bleaches and cleansing wounds. In a cell its build up would be highly toxic. However‚ liver cells contain an enzyme‚ catalase‚ which immediately breaks down hydrogen peroxide. It is a peroxidase and breaks up the toxic hydrogen peroxide to water and oxygen which are both nontoxic. The reaction is exothermic‚ meaning that energy is released in the form of heat. It is the fastest known enzyme. Hydrogen peroxide Water + Oxygen 2H2O2 2H2O O2 List of materials: Safety glasses Iron powder in a watch
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The mean data observed show a number of trends. The average weight and length tend to decrease as the amount of Ammonium Sulfate approaches the full recommended dose. On the other hand‚ the average amount of protein per gram of tissue tends to increase as the amount of Ammonium Sulfate approaches the full recommended dose. Our null hypothesis was that there is no difference between the mean weights or the mean lengths of the seedlings two different treatments. (A+B‚ A+C‚ and B+C) Each of these
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in an enzyme catalyzed reaction? Hypothesis: Increased pH substance will increase the rate of reaction Table: Table 1: Baseline of peroxide Time (min) Qualitative color 1:00 5 2:00 6 3:00 7 4:00 8 5:00 9 Table 2: effect of increased pH on peroxidase Increased pH by 5mL Color intensity 2 10 5 10 1 10 8 9 11 10 3 10 Time in minutes Graph: Graph1: The effect of pH on the enzyme Peroxidase
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REVIEW SHEET‚ EXAM 3 Chapter 6‚ Nutrition and Microbial Growth Define the four major modes of nutrition in microorganisms and distinguish among them in terms of energy and carbon sources The four major modes of nutrition are: 1. Photoautotrophs: (ex. Plants‚ some protozoa‚ & alegae) -microorganism which use carbon dioxide as a carbon source and light energy from the environment to make their own food. 2. Chemoautotrophs: -microorganism which uses carbon dioxide as a carbon source and
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added at an appropriate dilution and incubated with the membrane. Antibodies present in the serum bind to the protein(s). In order to detect the bound antibodies‚ anti-immunoglobulin antibodies coupled to an enzyme alkaline phosphatase or horseradish peroxidase are added. This anti-IgG enzyme is commonly called a "second antibody" or "conjugate". Finally‚ after excess second antibody is washed free of the blot‚ a substrate is added which will precipitate upon reaction with the conjugate resulting
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Brassica rapa var rapa (Man Jing Turnip) is an important biennial‚ outcrossing‚ mesopolyploid herbaceous plant in the family Brassicaceae‚ native throughout Europe‚ Russia‚ Central Asia and the Near East (Prakash & Hinata 1980 ) with Europe proposed as one centre of origin.. In the alpine regions of the Qinghai-Tibetan Plateau it was widely used as a food source‚ when other crop production was limited approximately 2000 B.C. These highlands range in altitude between 3000-4000 m and are characterized
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jar is not an oxygen free system. This is the best method of culturing a microaerophile. An obligate anaerobe is a microbe that cannot tolerate oxygen and will be killed in its presence. This type of organism is killed because it lacks the enzymes necessary to eliminate toxic oxygen products. An obligate aerobe is a bacterium that requires atmospheric levels of oxygen for normal growth. A facultative anaerobe is a microorganism that can grow with or without oxygen but usually grows
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hydrogen peroxide at 240 nm according to Aebi [25]. Superoxide dismutase (SOD) activity was assessed by pyrogallol autoxidation according to Marklund [26]. Reduced glutathione (GSH) concentration was measured according to Ellman [27]. Glutathione peroxidase (GSH-Px) activity was determined in accordance to procedure described by Beutler [28]. Isolation of mitochondrial fraction from kidney tissues Briefly‚ a portion of the fresh kidney tissue was homogenized in isolation buffer [250 mM Sucrose‚ 1
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Introduction : Solanum nigrum Linn. commonly known as Black Nightshade is a dicot weed in the Solanaceae family. Other names of S. nigrum are European Black Nightshade or just black nightshade‚ Duscle‚ Garden Nightshade‚ Hound’s Berry‚ Petty Morel‚ Wonder Berry‚ Small-fruited black nightshade. It is a species of the Solanum genus‚ native to Eurasia and introduced in the Americas‚ Australia‚ Asia and South Africa. The plant is cultivated as a food crop‚ both for its fruit and its leaves in some
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450nm‚ which was proportional to laccase activity (Parshetti et al.‚ 2011). The activity of Lignin peroxidase was determined by monitoring the conversion of propanaldehyde at 300 nm for 30 min in a reaction mixture of 10µl enzyme extract and 100µl containing 100mM n-propanol‚ 250 mM tartaric acid and 10 mM H2O2 (Kalyani et al.‚ 2008). The enzyme activities were measured at room temperature. One enzyme unit was defined as an activity producing 1µmol product per min (Laccase) or consuming 1 µmol substrate
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