3.6.5. Southern hybridization Southern blot hybridization analysis was carried out using standard procedures (Sambrook et al. 2001). Approximately 20 µg of total genomic DNA from transgenic plants and untransformed control plant was digested with EcoRI‚ and then separated by electrophoresis on 0.8% agarose gel. Digestion profile was checked with 1µg DNA‚ with single cutter enzymes EcoRI (10U) in T-DNA region. For southern experiment‚ 20µg DNA was digested overnight. Restricted fragments were separated
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In 1788 the first fleet disembarked on the shores of Botany Bay. Shortly thereafter Australia became the first colony founded entirely upon the work of convicted felons. The traditional interpretation of the Australian colonies is that‚ it was a period of harsh and brutal forced labor‚ where convicts were treated as human commodities and labor was extracted by punishment. Convicts were subjected to various types of reprimands such as shortened rations‚ leg-irons‚ being placed on treadmills‚ head
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The Truth of a Southern Dream In Fitzgerald’s "Ice Palace"‚ Sally Carrol Happer is a Georgia girl tired of the lazy and unambitious South. She desires to break free from her hometown of Tarleton‚ captivated by the promises and new opportunities in the North. Her fiancée Harry is supposed to be the fiancée that can provide her this new lifestyle‚ but her romanticized vision becomes disillusioned. Sally Carrol’s misconception of the shimmering North is captured after experiencing the reality of
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The development of the American colonies had six different factors contributing to it. They were the Enlightenment‚ European population explosion‚ Glorious Revolution‚ Great Awakening‚ mercantilism‚ and Religious tolerance. The Enlightenment was a cultural movement that challenged the authority of the church in science and philosophy while elevating the power of human reason. One of the most influential Enlightenment writers was John Locke. He argued with the church that people were not
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Southern Discomfort April 20‚ 2013 Michael Miklus Upper Iowa University Case questions for the Southern Discomfort 1. Should the company go ahead with the move to Mexico or try one more time to work things out in the Oconomo facility? Support your position. Because of the long history / relationship with the local community in Oconomo the company has the responsibility of attempting one last time to work things out with the local union. If‚ after the last attempt
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“Child labor” By Elie Bou Chaaya ENL 110 Section “D” Miss Zeina Fayyad 18‚ January‚ 2008 Outline: Thesis statement: Child labor nowadays is considered to be a social injustice due to its harmful effects on the personal and psychological life of the child where the kinds of work offered to the child increases the amount of these effects regarding the solutions. I. Harmful personal and physical effects of child labor A. Low pay B. Very long work hours C. Forbids children
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omnipresent‚ invisible universal force‚ pertaining to the "three ’life crises’ of birth‚ puberty‚ and death"‚ spirits‚ visions‚ the shaman and communal ceremony.[1] After European settlement‚ religious history began more than a century before the British colonies became the United States of America in 1776. Some of the original settlers were men and women of deep religious convictions. The religious intensity of the original settlers diminished to some extent over time but new waves of 18th-century immigrants
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Hershey Sued Over African Cocoa Farm Child Labor Accusations An investor group is suing the United States largest chocolate manufacturing company Hershey Co.‚ for allegedly using cocoa that is the product of child labor. The Louisiana Municipal Police Employee’s Retirement System filed a lawsuit in October of 2012‚ which seeks to force Hershey to open its corporate records for investors. This public pension fund‚ made up of Hershey shareholders believes that records could reveal the leading U
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Definition Labor is a physiologic process during which the products of conception (ie‚ the fetus‚ membranes‚ umbilical cord‚ and placenta) are expelled outside of the uterus. Labor is achieved with changes in the biochemical connective tissue and with gradual effacement and dilatation of the uterine cervix as a result of rhythmic uterine contractions of sufficient frequency‚ intensity‚ and duration.1‚2 Labor is a clinical diagnosis. The onset of labor is defined as regular‚ painful uterine contractions
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the seventh century‚ Europeans established colonies in North America. The English colonies were originally established because proprietors from England were granted charters to settle and govern lands. Other European colonies were established around trading posts. Over time‚ the English gained control of the thirteen colonies through force or purchase; eventually‚ by regions were known as the Southern‚ Middle and New England colonies. Although the colonies were under the control of the English and
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