knowledge about selective media and differential media. Practice use of the catalase test‚ coagulase and the oxidase test. Observe microbial flora of the nose. Significance: Understand the use of Mannitol salt agar‚ blood agar and MacConkey agar plates which must be used based on the components of the bacteria. The catalase test will be used to understand the difference in facultative anaerobic and groam positive from aero tolerant anaerobes. The coagulase test converts fibrogen to fibrin‚ causing
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will be placed on a 5% blood agar plate or a MacConkey plate‚ depending on the type of organisms plated. These plates are selected because of the ease of differentiation between organisms. The original vial and all diluted aliquots will then be refrigerated to restrict growth until the plates can be processed and evaluated. The plates will be streaked in the standard format for colony count. The plates will be incubated at 37 degrees Celsius for 24 hours. The plates will then be examined for purity
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effect of different pH level of the Agar plate to the antibacterial activity of Santol (Sandoricum koet jape). Specifically‚ the study will seek for the answer of the question: 1.) Is there any difference on the No.of colonies after applying Santol (Sandoricum koet jape) extract on the Agar plate with pH level greater than seven (base)? 2.) Is there any difference on the No.of colonies after applying Santol (Sandoricum koet jape) extract on the Agar plate with pH level less than seven (acid)
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cereus Biol 251 Microbiology 5/14/2009 Introduction The purpose of this study is to differentiate and identify two unknown organisms provided by the instructor in a nutrient broth. It is only known that the two organisms are from vomit; one is gram-positive and the other is gram-negative. It is necessary to first separate the two organisms by inoculating a nutrient agar plate using the streak-plate method. The initial streak-plate procedure was performed and placed
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Cited: Goering‚ Richard V. 2008. Mims’ Medical Microbiology. Elsevier Limited‚ China. Granato‚ Paul A. 2008. Laboratory Manual and Workbook in Microbiology: Applications to Patient Care. McGraw-Hill‚ New York‚ NY.
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UNIVERSITY OF DAR –ES-SALAAM COLLEGE OF NATURAL AND APPLIED SCIENCES DEPARTMENT OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY MC206: FOOD MICROBIOLOGY PRACTICALS PRACTICAL 1 MICROORGANISMS IN THE ENVIRONMENT GROUP #:1 NAME: DUSENGEMUNGU Léonce REG #: 2011-04-07086 COURSE INSTRUCTOR: Dr Mugassa S.T Rubindamayugi
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Staphylococcus aureus (S. aureus) is a gram positive bacterium that when looked at under a microscope it appears to be a cluster of what looks like purple circles. This shape is known as cocci. When grown on a TSA plate‚ Staphylococcus aureus appears to be yellow to opaque in color. S. aureus is known as one of the most resistant bacterium to multiple antibiotics and considered the most pathogenic. Everyone is susceptible to S. aureus with one way of transmission being from foods such as chicken
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incubation‚ results were collected as growth or no growth for each temperature. Methods and Materials: Figure 1a: agar plate Materials 6 nutrient agar plates media grown overnight in Tryptic Soy Broth E. coli P. fluorescens B. stearothermophi lis Inoculum loop Divide
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contaminated during the gram stain process by another person in the laboratory.} [I do think that the gram-stain result is surprising - the two gram-positive cocci species we’ve been given form medium-sized‚ round‚ uniformly colored colonies on TSA plates. When I say the borders of the bacterial colonies are irregular‚ I mean that both the shape is irregular and the consistency of the borders is not like the center of the colony. I associate this type of appearance with rod-shaped bacteria
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morphology. Colonies can be classified according to their colour‚ form‚ elevation‚ margin and size. Pure cultures of microorganisms are those that are uniform and are of the same descendants of the same organism. The isolation of colonies by streak plate technique allows the obtainment of pure cultures which can be studied further. Good quality aseptic technique and proper sterilisation are a required to ensure that all samples produced are free from unwanted organisms that can lead to contamination
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