The Virtual Lab – ELISA Test Lab: Immunology 09/04/2013 Instructors: Dr. Charlie Wilson Written by: Dipen Patel I. Objective: The purpose of the lab was to learn the procedure of performing an ELISA test to determine whether a particular antibody is present in a patient’s blood sample. ELISA is an abbreviation for “Enzyme-linked Immunosorbent Assay." II. Introduction: The interaction of antigen and antibody outside the body can be used to determine if patient
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solid? Liquid? Gas? Materials: Safety glasses‚ 10‚ 25 or 50 mL graduated cylinders Distilled water Metal Samples W‚ X‚Y & Z Procedure: 1. Class split into 5 groups. Every group will get data for four metals and tell it to the class 2. Choose a metal sample for every group then pass it around between groups. 3. Set a number of your metal sample and record the mass in the table.
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Biology Unit 2 Module 2 1.1: Explain the uptake of ions by active transport in roots; * Emphasize the role of the endodermis. Most plants secure the water and minerals they need from their roots. The path taken is: soil -> roots -> stems -> leaves The minerals (e.g.‚ K+‚ Ca2+) travel dissolved in the water (often accompanied by various organic molecules supplied by root cells).Less than 1% of the water reaching the leaves is used in photosynthesis and plant growth. Most of it is
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Potato Catalysis Lab Pre-Lab Assignment Background Information: An enzyme is catalytic protein. It is the most important type of molecule found in living cells. Cells would not be able to function without enzymes. Enzymes speed up or slow down chemical reactions of the cells. It is usually easy to identify the names of enzymes because they end in -ase. The enzyme that acts upon the substrate hydrogen peroxide is usually called catalase. This enzyme is found in both plants and animals
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Describe their role in the process. Pollen is produced in the stamen. Pollination occurs when that pollen is transported from the anther to the style by insects or animals that are attracted to the plant by the beautiful petals of the flower. 2. Which parts of the flower are involved in fertilization and fruit development? Fertilization starts after pollination has occurred‚ and begins inside the pistils. The ovule contained inside the ovary is fertilized and the ovule begins to harden and
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Molecules of Life 4 Critical large Molecules- 1.) Carbohydrates 2.)Lipids 3.)Proteins 4.) Nucleic Acids -On the molecular scale‚ members of three of these classes--- Carbohydrates‚proteins‚ and nucleic acids--- are huge and therefore called Macromolecules. - Architecture of a large biological molecule helps explain how that molecule works. ---------------------------------------------------------------------------------------------------------------------------- Macromolecules
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Experiment #1: Carbohydrate Digestion • Tube 1 Digestion Lab – 3 ml water • Tube 2 – 3 ml 0.2% amylase • Tube 3 – 3 ml 0.2% amylase + 10 drops of 1.0M HCl • Tube 4 1 2 4 3 – 3 ml 0.2% amylase – place in hot water bath for 5 min Experiment #1: Carbohydrate Digestion • Add 5.0 ml starch solution to each tube • Incubate in 37°C bath for 1.5 hr • Divide contents of each tube evenly into 2 tubes – Lugol’s Test – Benedict’s Test Experiment #1: Carbohydrate
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thickening for soups and sauces‚ in jellies and ice cream‚ in cosmetics‚ for clarifying beverages‚ and for sizing fabrics. Agar is also used in medicines to promote peristalsis and relieve constipation and is resistant to a breakdown by bacterial enzymes. Sodium carbonate‚ Na2CO3‚ also known as washing soda or soda ash‚ is a sodium salt of carbonic acid. It can be extracted from the ashes of many plants and has a cooling alkaline taste. It is one of the most basic industrial chemicals. The acid’s
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Unit 2 Home Labs - KEY Goals: Read Safety Perform Changes: Physical or Chemical Lab Perform Special Physical Properties of Matter Lab Perform Paper Chromatography Lab Turn-in: 1) Changes: Physical or Chemical Lab - Completed Procedure / Observations‚ questions‚ and a written description of most important concept that you learned in the discussion section. 2) Special Physical Properties of Matter Lab – Completed Procedure / Observations‚ questions
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Environment Can Effect Enzymes Introduction: In a chemical reaction there sometimes can be a catalyst present known as an enzyme. An enzyme is a protein that speeds up a chemical reaction by lowering the amount of activation energy required to start the reaction. By lowering the activation energy‚ more of the substrate is able to participate in the reaction‚ speeding it up. Enzymes are substrate specific. The substrate is what the enzyme bonds to. That is to say that enzyme A will only react with
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