Determining the Concentration of an Unknown Protein Solution with the use of the Bradford Method Paul Alcala‚ Andrea Basa‚ Melissa Caagbay‚ Frances Cayomba Department of Chemistry‚ University of Santo Tomas‚ Manila‚ Philippines Abstract The Bradford method used to determine the protein content of a certain solution (Menguito‚ 2010) and involves the acidic Coomassie Brilliant Blue G-250 as a coloring reagent. [1] The dye is originally pinkish-brown in color when it is in its acidic state
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Bradford Protein Assay Practical Report 1. Present your data (including raw data and calculated concentrations) for the protein standards in the form of a clear table. Give one example of how you calculated protein concentration. Do not forget a descriptive title and units (4marks) Title either too long or not descriptive or absent Your results are in duplicate & shouldn’t be referred to as ‘set1 & set2’ or ‘original’ and ‘duplicate’ Many of you think units of absorbance are nm but A has arbitrary
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Sam Bradford Samuel Jacob Bradford was born November 8th of 1978 to his loving parents‚ Martha and Kent Bradford. Sam is the only child‚ which allowed his parents to devote all their time to him and support what it is he wanted to do. Whether it was playing sports or the cello‚ his parents did what they could to develop it. They encouraged him in everything. "As long as it was a good‚ wholesome thing‚” Kent said. "If he wanted to do it‚ do it.” Even though Sam was central to their lives‚ he wasn’t
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Plymouth and Massachusetts Bay Colonies The Reformation was the driving force behind English Catholic dissenters‚ many of which would eventually form the base of groups heading for new lands to find religious freedom. These people would come to be called Puritans and their goal was to purify the Church of England. They wanted to do away with the “offensive” features such as Church hierarchy and traditional rituals of Catholic worship in order to promote a relationship between the individual and
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Man has been closely related with the nature since the down of civilization. Nature is helpful for all human beings. Nature abounds with trees. Trees are one of the best gifts of nature. As the civilization proceeds towards development‚ valuable nature assets are also getting damaged day by day. So it is the ease with trees. Now the ecological balance is distributed and the life becomes difficult. Our life and existence on earth greatly depends on trees. So we should plant trees through an existence
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Life on a Plantation The life on a plantation was different for different types of slaves. There were the Filed Slaves and House or Domestic slaves. Both slave types were treated very poorly and unfair. Their were severely beaten and punished‚ often for no reason. Female slaves were also often raped by their masters or by local village boys. They could not defend themselves because the punishment for that would be even more devastating. The slaves didn’t have comfortable cabins. They had to
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Myrtles Plantation Haunting Imagine being jolted awake in the middle of the night only to see a ghostly figure staring at you by the foot of your bed. This is what many people witness while staying at the Myrtles Plantation Bed and Breakfast located in beautiful St. Francisville‚ Louisiana (Nickell 12). The Plantation house is split into two parts‚ a museum and sleeping quarters for the brave guests who dare to stay the night (Rule 238). The Myrtles Plantation is known as one of “America’s Most
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Bradford Enterprises has experienced rapid expansion over the last 12 months‚ growing from 10 locations and 3‚000 employees in one state to 22 locations and 8‚000 employees in seven states. Bradford Enterprises has decided to upgrade its HR approach and raise it to a new level to be more competitive. The company’s owner is looking to implement three specific HR tools to help the HR do its job well and manage this expansion. The tools are selection tests‚ a Human Resource Information System (HRIS)
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arisen from the Myrtles Plantation. However‚ which are actually true? To understand some of the legends behind the plantation‚ you should understand the history behind it. The Myrtles Plantation is an antebellum plantation that was built around 1796 and 1797 by General David Bradford‚ also known as “Whisky Dave” in St. Francesville‚ Louisiana (Kermeen 43). Bradford lived there alone for a while. He then moved his wife‚ Elizabeth‚ and his five children to the Louisiana plantation from the northeast. Once
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emergence of the plantation system in the Caribbean. Discuss with special reference to the sugar industry. According to the Oxford Concise Dictionary a plantation is a long‚ artificially-established forest‚ farm or estate‚ where crops are grown for sale‚ often in distant markets rather than for local on-site consumption. The term plantation is informal and not precisely defined. Plantations are grown on a large scale as the crops grown are for commercial purpose Crops grown on plantations include fast-growing
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