API 20E incubation tray‚ Bunsen burner‚ oxidase reagent and bacteria Methods: The method used for the API (analytical profile index) 20E System was the aseptic technique was used for the inoculating loop to get a unknown colony from the streak plate or pure culture slant. A small amount was smeared over the filter paper and several drops of oxidase reagent were added and the color change was noted. Another loop-full of bacteria was added to the test tube containing 5 ml of sterile saline‚ recapped
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this lab you will be adding to that experience by reviewing the differences in cell structure for Prokaryotes and Eukaryotes (previously covered in Lecture Unit #1) and learning about staining of microbes. Part I. Smear Preparation in Mastering Microbiology. Log in to MM‚ go to the Study Area‚ and then to Microlab Tutor “Smear Preparation”. After viewing the short video‚ answer the following question: 1. List the major steps in smear preparation If the slide is not clean‚ clean the slide. Label
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cycle. Soil sample was collected and diluted to volume. A measure of 0.6 mL of the soil suspension were put into a 1/3 stength of Hay Infusion Agar. Then‚ 0.4 mL of 24-hour old E. coli was added to serve as nutrient source of the Dictyostelids. The plates were incubated at 24°-25°C for 24 hours and were observed periodically the second day. Using a compound light microscope‚ the development of sorocarps were observed and noted. From the results collected‚ each stage on the life cycle of Dictyostelids
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Bacterial Smears Are Fixed before Staining to? Answer It is important to heat fix the bacterial smear before staining so as to‚ kill the bacteria‚ firmly adhere the smear on to the microscopic slide to prevent washing off during staining‚ and to allow the sample to readily take up the stain. Reference: www2.hendrix.edu What is the purpose of heat- fixing the smear? It helps the cells adhere to the slide so that they can be stained. The purpose of heat fixing is to kill the organisms without
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Some Solutions to Problems (Übungen) to Lecture-1 of Lecture Series “Heat Transfer – 1” Institut für Energieverfahrenstechnik und Brennstofftechnik‚ TU Clausthal‚ SS 2005 1.1 A heat rate of 5 kW is conducted through a cross section area of 20 m2 and thickness of 3 cm. If the inner (hot) surface temperature is 600ºC and the thermal conductivity of the material is 0.5 W/mK‚ what is the outer surface temperature? Solution: Tout = 585 °C 1.2 The heat flux through a wood slab 50 mm thick‚ whose inner
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in order to produce isolated colonies of the organism on an agar plate. The unknown test tube with the bacteria is flame sterilized using a Bunsen burner. Once the bacterium test tube has been flame sterilized‚ a flame sterilized inoculating loop will be used to gather
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Isolation and identification of an unknown bacterium Pillay‚ Esmerelda (209504371) School of Biochemistry‚ Genetics and Microbiology Department of Microbiology University of Kwa-zulu Natal 25 October 2010 ABSTRACT Different types of bacteria in various forms are found all around us‚ and it is a microbiologist’s job to be able to identify these bacteria. Using various staining techniques and physiological tests‚ an isolated bacterium can be identified. In this experiment‚ a single bacterial
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if there are 20 colonies on a plate then you must have placed 20 cells to create these 20 colonies. These cells are therefore referred to as colony forming units or CFUs for short. From the number of colonies and the dilution of the growth on a plate we are able to count the number of microorganism in an original culture. The equation for that: Concentration of culture in CFU/ml = number of colonies on a plate multiplied by the serial dilution on that same plate In this exercise you were browsing
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gel versus the control group will be more effective in killing S. aureus. Another alternate hypothesis is that alcohol gel is more effective in killing S. aureus than the hand soap. Materials and Methods: The bottom of a Trypticase Soy Agar (TSA) plate is divided into three (3) pie sections along with the experimentalist initials‚ class day and class time. A sample of S. aureus is obtained in a closed lid sample tube. The sample tube is
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of my unknown and Salmonella typhimurium found in the Bergey’s manual. Gram staining showed gram negative rods‚ a motility test was also conducted to see if the bacterium moved or not‚ it was found to be none motile. Three different types of agar plates were used‚ they had two known bacterium put on along with the unknown to be able to compare negative and positive results if the known with the results of the unknown‚ refer to Barbaro (2016) for how the test were done. Table 1: Results of unknown
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