Enzyme assay lab report Health and safety: 4-nitrophenol is harmful. Introduction: Enzymes are quaternary structured proteins that are specific biological catalysts that speed up a reaction without being used up. They contain an active site that allows substrate to bind to a specific area on the enzyme which is of a complimentary shape of the substrate. There are two models of enzyme action‚ the Lock and Key model and the Induced Fit model. The Lock and Key model states that the enzyme has a specific
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Enzymes and ATP Enzymes act as protein catalysts in biochemical processes Enzymes bind to a substrate and forms the enzyme substrate complex. Enzymes work by lowering the energy of activation. Activation energy must be supplied for the reaction to begin‚ once supplied‚ the reaction can proceed on its own. Enzymes can speed up events. They are not used by during the reaction because the enzyme stays the same‚ it does not change during the reaction. (Hudon-Miller‚ Enzymes‚ 2013) Enzymes act as
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(Figure 2.) Relative Tooth Width (mm.) Results: The dental formula is a convention used by mammalogists to determine the tooth number and tooth arrangements. The dental formula is calculated by counting the number of incisors‚ canines‚ premolars‚ and molars present in the upper jaw (one side) and counting the number of incisors‚ canines‚ premolars‚ and molars in the lower jaw (one side). The total number of incisors‚ canines‚ premolars‚ and molars (upper +lower jaw) multiplied by 2 would equal
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stock solution of glucose‚ water‚ 6 test tubes‚ ruler‚ water bath‚ solution of unknown concentration (Sample A)‚ Benedict’s reagent METHOD: 1. Solutions of different concentration were made from the stock solution and placed in test tubes. 2. All test tubes were labeled appropriately‚ according to table below‚ and Benedict’s test was carried out on the various dilutions for 5 minutes. 3. The colours of the solution were observed and heights of precipitate formed were recorded. The test
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Enzymes INTRODUCTION Enzymes are protein cells and they are found in the gut. They help to break down the food you eat. Enzymes are produced by living cells and they speed up reactions because they are biological catalysts. If we did not have enzymes‚ the reactions in our cells would be too slow to keep us alive. This means that they are extremely important for our survival. The properties of enzymes are: ⋆ They can be used many times‚ because they can speed up reactions without
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Environmental Parameters of Enzyme Activity Alex Rocha Texas State University Abstract If you’ve ever left a cut up apple out for long‚ you’ll notice that after a while‚ it will turn brown. The reason for this is an enzyme named catechol oxidase‚ a ubiquitous plant enzymes containing a dinuclear copper center (Klabunde‚ Eicken‚ Sacchettini‚ & Krebs‚ 1998). In this experiment‚ we used two different chelators‚ ethylene diamine tetraacetic acid and phenylthiourea to test which would stop
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Factors Affecting The rate of Enzyme Activity Prediction: As the temperature increases the rate of enzyme activity will also increase‚ thus increasing the rate of reaction. However‚ if the temperature is too high the enzyme will denature. Materials: 4 test tubes 2 small beakers A dozen filter paper disks Test tube rack Hydrogen peroxide (H2O2) Potato extract Forceps Thermometer Hot plate Large beaker Ice cubes Graduated cylinder Stopwatch Procedure: Step 1 Place 10 mL of potato
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Erin Arroyo Lab report June 11‚ 2013 Biology 123 Professor K Title: Scientific Investigation of the Peroxidase Enzyme & Temperature Abstract: In this lab we tested the effect temperature has on the rate of enzyme activity. The way we figured this out was by taking four different temperatures and testing the different absorbance levels they produced every 20 seconds for two minutes straight using a spectrophotometer. The important part of this experiment was the temperature
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Biology lab AIM: To test a leaf for starch APPARATUS/MATERIALS : * bunsen burner‚ tripod stand‚ wire gauze(OR 90oC electric water bath OR hot plate) * 250 cm3 beaker * boiling tube * anti- bumping granules * forceps * test tube holder * white tile * leaf to be tested (hibiscus leaves are excellent) * 90% ethanol * iodine/potassium iodide solution PROCEDURE: The video above shows the steps in Testing a Leaf for the Presence of Starch. The Procedure outlined
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Dana Calderone Responses of Enzyme Activity from pH and Concentration Abstract Enzymes are the key to many of the chemical reactions that our bodies depend on to live. Without enzymes‚ we would not exist. These biological catalysts speed up the reactions as well as reduce the amount of activation energy needed to complete the process. Knowing how important enzymes are to us‚ it is important to realize what they require to function. They need select conditions and rates to work right. These conditions
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