-13µl ammonia is added‚ then vortex. -Complexing reagent is added (0.8ml)‚ then vortex. Solid phase extraction (SPE) MET & Cat: -Take 1ml methanol. -1ml ammonia buffer (0.2M) is added. -The sample is added. -1ml of ammonia buffer + MeOH is added. -Dry using the centrifuge at high speed for 2 to 5 mins.
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solvent should be high at high temperature & low at room temperature. If possible‚ the impurities should be insoluble in the solvent. 2.> How were soluble impurities removed from the derivative? [ANS] By soaking the impure derivative in 1 cm3 of methanol and resume suction. Repeat this for about 2 times. 3.> In the recrystallization procedure‚ why were the crystals dissolved in only the minimum amount of
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experiment synthesized H-Gly-Phe-OH dipeptide using “Fmoc chemistry”. The first part of experiment was the synthesis of L-phenylalanine methyl ester hydrochloride. The methyl ester can be synthesised by reaction of thionyl chloride‚ SOCl2 and dry methanol with L-phenylalanine under reflux condition. The peptide bond was formed later in the experiment‚ where HBTU‚ DiPEA and a solution of Fmoc-Gly-OH in DMF were added to a solution of L-phenylalanine methyl ester hydrochloride in DMF. The product formed
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biological methods. The Chrysophyllum canito leaf extracts were used for silver nanoparticles synthesis. In this study utterly revealed the best milli molar concentrations of silver nanoparticles synthesis in diverse solvents such as aqueous‚ ethanol‚ methanol‚ chloroform and petroleum ether‚ but the excellent silver synthesized solvents only chosen for further more characterization studies. The aqueous and ethanol solvents sample and chemically synthesized oxalic acid silver synthesis got excellent
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Aldehyde and Ketone 1. ALDEHYDE Definition: An aldehyde is an organic compound containing a formyl group. This functional group‚ with the structure R-CHO‚ consists of a carbonyl center (a carbon double bonded to oxygen) bonded to hydrogen and an R group‚ which is any generic alkyl or side chain. The group without R is called the aldehyde group or formyl group. Aldehydes differ from ketones in that the carbonyl is placed at the end of a carbon skeleton rather than between two carbon atoms
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obtained. 3. Step 1 and 2 are repeated for three times to make sure the purple or pink solid is obtained. (C) The cis to trans isomerisation 1. A solution of (0.02M; 14mg in 25ml) of the trans isomer and cis isomer is prepared in methanol and its UV-visible spectrum is recorded. 2. The solution is placed quickly and tightly stoppered into a constant temperature bath at 35 °C 3. The most
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CHAPTER I THE PROBLEM Introduction In the Philippines‚ soy beans are enjoyed most in the form of popular street snack called taho. This yogurt-like concoction is similar to fresh tofu as both are eaten with spoons. Taho is served by street vendors who carry shiny tin containers that are balanced on both ends of a wooden plank. One can hold the hot taho‚ which is scooped into a cup. The other can hold the caramel sauce and tapioca which is mixed in the cup. These vendors travel the residential
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seem to go wrong in the triangle. One argument researchers show is that the location of the triangle is in the deepest part of the Atlantic Ocean at 30‚000 feet. Since the region spans over the deepest part‚ it contains more methanol. The gas bubbles produced by the methanol makes the water unstable‚ therefore the water could not support a ship like it should. In support of the way planes disappear‚ scientists say that the coordinates of the triangle fall directly on the equator. That fact makes
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cold temperatures and it is harder to travel through the tubes(high viscosity) while hydrogen doesn’t solidify . * Biodiesel releases the most C02 out of all the fuels. Hydrogen releases water vapor * The range for biodiesel cars is 10% less than propanol cars . Hydrogen cars have 25% more range than biodiesel cars and 15% more than propanol cars. Hydrogen VS propanol: * Propanol has problems in high climates due to viscosity and the fuel solidifying while hydrogen cars don’t have problems
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UNIVERSITY OF THE GAMBIA LECTURE NOTES COURSE: PRINCIPLES OF CHEMISTRY II (ORGANIC CHEMISTRY) CODE: CHM 161 2ND SEMESTER SESSION: 2012/2013 LECTURER: ANTHONY F. ADJIVON UNIT 1 INTRODUCTION Organic chemistry started as the chemistry of life‚ when that was thought to be different from the chemistry in the laboratory. Then it became the chemistry of carbon compounds‚ especially those found in coal. Now it is both. It is the chemistry of the compounds of carbon along with other elements
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