Chromatography Essays & Research Papers

Best Chromatography Essays

  • Chromatography - 275 Words
    Chasyn Carter 1/8/13 Biology Chromatography Chromatography is the term for a set of lab techniques for the separation of mixtures. The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase. The Russian-Italian biochemist Mikhail Semyonovich coined the term "chromatography" and published the first paper on the method in 1903. Chromatography has many uses in biology. It’s used to separate and identify...
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  • Chromatography - 615 Words
    Chromatography: The colours separate and move up the paper at different rates Chromatography can be used to separate mixtures of coloured compounds. Mixtures that are suitable for separation by chromatography include inks, dyes and colouring agents in food. Simple chromatography is carried out on paper. A spot of the mixture is placed near the bottom of a piece of chromatography paper and the paper is then placed upright in a suitable solvent, eg water. As the solvent soaks up the paper, it...
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  • Chromatography - 1688 Words
    Chromatography Abstract Paper chromatography is one of the methods under chromatography, it can use in identifying unknown compounds using known compound and it can also use as a separation technique based on the differences in affinities of components of the mixture to a stationary phase and a mobile phase. In the experiment, the stationary phase was the filter paper onto which the dye samples were dropped onto while the mobile phase was the solvent mixture containing ethanol and water...
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  • Chromatography - 993 Words
     Chromatography – Analyzing Analgesics by TLC and Isolation of β-Carotene by Column Chromatography Introduction/Background: Flavonoids are an important group of additives that can be defined as pure substances either natural, extracted from raw materials or synthetic. Chromatography is the separation of two or more compounds or ions caused by their molecular interactions with two phases – one moving and one stationary (Weldegirma 2012). Three types of...
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  • All Chromatography Essays

  • Chromatography - 891 Words
    Chromatography (Greek for ‘colour writing’) is used to describe various methods applied to separate mixtures (referred to as the sample of the experiment) with great accuracy to analyze them. By using chromatography we can manipulate these to move at different speeds through the system, thus separating them. Chromatography is necessary in chemical industries, as well as bio processing companies. Chromatography can be: 1. analytical: used to measure ratios of analytes(substance in simpler...
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  • chromatography - 1280 Words
    Column and Thin layer Chromatography of Red Siling Labuyo Marian Angelu Ramos, Rose Ann Refuela, Leomarie Duanne Sanchez, Paula Lynne Santos, Geraline Sarmiento, Jon Carlo Semana Group number Seven, 2E- Pharmacy, Faculty of Pharmacy, University of Santo Tomas ABSTRACT Chromatography is the separation of mixtures into their constituents. It relies on the differential solubilities or absorptivities of the components to be separated with respect to two phases, one of which is stationary and...
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  • Chromatography - 2000 Words
    COLUMN AND THIN LAYER CHROMATOGRAPHY Maria Janine B. Abarientos, Kuia B. Allarde, Aliana Keshia P. Andino Mary Viadelle E. Andrada and Nina Marian Robelea G. Ang Group 1 2C Pharmacy Organic Chemistry Laboratory ABSTRACT In this experiment, the techniques column and thin layer chromatography was used to separate and determine the purity of the colored components of siling labuyo and malunggay leaves. The results obtained relied on differential solubilities and adsorptivities of the...
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  • Chromatography - 884 Words
    Chromatography: How can we separate a mixture? Purpose The chromatography lab is to understand how molecules with similar molecular properties can be separated with paper chromatography. These differences will be interpreted to see the distinction of separate chemical substances. Pre Lab Questions 1. Explain capillary action as it pertains to water and paper. Capillary action makes water draw up the paper. As paper absorbs water mixes with the solutions in the paper. 2. What is...
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  • Chromatography - 684 Words
    Direction: Read the article below. Answer the questions beside the article by checking the number of the sentence where you can find the answer. Then, write the specific answer on the space provided for. Good luck!!! CHROMATOGRAPHY (By: Mayflor Markusic) KALIKASAN: Everyday Science in Action Volume 4 No. 1; pages 22-24 a) What is chromatography? 1 2 3 4 ____________________________________________________________ ________________ b) Give examples of...
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  • Chromatography - 969 Words
    Paper chromatography is one of the easiest methods of chromatography. It is a method of planar chromatography (stationary phase is in form of a plane). Paper chromatography follows the basic principle of chromatography, which states that substances or components are distributed in between the stationary phase and the mobile phase. It is an analytical technique, where only a small amount of a sample is used for separating and identifying its components. Like any other method of chromatography,...
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  • Experiment on Chromatography - 2527 Words
    ------------------------------------------------- Using Liquid-Liquid Partitioning Chromatography Teshia Faye T. Josue Institute of Chemistry, University of the Philippines, Diliman, Quezon City July 10, 2013 July 16, 2013 I. II. Methodology Chromatography is a method of separation that has been familiar to students from the time they take up Chemistry in their high school years. Specifically though, it was the Paper Chromatography that was introduced. Different colors of inked...
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  • Paper Chromatography - 525 Words
    A solution of the mixture to be separated is "spotted", usually from a micropipet, near one edge of a piece of filter paper, and the solvent is evaporated. Usually several sample and standard spots are placed along the edge. Then the chromatogram is "developed" by immersing that edge of the paper in a solvent that migrates through the paper as the mobile phase. The solvent often has two, three or four components, one of which is usually water. Development is normally done in a chamber that is...
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  • Chromatography Lab - 615 Words
    Chromatography Lab 1. The purpose of this lab was to observe the process of purifying the green fluorescent protein (GFP) in bacteria through hydrophobic interaction chromatography (HIC). Since GFP is very hydrophobic when the supernatant containing GFP is being purified through HIC column that contains a highly salty binding buffer the hydrophobic GFP sticks to the HIC beads. The other hydrophilic proteins pass through the column. The first day of lab we performed lysis of the bacterial cells...
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  • Thin Layer Chromatography and Column Chromatography
    Introduction Chromatography is a technique used to separate a mixture into its individual components. In this experiment, chromatography is done by using a thin layer chromatography (TLC) plate and filter paper. The word ‘chromatography’ itself means the separation of colours. However, nowadays, both coloured and colourless compound are used in chromatography by various methods. In chromatography, the separation process of mixture is mainly based upon the polarity and molecular weight of...
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  • Gas Chromatography - 1426 Words
    Gas Chromatography Purpose: The purpose of the gas chromatography lab is to find out how different substances interact with the surface of a solid. Chromatography is a separation technique that depends on the relative distribution of the components of a mixture between a mobile phase and a solid stationary phase. Chromatography measures the tendency of a substance to interact with the surface of a solid or to remain in a mobile phase. When doing a chromatography lab the mobile phase has...
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  • Paper Chromatography - 1569 Words
    EXPERIMENT 5: CHROMATOGRAPHY Abstract Paper Chromatography uses a chromatogram paper as its stationary phase and the solvent as the mobile phase. Retention factor is the ratio of the distance travelled by the sample to the distance travelled by the solvent. This experiment aims to separate organic compounds, to compute Rf values and to identify unknown compounds using Rf values. The ten samples underwent paper chromatography to determine the components of the unknown sample. The mobile phase...
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  • Paper Chromatography - 588 Words
    Analysis of Food Colorings by Paper Chromatography Introduction Paper Chromatography one method for testing the purity of compounds and identifying substances. This is a useful technique because it is relatively quick and requires small quantities of material. Separations in paper chromatography involve the same principles as those in thin layer chromatography. The substances are distributed between a stationary phase and a mobile phase. The stationary phase is...
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  • 5.05 Chromatography - 678 Words
    Title Color Chromatography Lab Background Information and Research 1. Paper chromatography is a way separate the components of a mixture. 2. It can be used for purification, separation of mixtures, and identify of components. Purpose The purpose of this experiment is to take a look at what chromatography is really about. It helps us see what we don't normally see, such as all the hidden colors that show up in the different colors. Materials Four different markers. I used Purple,...
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  • Chromatography Lab - 4299 Words
    Environmental Health Sciences Center Community Outreach & Education Program CHROMATOGRAPHY (Adapted from: Forensic Science Activities. University of Colorado Boulder Hughes Initiative. .) DESCRIPTION: Students will use paper chromatography to separate ink molecules and identify the pen used on an unknown sample of handwriting. Students will graph and analyze data they collect using paper chromatography. PURPOSE/GOAL: Students will be able to: • Gain understanding of the purpose of...
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  • Candy Chromatography - 305 Words
    Have you ever wondered how many dyes are in your favorite candies? Candy chromatography is a method of separating and comparing dyes used in candy. It is a complex mixture, without having to taste it. The word chromatography is broken into two Greek words, “ chroma” meaning color and ‘graphein’ meaning to write. It works because the components of the mixture will be different in how much they “stick” to each other, and to other substances. The dyes that stick more tightly to the paper fibers...
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  • paper chromatography - 595 Words
    Paper chromatography Presented by – Miss. Shruti Vilas Kharat. SYBSc / FS13164 History- Paper Chromatography (PC) was first introduced by German scientist Christian Friedrich Schonbein (1865). PC is considered to be the simplest and most widely used of the chromatographic techniques because of its applicability to isolation, identification and quantitative determination of organic and inorganic compounds. Definition- Paper chromatography is an analytical method technique for separating...
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  • Paper Chromatography - 615 Words
    Paper Chromatography Chromatography comes from the Greek word khromatos (color) and graphein (to write); i.e. paper chromatography is literally color writing on paper. This method tests the purity of compounds and identifies substances. This analytical process, despite having been replaced by the success of thin layer chromatography, still stands as a valuable teaching tool, and is nevertheless very common. This method is very useful because not only is it a relatively quick process, but...
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  • Gas Chromatography - 707 Words
    GAS CHROMATOGRAPHY EXPERIMENT The purpose of this experiment is for the student: 1) to learn the general theoretical aspects of gas chromatography as a separation method, 2) to learn how to operate gas chromatographs specific to COD, 3) to become familiar with using the gas chromatograph (GC) to qualitatively identify components of mixtures, 4) to be introduced to and to interpret the quantitative data available via gas chromatography, 5) to gain insight into how the GC technique is...
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  • Chromatography Experiment - 823 Words
    In this experiment the objective is to separate dyes in washable markers to see how many different dyes are combined to create one color. The technique used to separate the dyes in this experiment is called chromatography. The materials used consisted of – 3 pieces of chromatography paper, a tall clear glass, water, table salt, 1 tsp. measuring spoon, an 8 oz. measuring cup, pencil, ruler, 6 washable markers, and paper towels. The markers used in this experiment have to be water soluble...
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  • Gas Chromatography - 826 Words
    Neha Bhattacharjee 5/23/11 Upamanyu Basu Gas Chromatography Part 1: General Questions 1. Gas chromatography is a type of chromatography that allows for separation and analysis of a compound. It does this by vaporizing the sample then using a carrier gas to push it through a column which then separates it based on an equilibrium formed between solution and dissolution. 2. As a gaseous mixture travels down the column parts of the sample dissolve...
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  • column chromatography - 638 Words
     Column Chromatography of Plant Pigments Sopheea Celine Pizarras*, Anne Morielle Ponciano*, Emerson Quimba*, and Aimee Reyes* College Of Science, University of Santo Tomas, España blvd., Manila Abstract: Column Chromatography is a solid-liquid adsorption chromatography. It depends on the essential principles so as in thin layer chromatography. This separated and analysed the different components of Capsicum frutescens, siling labuyo with the use of reagents such as Hexane, Dichloromethane,...
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  • Chromatography of Dyes - 492 Words
    ABSTRACT Separation and identification of components of a mixture was determined by using Chromatography. The components of a mixture have different affinities for a stationary phase as well as different affinities for a mobile phase. INTRODUCTION An extremely common technique by using two chemicals and filter paper can give you sufficient information. A well-done Chromatography of dyes will provide you with enough data to determine if a liquid mixture is heterogeneous or homogenous . In a...
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  • Chromatography of Pigments - 665 Words
    Introduction Paper chromatography is a method using a chromatography paper to identify the many mixtures of one simple color. The word chromatography comes from the Greek words, "chroma," meaning color, and "grafein," meaning to write. The process of this experiment is to place a colored dot, such as marker ink, onto the chromatography paper, one centimeter away from the triangular tip. Next dip your chromatography paper into the solvent, not allowing it to touch the colored dot. Let it sit...
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  • Candy Chromatography - 452 Words
    Candy chromatography is a method of analysis of separating and comparing of dyes used in candies and food coloring. It is also a proven method to analyze candy and its complex mixture, without having to taste it. These complex mixtures can be separated back into pure substances. To find out the ingredients of a favorite candy, chromatography would help to define every ingredient found in the candy sample. The word chromatography is derived from the two Greek words, "chroma" meaning...
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  • Chromatography and NMR - 2953 Words
    Binding curves: CD can be used to monitor the binding if s substrate to a protein. The substrate can give a very different CD spectrum when free in the solution relative to when bond in solution. Outside of farUV: 180-240nm. 1. Near UV CD: 240n-320nm, Aromatic amino acids and disulphide bonds. 2. Visible CD: d-d transition in some metal protein complexes for eg Cu (II) prion. Principles of Chromatography...
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  • Gas Chromatography - 513 Words
    group 2Gas Chromotography Mass SpectrumLab ManualNOrth Carolina A&T State University chemistry DepartmentPatrick Mills Erin Barnes | | | | | | | January 24, 2013 Introduction: Chromatography is the separation of components of a mixture by differential adsorption between a stationary phase and a mobile phase.1 GC separates molecules as it moves through the column. There are two different column types that are used in gas chromatography, packed and capillary.3 The...
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  • Candy Chromatography - 605 Words
    Chromatography is a physical method of separation in which the components to be separated are distributed between two phases, one of which is stationary (stationary phase) while the other (the mobile phase) moves in a definite direction. It separates the analyte to be measured from other molecules in the mixture and allows it to be isolated. Chromatography may be preparative or analytical. Preparative chromatography seeks to separate the components of a mixture for further use (and is thus a...
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  • Chromatography Lab - 1239 Words
    Problem How can you tell pigment separation by using Chromatography? Objective To prepare a chromatogram, separate pigments in a leaf and interpret the chromatogram. Hypothesis If I am to put a chromatography paper into a solvent, then it would separate the pigments depending on their Rf value. I think the pigments will separate in this order: Chlorophyll A, Chlorophyll B, Carotene, and Xanthophyll. Introduction Chlorophyll is the molecule that absorbs sunlight and uses its energy...
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  • Column Chromatography - 1689 Words
    Column Chromatography of Plant Pigments Paul Ibarbia, Gene Paolo Jasmin, Gianpaolo Jimenez and Lorenzo Labicane* Department of Biology, University of Santo Tomas, Manila, Philippines Abstract Column chromatography of plant pigments is the separation of plant pigments extracted from Capsicum frutescens (siling labuyo). No slurry is prepared of the eluent; eluent with the stationary phase is set and then carefully poured into the column. The eluent are the solvents which are responsible for...
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  • Column Chromatography - 1301 Words
    Column Chromatography of Plant Pigments Jaybee Balilea, Sharmaine Baysic, Maria Anjelette Patricia Belen 3Bio-7, Department of Biological Sciences, University of Santo Tomas, Manila, Philippines Abstract Column Chromatography is a form of solid-liquid adsorption chromatography and depends on the essential principles as does in thin layer chromatography. It was used in this experiment in separating and analyzing the different components of Capsicum frutescens (siling labuyo) with the use...
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  • Advantages and disadvantages of paper chromatography and thin layer chromatography
    Advantages of thin layer chromatography (TLC) TLC is very simple to use and inexpensive. Undergraduates can be taught this technique and apply its similar principles to other chromatographic techniques. There are little materials needed for TLC (chamber, watch glass, capillary, plate, solvent, pencil, and UV-light). Therefore, once the best solvent is found, it can be applied to other techniques such as High performance liquid chromatography. More than 1 compound can be separated on a TLC...
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  • Tlc and Paper Chromatography - 562 Words
    Thin Layer Chromatography/ Paper Chromatography Lab # 10 11/26 Sample # 32 for TLC Sample # 1 for Paper Introduction: Chromatography is one of the most important separation techniques used in all fields of chemistry ranging from analytical chemists to pharmacists. The understanding of how chromatography works and how to operate instruments used to carry out the procedures is an important lab technique to learn. Experiment: Thin Layer Chromatography. Lab #10-1 Paper...
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  • Thin Layer Chromatography - 535 Words
    It was concluded that the product created was paracetamol when a thin layer chromatography test was performed and observed under ultraviolet light (which is discussed further down the page). An unknown substance was treated with acetic anhydrate and resulted with paracetamol. A compound that behaves in this matter is 4-aminophenol and is widespread in the industrial production of this drug. Upon these observations was the build up of “Chemical A” decided. When 4-aminophenol is t treated...
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  • Chromatography of Commercial Analgesics - 890 Words
    Chromatography of Commercial Analgesics Using the thin layer chromatography the major components of a commercial analgesic tablet will be indentified and purified. The active ingredients of the analgesic used were characterized by comparison with chromatograms of aspirin, acetaminophen, ibuprofen, mefanemic acid, and caffeine through the use of UV chromatography then the Rf value was calculated for each solvent. INTRODUCTION In this experiment, thin-layer chromatography (TLC) will be...
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  • Chromatography Lab Repot - 668 Words
    Design and carry out a scientific investigation on any one factor that affects retention on paper chromatography Design and carry out a scientific investigation on any one factor that affects retention on paper chromatography CHEMISTRY LAB REPORT CHEMISTRY LAB REPORT Name: Sri Danielle Class: 9A Teacher: Sir Ajay Name: Sri Danielle Class: 9A Teacher: Sir Ajay Aim My aim for this experiment is to find out how different types of solvent will affect the retention factor in paper...
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  • Chromatography Analysis of Black Pens
    Chromatography Analysis of Black Pens to Determine Unknown Sample Purpose: Paper chromatography was performed on five different black pens, using four different solutions to determine which would be most appropriate to use on an unknown sample. Paper chromatography was then performed on the unknown sample to identify which pen was used to create it. Procedure: Each person participating in the analysis was assigned one solution to work with: V. Temple used distilled water, D. Sellers...
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  • Isolation of Essential Oils and Chromatography
    Experiment 4 CHROMATOGRAPHY OF PLANT PIGMENTS Separating impure mixtures into pure substances is among the most important and fundamental of skills which must be learned by a chemist. The study of pure substances requires that the chemist learn many different techniques of separation so as to pursue the study of properties of pure substances and their reactions. It was Alexander Tswett, a Russian botanist, who first found that he could extract pigments from plants. This...
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  • CHEM 2204 Chromatography Lab
    CHEM 2204 Chromatography Lab by wyk.wong » Fri Jul 11, 2014 10:25 am Results and Calculations Rf values Rf=(Distance moved by the spot (cm))/(Distance moved by the solvent front (cm)) Toluene: Rf=2 cm/3.8 cm=0.53 (Fluorenone) Rf=1.1 cm/3.8 cm=0.29 (Fluorene) Hexane: Rf=1.8 cm/2.2 cm=0.82 (Fluorene) Rf=0 cm/2.2 cm=0 (Fluorene Table 1: Experimental IR peaks compared to literature IR peaks for fluorenone Functional group Experimental peak (cm-1) Literature peak (cm-1) C-H 3010.5...
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  • Thin Layer Chromatography - 1618 Words
    INTRODUCTION The purpose of this experiment is to identify the components of an unknown analgesic drug substance by comparing the Rf values of its composition compounds with those of reference compounds using thin layer chromatography. The unknown to be identified is unknown #101. This thin layer chromatography experiment is made of two major parts: The first part, samples of the unknown solution and the standard solutions are spotted on a TLC plate that we dip into a developing chamber...
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  • Chromatography Lab Report - 677 Words
    Chromatography lab Purpose: To separate food colorings into their component dyes using paper chromatography. Materials: Chromatography paper, Food coloring, Ruler, Pencil, Solvent solution, Test tubes, Test tube rack. Safety precaution: wear aprons, to make sure that you don’t get any of the alcohol on your clothes, and if you break a test tube you don’t get glass on you. Procedure: See-attached handout. Results: See chromatography with Audrey’s lab report....
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  • Liquid Chromatography 2 - 2701 Words
    FLinn SCIEnTIFIC Inc. "Your Safer Source for Science Supplies" p.o. Box 219 • Batavia, Illinois 60510 • 1-800-452-1261 • flinn@flinnsci.com • Visit our website at: www.flinnsci.com Liquid Chromatography AP Chemistry Laboratory # 18 Publication No. 10535A Catalog No. AP9093 Introduction In this experiment, liquid chromatography is used to separate the substances that are present in grape-flavored Kool-Aid®. First, the dyes responsible for the purple color, FD&C Blue #1 and Red #40 are...
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  • Thin Layer Chromatography - 771 Words
    (Author’s name) (Professor’s name) (Course details) (Date Abstract The aim of the lab was to separate and analyse analgesic drugs in a drug tablet. The method used to separate the components was Thin Layer Chromatography (TLC) with silica adsorbent as the stationary phase and 0.5% glacial acetic as the mobile phase. In one plate, five known samples were used as the reference, that is: Aspirin; Caffeine; Ibuprofen; and Salicylamide. Aspirin and Salicylamide were the only samples that...
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  • Thin Layer Chromatography - 1550 Words
    ------------------------------------------------- Thin layer chromatography From Wikipedia, the free encyclopedia Thin-layer chromatography | Separation of black ink on a TLC plate | Acronym | TLC | Classification | Chromatography | Other techniques | Related | Agarose gel electrophoresis SDS-PAGE | TLC of three standards (ortho-, meta- and para-isomers) and a sample Thin layer chromatography (TLC) is a chromatography technique used to separate non-volatile mixtures.[1] Thin...
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  • Column and Thin Layer Chromatography
    =========== ORGANIC CHEMISTRY ============ Organic Chemistry Laboratory - CH 201 (2010-2011) Experiment 4B Column and Thin-Layer Chromatography of Capsicum Frutescens L. Pigments John Cyril Abanto*, Vernalyn Abarintos and Clarice Gail Abella Department of Chemistry, College of Science University of Santo Tomas, Espana Street, Manila 1050 Date Submitted: September, 2010...
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  • chromatography lab write up - 1035 Words
     Separation Of A Dye Mixture Using Chromatography ********* AP Chemistry (3rd Block) Fauquier High School 8/ 31/ 13 ABSTRACT: The Rf values of seven known color dyes and three unknown samples were observed in order to compare results and discover the hidden identities of the three samples. Three solvents, H2O, Isopropyl alcohol, and a 2% NaCl solution, were introduced to the dotted chromatography strips to show three different ratios for each dye...
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  • Gas Chromatography Report - 1653 Words
     Gas Chromatography/Mass Spectrometry Susan Brown Keiser University Introduction GC/MS Gas Chromatography and Mass Spectrometry combines the identification power of gas chromatography with the quantitative analysis of mass spectrometry. Gas chromatography is a widely used laboratory technique that allows you identify specific parts of a mixture of substances. Mass spectrometry helps determine the molecular weight and components of the individual compounds. Where...
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  • Column and Thin Layer Chromatography
    COLUMN AND THIN LAYER CHROMATOGRAPHY Group 9 RAMOS, Sharina Joy; REYES Aina Marie; REYES Jallisa Maan; RUBIO, John Michael; SABINO Patricia Anne; SANTOS, Carlos Rafael ABSTRACT To separate the colored components of siling labuyo and to determine the purity of the components, the students performed a column and thin or solid-liquid chromatography procedure. The solid may be almost any material that does not dissolve in liquid phase. But for this experiment, the solid used by the students...
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  • Thin Layer Chromatography - 1909 Words
    Experiment 5 Title : Thin Layer Chromatography Objectives: i. To distinguish polar and non-polar solvents. ii. To familiar with the analysis technique by using the thin layer chromatography. iii. To differentiate the retention factor, Rf for different compounds. [pic] Result: |Compound |Distance traveled by the compound | |o-nitroanaline...
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  • Chromatography of Plant Pigments - 1376 Words
    CHROMATOGRAPHY OF PLANT PIGMENTS Marquez, Ma. Rica Paulene, Moises, Patrisha Kate, Policarpio, Jairus Paolo, Rolda, Zylene Joy Department of Biology, College of Science, University of the Philippines Baguio April 23, 2013 ABSTRACT The objective of this experiment was to apply the technique of paper chromatography as a method for separating individual plant pigments contained in plant tissue extracts containing pigment blends. The process of chromatography separates molecules because of...
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  • Size Exclusion Chromatography - 1278 Words
    Size Exclusion Chromatography Size Exclusion Chromatography (SEC) is the separation technique based on the molecular size of the components. Size exclusion chromatography is a kind of method to separate different size of molecules that put in solution. It was first discovered by two scientists who named Grant Henry Lathe and Colin R Ruthven. Both of them received the John Scott Award for this fabulous invention. There are various applications for Size exclusion chromatography such as...
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  • Liquid Chromatography Lab - 1733 Words
    Liquid Chromatography – Laboratory #18 Introduction: We are using liquid chromatography to separate the colored substances in grape-flavored drinks. We separate the component dyes, and then we separate the flavorings and citric acids. Background: Chromatography is a process that is used to separate a substance into its component parts. The separation occurs between the stationary and moving phase of the lab. The moving phase consists of a fluid and the stationary phase consists of a...
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  • Chromatography for Protein Purification - 825 Words
    Department of Chemical & Biomolecular Engineering THE NATIONAL UNIVERSITY of SINGAPORE Chemical Engineering Process Laboratory II Experiment B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein...
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  • Thin Layer Chromatography - 1909 Words
    Thin Layer Chromatography Submitted in partial fulfillment of the requirements for: CHE 324 Organic Chemistry Laboratory Dr. Robert Duncan Fall Semester 2012 Caitlin Inman, Team Leader October 9, 2012 Tyler Byrd, Data Collection Shared Role, Technique Expert Introduction: “Chromatography is used to separate components of a mixture. For example, imagine a mixture of wood pieces, pebbles, and large rocks to be separated and the chromatography setup as a stream. Flowing...
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  • Chromatography of Commercial Analgesics - 1868 Words
    Chromatographic Analysis of a Commercial Analgesic’s Active Component I. Abstract This study on Chromatographic Analysis of a Commercial Analgesic’s Active Component seeks to separate, identify, quantify and analyze the active component found in Biogesic®, which may be useful for public awareness despite its wide acceptance and availability in the market. Furthermore, this study applies thin layer chromatography (TLC) technique since it is the simplest, cheapest yet most effective approach...
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  • Chromatography of Photosynthetic Pigments - 893 Words
    Chromatography of Photosynthetic Pigments Abstract In this experiment a process of chromatography was used to separate chlorophyll a, chlorophyll b, xanthophyll, and beta carotene. When these pigments were obtained they were used to measure the wavelengths by way of spectrophotometer of each and the total of all the pigments wavelength. Introduction Photosynthesis is a process by which plants use the sunlight to convert it from light energy into chemical energy. This equation shows us...
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  • Chem Lab Chromatography - 558 Words
    Gabriel Alizaidy Liquid Chromatography September 14, 2009 Objective: To separate the components of unsweetened, grape flavored Kool-Aid. Procedure: Using two syringes, inject different concentrations of alcohol to extract red and blue dyes, and artificial flavoring. Data: Conclusion: Different dyes are shown when separated by different concentrations of isopropyl alcohol. Discussion of Theory: Chromatography, resolution and selectivity played major roles in making the experiment...
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  • Separation of Ferrocene and Acetylferrocene by Chromatography
    Purpose 1. To prepare a dry-packed sample of product mixture of ferrocene and Acetylferrocene 2. To separate and purify the components in the product mixture by column chromatography. 3. To check the purity of the components by thin-layer chromatography (TLC). 4. To calculate the yield of acetylferrocene and the percent recovery of unreacted ferrocene. Table of Quantity showing various physical properties Type of substance Molecular Formula Molecular Weight (g/mol) Density(g/cm3)...
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  • column and thin layer chromatography
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  • Dehydration of an Alcohol: Distillation and Gas Chromatography
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  • Chromatography: Color and Salt Solution Red
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  • Resolution of Matter into Pure Substances: Paper Chromatography
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  • Column and Thin Layer Chromatography: the Separation of Spinach Pigment
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  • Lab report for chromatography,international baccalaureate programme
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  • Analysis of Analgesic Tablet by High Performance Liquid Chromatography
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  • Alkenes from Alcohols: Analysis of a Mixture by Gas Chromatography
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  • High Performance Liquid Chromatography Analysis of Aspirin
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  • Thin Layer and Column Chromatography: Extracted Total Lipids of Chicken Egg Yolk
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  • Chromatographic Separation - 656 Words
    Chromatographic Separation of Sodium Fluorescein and Methylene Blue In this two-part lab the students will: • Learn the techniques associated with thin layer and column chromatography. • Determine the effects of the stationary phase in separating a mixture by chromatography. • Determine the effects of the mobile phase in separating a mixture by chromatography. • Conduct a separation using column chromatography. In the first week, the students will determine the best solvent system...
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    Band broadening theory (Van Deemter equation) It is well recognized now that column band broadening originates from three main sources: 1. multiple path of an analyte through the column packing; 2. molecular diffusion; 3. effect of mass transfer between phases. In 1956 J.J. Van Deemter introduced the equation which combined all three sources and represented them as the dependence of the theoretical plate height (HETP) on the mobile phase linear velocity. Originally, it was introduced for...
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  • Difference in Lymphatic Function in Health and Disease State
    QUALITY EVALUATION OF PARACETAMOL IN THE BULK, DOSAGE FORMS AND BODY FLUIDS USING THE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) TECHNIQUE S. Asare-Nkansah and J.K. Kwakye Department of Pharmaceutical Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana ABSTRACT High Performance Liquid Chromatography has been used to evolve an analytical procedure for the evaluation of the content of paracetamol in the bulk,...
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  • instrumental analysis chemistry Study guide
    Beam Chopping corrects for flame emission but not for scattering. Can distinguish the signal of the flame from the atomic line at the same wavelength. The higher the absorbance the higher the transmittance. Intro to IR Near IR- (0.78 to 2.8 micrometers) Mid IR- (2.5- 50 micrometers) Far IR- (50-1000 micrometers) Most common region used is Mid IR btwn 2.5- 15 micrometers Mid IR: based on diffracting gratings but now current instrumentation are of Fourier transform type Far-IR:...
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  • TLC analysis - 1465 Words
     International Islamic University Malaysia, Kuantan campus DEPARTMENT OF BIOTECHNOLOGY KULLIYYAH OF SCIENCE SBT 4534 ANALYTICAL METHODS FOR FUNCTIONAL FOODS & NUTRACEUTICALS LABORATORY REPORT LAB 2: FAT & OIL FROM OIL-SEEDS: PART II THIN LAYER CHROMATOGRAPHY ANALYSIS (TLC) COORDINATORS: ASSOC. PROF. DR. JAMALUDDIN BIN MOHD DAUD ABDUL AZIM ADNAN 1016741 INTRODUCTION TLC chromatography or thin layer chromatography is a type...
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  • Forensic Science Lab Questions
    1. What physical evidence did you find at the victim’s home? A box of matches, a space heater, beer bottles, Natalie’s wallet, a V-shaped burn pattern, burned outlet, a protected surface on the ground, crazed glass, and a puddle-shaped burn pattern. 2. Describe the process that you used to take a sample of the puddle-shaped burn pattern on the floor. Why did you also take samples from the portion of the floor that was not burned? First, I took the chisel to loosen up some of the wood...
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