is a method to measure how much a chemical substance absorbs light by measuring the intensity of light as a beam of light passes through sample solution. The basic principle is that each compound absorbs or transmits light over a certain range of wavelength. This measurement can also be used to measure the amount of a known chemical substance. Spectrophotometry is one of the most useful methods of quantitative analysis in various fields such as chemistry‚ physics‚ biochemistry‚ material and chemical
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investigated in a species of unicellular alga using the apparatus shown in Fig. 1.1. suspension of unicellular algae in water For Examiner’s Use 10.0 light of known wavelength oxygen probe magnetic stirrer Fig. 1.1 Two different strains of the species of alga were tested using a range of different wavelengths of light. • • Light of known wavelength was passed through the tube containing algae for two hours. The light transmission through the suspension and the oxygen concentration were then measured. light
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History of Pulse Oximetry Before the Pulse-Oximeter the only way to measure SpO2 was by using a painful arterial blood gas which took a minimum of 20-30 minutes to view the result. In 1974 Takuo Aoyagi a Niigata University student disclosed his first Pulse-Oximeter for the use of pilots in World War 2. He set about searching for a non-invasive way of monitoring arterial blood oxygen saturation. Nellcor’s pulse oximeter was put on the market in 1983 and this used his theory developed in 1974. By
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wave at about 300‚000 k/s. also known as the speed of light. The more energy the photons have‚ the brighter the light will appear. The energy of electromagnetic waves are measured in the electromagnetic spectrum‚ a big graph showing the different wavelengths and frequencies of electromagnetic waves. It ranges from radio waves‚ which are the weakest‚ to gamma rays‚ the strongest -Only a very small part of the spectrum is visible to the human eye‚ called the visible light spectrum. These colors are
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In this lab‚ we tested the effect of flame on different chemical solutions. In order to do this‚ we used a wooden stick soaked in a metal and nonmetal solution‚ hovered it over a bunsen burner‚ and recorded the color that the flame changed to. The seven solutions we tested were lithium chloride‚ strontium chloride‚ calcium chloride‚ sodium chloride‚ barium chloride‚ copper chloride‚ and potassium chloride. However‚ we had another four additional solutions‚ which had unknown chemical composition
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QUANTITATIVE DETERMINATION OF COPPER (II) CONCENTRATION BY SPECTROPHOTOMETRY D.DEL PRADO1‚ J. BELANO1‚ M.MAHUSAY2‚and M.FRANCISCO2 1 DEPARTMENT OF FOOD SCIENCE AND NUTRITION‚ COLLEGE OF HOME ECONOMICS 2INSTITUTE OF CHEMISTRY‚ COLLEGE OF SCIENCE UNIVERSITY OF THE PHLIPPINES‚ DILIMAN‚ QUEZON CITY 1101‚ PHILIPPINES DATE SUBMITTED: 12 MARCH 2013 DATE PERFORMED: 7 MARCH 2013 ------------------------------------------------- ------------------------------------------------- ABSTRACT -------------------------------------------------
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the reaction to completion. Wavelengths of 430 nm and 525 nm were chosen as dichromate absorbs best at 430 nm‚ while permanganate absorbs best at 525 nm. The concentrations of dichromate and permanganate in the unknown sample were 0.0122 M and - 1.94 x 10-3 M respectively. The negative value obtained for the permanganate ion concentration showed that the concentration was extremely low. Introduction: Spectrophotometry is the measurement of how different wavelengths of light are absorbed. Using
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and wavelength‚ intensity is a basic property of radiation. No natural object emits all of its radiation at just one frequency. Instead‚ the energy is often spread out over a range of frequencies. By studying the way in which the intensity of this radiation is distributed across the electromagnetic spectrum‚ we can learn much about the object’s properties. The Radiation Laws * Wien’s Law Tells us that the hotter the object‚ the bluer its radiation. Wien’s Law links the wavelength at which
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I. Our Senses Encode the Information Our Brains Perceive * Synesthesia- The perceptual experience of one sense that is evoked by another sense * For many years scientists dismissed synesthesia as rare curiosity or outright faking * Far more common than previously believed * Brain regions for different sensory modalities cross-activate one another * Sensation- Simple stimulation of a sense organ * Perception- The organization‚ identification‚ and interpretation of a sensation
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travel in waves. The difference in the colour depends on the wavelength of the light. Violet light has the shortest wavelength while red has the longest. There are other parts of the electromagnetic spectrum which includes infra-red‚ radar‚ television radio and micro- waves (past red on the spectrum)‚ and on the other end of the spectrum are the other invisible radiations‚ ultra- violet‚ X rays‚ micro waves and gamma rays. The wavelength of the light is important to the subject of the laser. A laser
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