this will...
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  • Banana Tissue Culture - 1832 Words
    BANANA TISSUE CULTURE INTRODUCTION Plant Tissue Culture is culturing of any part of the plant in a specially defined growth media under aseptic laboratory condition in Petri dishes, test tubes or in any other suitable glass containers. The plant nutrient media consists of macro and micro salts, vitamins and desired levels of plant growth hormones. Depending upon the plant species genetic nature and with help of above supportive media various forms of callus/embryos/shoots/ roots or direct...
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  • Aseptic Technique and Transfer of Microorganisms
    Introduction: Microbes, also called microorganisms, are minutes living things that individually are usually too small to be seen with the unaided eye. The group includes bacteria, fungi (yeast and molds), protozoa and microscopic algae. It also includes viruses, those noncellular entities sometimes regarded as straddling the border between life and nonlife. People tend to related these microbes only with major disease such as AIDS, uncomfortable infections, or such common inconveniences as...
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  • Microbiology Lab Report - 767 Words
    Introduction ! Identifying an unknown bacteria can be an arduous journey. The journey starts out by performing a gram stain and then performing various tests. The first part should not be challenging. The challenging part is analyzing your results and using the data to infer what your unknown organism might be. ! When I was first handed my organism, I performed a rather unconventional test: I smelled it. Throughout the semester of dealing with various organisms, I had learned that...
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  • Culture Media - 586 Words
     Use of differential, selective and enriched media: EMB, blood and starch agar. OBJECTIVES: distinguish between different bacterial species based on colony morphology on agar plates To distinguish the growth characteristics of microorganisms in various differential, and selective media. Differentiate bacteria based on their ability to hydrolyze starch. Materials: Plates of EMB, Starch and blood agar. Stool sample. Inoculating loop. Bunsen burner. Soil sample. Cotton soap....
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