Dyes by TLC Pre-lab Properties: Ethyl acetate‚ ethanol‚ silica‚ azobenzene‚ azulene‚ 4-(p-nitrophenylazo) resorcinol‚ methyl red‚ bromocresol green (solubilities in water and ethanol) Purpose: To identify compounds from an unknown mixture using TLC Up to 100% of missed points can be recovered from this lab Watch the video: http://www.youtube.com/watch?v=e99nsCAsJrw (MIT) TLC plates are near the main hood DO NOT BREAK CAPILLARY TUBES Keep spots small and concentrated (video) Stop TLC before solvent
Premium Thin layer chromatography Chromatography Solvent
Expt. 8.1 Thin Layer Chromatography (TLC) Analysis of Analgesics Krista Jinks CH 211L Section F 10/27/14 I. Abstract: The objective of this experiment was to analyze analgesic tablets by TLC to determine what analgesics they contain. The analgesics that could be in the tablets included ibuprofen‚ acetaminophen‚ aspirin‚ and caffeine. Through the use of TLC‚ unknown substance #62 was found to be Bayer Aspirin because it only contained aspirin. II. Introduction: 1. Briefly describe
Premium Thin layer chromatography Chromatography
A- HPTLC Flavonoid profile of methanol extract M. concanensis leaves B- HPTLC Flavonoid profile of methanol extract M. concanensis flowers C- HPTLC Flavonoid profile of methanol extract M. concanensis seeds 4.2.3.1 C) HPTLC Phenol profile of M. concanensis Nimmo. HPTLC finger printing of M. concanensis was done by using selected solvent system Chloroform: Ethyl acetate: Formic acid (50%:40%:10%v/v) for leaf‚ flower and seed extracts‚ visualized under UV 254 and 366 nm showed more
Premium Carboxylic acid Alcohol Acetic acid
Experiment 3: The preparation of acetaminophen (paracetamol) with thin layer chromatography (TLC) to monitor the reaction. Abstract: This experiment is to demonstrate the preparation of paracetamol and its properties. Reflux and filtration of 4-aminophenol and acetic anhydride formed the crude sample. Further analysis of dry white crystals were used to give quantitative measurements and a percentage yield of 46% was obtained. The overall conclusion is that the acetic anhydride reacted with the
Premium Functional group Thin layer chromatography Acetic acid
factors that might affect the percentage yield‚ such as influence of the harvest place‚ harvest period‚ type of soil and climate. 4.2 Phytochemical analysis 4.2.1 Phytochemical compounds determination Saponins‚ alkaloids‚ tannins‚ flavonoids and Phenolic compounds were qualitatively analyzed. The summary of phytochemical analysis results of aqueous crude extracts of C. nardus leaves was tabulated in table 4.1. Table 4.1. Phytochemical analysis results of aqueous crude extracts of
Premium Antioxidant Antioxidant
Thin Layer Chromatography (TLC) 5.0 Detection and Identification 7 6.4 UV-Vis Spectrophotometry 6.5 Mass Spectrometry 6.6 Nuclear Magnetic Resonance Spectrometry 6.0 Health Benefits of Daidzein 18 7.7 Exposure to Daidzein 7.8 Anti-Tumour Effect of Isoflavone 7.9 Daidzein and Cardioovascular Disease 7.0 Conclusion 21 8.0 References 22 1.0 Introduction Flavonoids are present in plants as mixtures
Premium Mass spectrometry Chromatography Soybean
Chromatography – Analyzing Analgesics by TLC and Isolation of β-Carotene by Column Chromatography Introduction/Background: Flavonoids are an important group of additives that can be defined as pure substances either natural‚ extracted from raw materials or synthetic. Chromatography is the separation of two or more compounds or ions caused by their molecular interactions with two phases – one moving and one stationary (Weldegirma 2012). Three types of chromatography are used
Free Chromatography Thin layer chromatography
ether‚ methanol‚ distilled water‚ and ethanol solvents. Evaluation of phytochemicals such as alkaloids‚ flavonoids‚ carbohydrates‚ glycosides‚ protein and amino acids‚ saponins‚ and triterpenoids revealed the presence of most of the constituent in polar extracts such as ethanol‚ methanol‚ and aqueous extracts compared with nonpolar extracts (petroleum ether and chloroform). However‚ flavonoids‚ proteins and amino acids‚ tannins‚ and phenols were found to be universally occurring in all the extracts
Premium Solvent Solvent Bacteria
Larvicidal Activity of Lantana camara L. Leaf Extract Liquid Fractions Against Aedes aegypti I. Introduction • Start with malaria/dengue statistics for dramatic effect‚ then transition to the title of the study • Include in-‐text citations • Include the studies & concentrations used by these studies at Slide 11 • Replace “chemical” with “synthetic”
Premium Concentration Chemistry Chemical equilibrium
chloroform (1:1 v/v). All the three extractions were pooled and concentrated in vacuum at 40°C using a rotary evaporator. The crude extract was then diluted in minimum amount of chloroform (2ml) and citrinin was estimated by thin layer chromatography (TLC) according to Razak et al.[16] Subsets of citrinin obtained were estimated by High performance Liquid Chromatography (HPLC). The control contained PDB broth and 10 μl of fungal suspension. Briefly‚ different volumes (1 to 5 µl) of sample extracts were
Premium Chemistry Water Oxygen