Staphylococcus epidermis (gram positive) bacteria for 24 hours (in an incubator)‚ garlic will have the strongest antimicrobial effect. This is because of allicin (an active principle of garlic homogenates)‚ which has a chemical reaction with thiol groups of enzymes in the bacteria. Ginger will have the least antimicrobial effect. This is because there are not as many chemical reactions with enzymes in the bacteria. Risks: The risks of using Staphylococcus epidermis bacteria to test in microbiology are few
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Chemical T ests for Unknowns Chemical tests have been developed as a means of identifying what functional groups are present in an unknown compound. Since IR and NMR spectroscopy has been developed‚ they are no longer critical to unknown analysis‚ but they can still be useful for confirming what you have determined by spectroscopy. Some of them look cool too! In order for a chemical reaction to work as a chemical test‚ it must 1) create a visible result (a color change‚ a precipitate‚ etc)
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LAB 5: Coliform bacteria in surface waters I. Introduction Many organisms‚ including humans‚ have symbiotic bacteria in their guts that aid digestion. Symbiosis is an intimate relationship between different organisms in which both the host organism‚ e.g. the human‚ and the symbiote‚ e.g. bacteria‚ benefit from each other. In this case‚ the bacterium gets a favorable environment and food source in the intestines of a human. In return‚ these bacteria improve the digestibility of food through
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The melting point range is defined as the span of temperature from the point at which the crystals first begin to liquefy to the point at which the entire sample is liquid. This data can be tabulated experimentally through multiple trials for an unknown and referenced against the chemistry literature for a known compound. In Macroscale and Microscale Organic Experiments‚ 5th ed‚ Williamson notes that most pure organic solids will melt repeatedly over a narrow temperature range of 1°C. As a measure
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Determining the unknown concentration of HCl via heat of neutralization of a reaction involving strong electrolytes and weak electrolytes. Myeongwon Lee 20522885 Partner: Frank Wong TA: Afsoon CHEM 120L – Earth Science & Chem 149 Section: 004 Tuesday‚ November 26th‚ 2013 Introduction All chemical changes are accompanied by change in energy and this energy is in form of heat. The energy change of a reaction that happens at constant pressure is defined as heat of reaction or enthalpy change and the
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Jennifer Hauss March 4‚ 2015 Bacterial Transformation Lab Report Introduction In this lab‚ the goal was to transform the bacteria e-coli to glow in the dark (or under a black light). Four plates were set up with agar in them for the bacteria to feed on and grow. Changes were then made to the bacteria. One plate was the control plate‚ having only the LB or agar for the bacteria and negative pGLO‚ which is the liquid not containing the plasmid. This is the plate that was compared with the three others
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TITLE : * Analysis of an unknown acetic acid solution OBJECTIVES : * To prepare the sodium hydroxide solution‚ NaOH * To standardise the base against potassium hydrogen phthalate * To analyse the unknown acetic acid RESULTS : A. Preparation of the sodium hydroxide solution Volume of NaOH taken from the stock solution = 3.33 mL B. Standardisation of the base against potassium hydrogen phthalate | 1 | 2 | 3 | Weight KHP | 1.0000 | 1.0004 | 1.0006 | Final volume
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performed this test‚ the initially slant for unknown microorganism #17 is important. For this lab‚ two identical slants are used for two reasons. Firstly‚ the slant can be used to make sure that there is no contamination from the Nutrient Agar plate. Secondly‚ the second slant will become a stock culture to prevent the shortage of slants during performing the series of tests. Kliger’s Iron Agar tests can be used to determine multiple characteristics of unknown microorganism #17. Kliger’s Iron Agar slants
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Lab # 11 – Acid - Base Titration Introduction: The purpose of this lab is to determine the molarity (M) of an unknown HCl solution. A NaOH solution will be made and its molarity calculated. A sample of the NaOH solution will be titrated against the unknown HCl solution to calculated the volume needed to neutralize it. With these volumes the unknown molarity can be calculated. Theory: Solutions are made up of solvents and solutes. Materials known as acids when dissolved
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Grainy and shiny white Becoming all liquid 125 Clear B. Melting point of unknown #42 1st trial Temperature (¡ÆC) Observation Unmelted Shiny light yellow crystal First liquid appear point 99 Grainy yellow Becoming all liquid 101 Clear 2nd trial Temperature (¡ÆC) Observation Unmelted Shiny light yellow crystal First liquid appear point 95 Grainy yellow Becoming all liquid 97 Clear C. Melting point of mixture (Unknown + Benzil) Temperature (¡ÆC) Observation Unmelted Shiny light yellow
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