Trevor Smith Biol 4400 Dr. Achberger May 8‚ 2013 a. From the amino acid sequence the gene is the LacI repressor for Escherichia coli. See appendix 1 for the search result. b. The complete nucleic acid sequence of the protein with the open reading frame (ORF) indicated by red and green colorization as well as the forward and reverse primers underlined (a screenshot was taken so the sequence could be clearly displayed): c. The ORF and flanking nucleotide sequences are displayed in part “b
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1 Molecular-Orbital Theory Introduction Orbitals in molecules are not necessarily localized on atoms or between atoms as suggested in the valence bond theory. Molecular orbitals can also be formed the LCAO where more than two atomic orbitals are used. (Technically‚ we can use all of the atomic orbitals in the LCAO.) Linear combinations of orbitals result in the interference of waves. Both constructive and destructive interference may result. Within a diatomic molecule‚ N atomic orbitals
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THE MOLECULAR BASIS OF INHERITANCE I. History A. Discovery of “transformation” – a change in genotype and phenotype due to the uptake of external DNA by a cell 1. Griffith 1920s did experiments with Streptococcus pneumoniae (p294 fig16.2) a. took two strains of S. pneumoniae‚ one virulent‚ one not b. heat killed virulent strain‚ then mixed them with the living nonvirulent strain c. living nonvirulent strain became virulent d. nonvirulent strain took on virulent strain’s DNA became virulent
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Ultra thin film coatings can be used in a wide range of applications as lubricants‚ anti-stiction layers‚ molecular glues‚ coupling agents or adhesion layers. The mentioned molecular coatings are used to modify material surface properties. The traditional coating deposition methods consist of a set of sequential wet baths. The wet processing method is complex and limits the ability of molecular coating in many MEMS applications. The use of liquid precursors has extremely limited bath life‚ which cause
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Module 1 ‘Introduction to Molecular Biology’ Learning Objectives (Chapter 6) Molecular Biology‚ DNA‚ chromosomes‚ and the central dogma. Understand Nucleic Acid Structure and properties (including alternate forms of DNA structure and supercoiling‚ and renaturation) Explain the nature of Prokaryotic Chromosome Understand the differences in re-association kinetics of single-copy vs. repeated sequence Understand how DNA re-association provides a measure of genome size in base pairs Functions
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I. Purpose The purpose of this lab is to determine the molecular mass of a volatile liquid. II. Procedure • Place a cork with a small hole in it inside of a test tube and find the weight of the two together using a sensitive balance. • Pour 0.5 mL of the unknown volatile liquid into the test tube‚ insert the cork‚ and place the tube into boiling hot water while keeping the cork above water level. As the gas evaporates‚ excess gas will be released throughout the whole in the cork. Keep the test
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Assess the strengths and weaknesses of using unstructured interviews to investigate violent crime. (15 marks) Violent crime is difficult to study in the context where it occurs; this is because by their nature‚ violent crimes are often swift and unexpected. It is very difficult‚ if not impossible‚ for a researcher to know when and where a violent crime is likely to be committed. Unstructured interviews are a research method which entails a sociologist asking participants a series of open-ended
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mechanisms that regulate cellular senescence‚ organismal ageing‚ and species-specific lifespan depend on a synergy of pathways that are multifactorial and extremely complex‚ though not yet completely understood. Recently‚ the development of new molecular techniques has elucidated‚ at least in part‚ the primary pathways involved in ageing. In parallel with the search to uncover the factors that control ageing is the endeavor to discover methods of extending lifespan‚ in hopes of living both youthfully
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Empirical and Molecular Formula | Key Concepts * Empirical Formula of a compound shows the ratio of elements present in a compound. * Molecular Formula of a compound shows how many atoms of each element are present in a molecule of the compound. * The empirical formula mass of a compound refers to the sum of the atomic masses of the elements present in the empirical formula. * The Molecular Mass (formula mass‚ formula weight or molecular weight) of a compound is a multiple of the empirical
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Molecular Biology 344 fall 2012 A Johnson Due 9/11/12 as hard copy at the beginning of lecture Problem Set 1 1. Look carefully at the structures of the two molecules shown below. a. What would you expect to happen if you added ddCTP (shown in a) to a DNA synthesis reaction in vitro in large excess over the concentration of deoxycytidine triphosphate (dCTP)? How will this affect the pattern of bands on the sequencing gel? The ddCTP lacks the OH‚ which is required for elongation
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