GRAM STAIN THEORY: The most widely used staining procedure in microbiology is the Gram stain‚ discovered by the Danish scientist and physician Hans Christian Joachim Gram in 1884. Gram staining is a differential staining technique that differentiates bacteria into two groups: gram-positives and gram-negatives. The procedure is based on the ability of microorganisms to retain color of the stains used during the gram
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series of proportional amounts. Dilutions are usually made in multiples of 10. (3) The viable plate count procedure allowed for live cells in the milk samples to be analyzed. The raw milk (unpasteurized) sample demonstrated too many colony
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Objectives 1. To determine biochemical activities of microorganisms. 2. To observe the product of biochemical activities of microorganisms. 3. To learn the skills of inoculation agar tubes and agar plates. Introduction Microorganisms are able to carry out different biochemical activities with the ease of different enzymes. Each of these enzymes carries out one specific type of the chemical transformation. They convert substrates into product. A) Carbohydrates Fermentation Microorganisms utilize
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In the experiment‚ the different growth phases were observed through the analysis of the absorbance of broth with inoculated organism (E.coli). There was no viable cell counting done with the plates because of the failure to count and record the number of viable cells after a 24-hour incubation period. The measurement of absorbance may be used to analyze bacterial growth since the absorbance or the measure of the turbidity is directly proportional
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agent of a disease in a patient‚ so as to know how it can be treated‚ to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium. Broth culture #72 was randomly selected and subjected to qualitative tests for identification. It is suggested that culture #72 is an example of Serratia marcescens and Micrococcus
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References: Bates‚ S.T. and Cropsey‚ G.W. “Bacterial Communities Associated with the Lichen Symbiosis.” Applied Environmental Microbiology‚ 2011‚ vol. 77‚ Issue 4‚ pp 1309-1314. Jobaggy‚ E.G. and Jackson‚ R.B. “The distribution of soil nutrients with depth: Global patterns and the imprint of plants.” Biogeochemistry‚ 2001‚ vol. 53‚ pp 51-77. Pace‚ Norman R.‚ “A Molecular View of
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groups of enzymes in the bacteria. Ginger will have the least antimicrobial effect. This is because there are not as many chemical reactions with enzymes in the bacteria. Risks: The risks of using Staphylococcus epidermis bacteria to test in microbiology are few. Gloves must be worn while handling Staphylococcus epidermis to reduce any risk of spreading the bacteria. Regarding sterilization and disposal‚ risks include an open flame used to sterilize tweezers as well as chemicals used for disposal
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to gushing problems Hammond‚ J.‚ Brennan‚ M. and Price‚ A. (1999). The control of microbial spoilage of beer. Harper‚ D.R. (1981). Microbial contamination of draught beer in public houses. Proc. Harper‚ D.R.‚ Hough‚ J.S. and Young‚ T.W. (1980). Microbiology of beer dispensing systems Hayashi‚ N.‚ Ito‚ M.‚ Horiike‚ S. and Taguchi‚ H. (2001). Molecular cloning of a putative divalent-cation transporter gene as a new genetic marker for the identification of Henrikkson‚ E. and Haikara‚ A. (1991). Airborne
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Project 1: Antimicrobial Activity of Soil Isolates John Franklin Farrar Department of Biology and Microbiology and Biology Address BOX 22750 BOWEN HALL‚ RALEIGH‚ NC‚ 27607 Abstract: Isolation and characterization of microorganisms is a practice that aids in Increasing ones knowledge of a laboratory setting and it helps improve on Using sterile technique. Isolates of soil microbes can be categorized and Characterized based on a number of criteria ranging from gram-staining
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ANTIMICROBIAL ACTIVITY OF SOIL USED IN THE TREATMENT OF BACTERIAL DIARRHOEABYSITUMBEKO LIWELEYA(s213459531)Research Proposal Submitted in fulfilment of the requirements for the degree of BTECHBIOMEDICAL TECHNOLOGY | | FACULTY OF HEALTH SCIENCES | atNelson Mandela Metropolitan University | MODULE- RESEARCH METHODOLOGYDUE DATE- 16th MAY‚ 2013.SUPERVISOR- PROFESSOR N. SMITH | | Contents ANTIMICROBIAL PROPERTIES OF SOIL USED IN THE TREATMENT OF BACTERIAL DIARRHOEA
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