Buffer Preparation (Gozani Lab) 1. 1 M Tris-HCl Buffers pH Volume (L) TrisBase (g) HCl (ml) pH 7.0 2 242.2 150-155 pH 7.5 2 242.2 120-125 pH 8.0 2 242.2 80-85 Autoclavable. 2. EDTA 0.5 M (pH8.0) 0.5M‚ 1L: 148 g EDTA + ~30-40 g NaOH to adjust pH (or 186 g EDTA-Na.2H2O + ~20 g NaOH) Note: pH adjusted by NaOH is essential for solubility. Autoclavable. 3. TAE DNA Electrophoresis Buffer (50 X) (2 M Tris‚ 50 mM EDTA) 2L 484 g Tris 114.2 ml glacial acetic acid 200 ml 0.5 M EDTA 8.0 To make
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IDENTIFYING GRAM POSITIVE COCCI As mentioned in Exercise 8‚ “Identifying Gram Negative Rods”‚ identifying bacteria is a common activity in the microbiology lab. Like the game Clue™‚ each time you gather a piece of information to solve the mystery‚ you gather some information that supports some identities and eliminates others from contention. In the lab‚ the process continues as you gather more information until only one microbe remains and all others have been eliminated as possibilities. Thus
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Proteus vulgaris Antibiotic Sensitivity Assay By: David Toelkes BIO 225 June 12‚ 2012 Lab Partners Alexis Blackmon Cheryl Cardiff Megan Dereani Logan Freeman Abstract Development of bacterial resistance to the antimicrobial drugs identified the need to determine a given bacterium’s susceptibility or resistance to a given drug which prompted W. M. M. Kirby and A. W. Bauer to develop a single disk method for susceptibility testing. This experiment used the Kirby-Bauer disk diffusion
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swab containing the inoculum was introduced to the media‚ and using a nichrome inoculating loop‚ the inoculum was streaked using the clock method. The clock method was performed by drawing four different quadrants or sectors on the back-side of the plate containing the culture medium. The four quadrants were drawn in such a way that at the last two lines of every sector there is small portion termed as a tail [2] that is the actual starting point of the next sector. Streaking is performed from the
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of Weight on Diffusion: Molecule Molecular Formula Did it cross selectively permeable membrane Starch Polysaccharide made up of many Glucose molecules No Glucose C6H12O6 Yes The Effect of Weight on Diffusion’s Speed: We put 3 elements in a TSA plate and left them for 30 minutes to see where diffusion is faster‚ the results are: 1. Potassium permanganate KMnO4: Mw= 158.034 g/mol [Fast] 2. Methylene Blue: Mw= 319.85 g/mol [Medium] 3. Malachite Green:
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left leg. To this day‚ he walks with a limp‚ uses a leg brace‚ and has a pump inside his stomach that continually delivers medicine to his leg and back. He has a handicapped license plate‚ gets double-checked by TSA at airports because his pump sets off the alarms‚ and is considering getting serious spinal plate fusion surgery. While I know that my dad is different from the others because of his accident‚ it never occurred to me when I was young that my dad was any less capable‚ less able‚ or
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Part I INTRODUCTION TO MICROBIOLOGY 1 The Evolution of Microorganisms and Microbiology CHAPTER OVERVIEW This chapter introduces the field of microbiology and discusses the importance of microorganisms not only as causative agents of disease‚ but also as important contributors to food production‚ antibiotic manufacture‚ vaccine development‚ and environmental management. It presents a brief history of the science of microbiology and an overview of the microbial world. The origin of life and
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OF SCIENCE DEPARTMENT OF BIOLOGICAL SCIENCES COURSE TILTE: INDUSTRIAL/FIELD ATTACHMENT COURSE CODE: SBL326 NAME: PAMELA K. MUKWEYI REG. No.: BTE/0517/08 DURATION: 9TH MAY – 20TH JULY 2012 SUBMISSION DATE: ATTACHMENT PLACE:CENTRE FOR MICROBIOLOGY RESEARCH- KEMRI Scope/purpose The Industrial Attachment program fulfils part of the requirement in pursuing the degree of Bachelor of Science in Biotechnology in Masinde Muliro University of Science and
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1997. Cellulase degrading enzymes and their potential industrial applications. Biotechnology Advances 15: 583–620. Lee‚ S.M. and Koo‚ Y.M. 2001. Pilot-scale production of cellulose using Trichoderma reesei Rut C-30 in fed-batch mode. Journal of Microbiology and Biotechnology 11: 229-233. Ponnambalam‚ A.S.‚ Deepthi‚ R.S.‚ and Ghosh‚ A.R. 2011. Qualitative Display and Measurement of Enzyme Activity of Isolated Cellulolytic Bacteria. Biotechnololgy‚ Bioinformatic and Bioengineering 1(1): 33-37 Yi‚ J
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BTEC Assignment Brief Programme title BTEC LEVEL 3 Applied Science Extended Diploma Unit number and title 21 Biomedical Science Techniques Assignment title Microbioology Assessor (receiving the work) Nacho Julve-Larrubia Issue date 09/08/13 Deadline(s) (two groups) Task 1 (U21:P1P2) 23/09/13 and 03/02/14 Task 2 (U21:M1) 30/09/13 and 10/02/14 Task 3 (U21:D1)07/10/13 and 24/02/14 Scenario / Vocational Context To reduce the risk of infectious disease it is important
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