1 Column and Thin Layer Chromatography: The Separation of Spinach Pigments Russ Hoburg 02/15/2012 Abstract The main goal in doing the column and thin layer chromatography was to separate spinach extract into its components based on polarity and then to analyze the components. The separation of the spinach extract was done using the column chromatography with the wet/slurry packing method. Alumina was inserted into the column to act as the stationary phase. After the components were separated
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Column and Thin Layer Chromatography: The Separation of Spinach Pigment Abstract Spinach extract was separated into fractions containing compounds of similar polarity by column chromatography. Based on solid-liquid phase partitioning‚ this separation technique exploited the different polarity of the compounds in the spinach extract. Three fractions with different colors were obtained. The extract and its fractions were analyzed using thin layer chromatography (TLC). The TLC results showed that
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Title Identification of Analgesic Compounds Using Thin Layer Chromatography Abstract The objective of laboratory experiment six was to identify an unknown analgesic tablet using Thin Layer Chromatography (TLC) analysis. The procedure involved preparing TLC plates‚ solubilizing standard analgesics (ibuprofen‚ aspirin‚ acetaminophen‚ caffeine) and the unknown in isopropyl alcohol (IPA)‚ and developing the plates in acetone. Rf values were calculated for each compound. The unknown tablet showed an Rf
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Film discussion: Thin Anorexia nervosa is an eating disorder that occurs when an individual is unrealistically concerned about being overweight or feels an overwhelming need to be so thin that in either case an individual eats so little that they become malnourished. While Bulimia is where an individual binges and purges. They may eat a lot of food at once and then try to get rid of the food by vomiting‚ using laxatives or sometimes over-exercising. Both of which are diseases linked to physical
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Chromatographic Separation of Fluorene and Fluorenone Abstract: A mixture of Fluorene (1) and Fluorenone (2) was separated by column chromatography. Chemical Equation: Fluorene (1) Fluorenone (2) Mp 114C mp 83C MW 166.22 MW 180.21 Introduction: Column chromatography was invented by Tswett in 1906 so that his study of the chemistry of chlorophyll could be facilitated. Tswett made significant advances in the method and demonstrated its applicability to the separation
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Chromatographic conditions Shimadzu VP-ODS column is (250 mm x 4.6 mm i.d.‚ 5 µm particle size)‚ Shimadzu‚ Kyoto‚ Japan. The mobile phase consisted of a mixture of 0.3% TEA‚ 10% n-propanol in a solution of 0.1 M SDS adjusted to pH 2.8 using 0.02 M orthophosphoric acid. It was pumped at a flow rate of 1 mL min-1. The column was kept at ambient temperature and the wavelength of the detector was at 225 nm. Standard solutions Stock solutions of LVD‚ CBD and ETC containing to 1.0 mg mL-1 were prepared
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Upon first examining the title of this poem‚ “The Layers‚” I predicted that the poem would be about a person’s “layers‚” such as their personality‚ and who they present themselves to be on the outside versus who they really are on the inside. After reading the poem‚ I found the title in the line “Live in the layers‚ not on the litter.” In context of the poem‚ I’d say this line and the title are using layers to represent different parts of our lives. Throughout the poem‚ the speaker is exploring and
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Into Thin Air Literary Analysis Everest will devour all in its icy crevasses and leave one’s thoughts twisted‚ questioning why they came here in the first place. Into Thin Air‚ a journalistic view novel by Jon Krakauer‚ tells of the May 1996 tempest that ominously shadowed Everest‚ leaving all on the summit oblivious as the storm’s winds growl from a short distance below them. On May 11th when the storm attacks at its full strength‚ it would leave eight people dying during their summit attempt. Three
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in a forest with nobody present‚ does it make a noise? Does the moon exist when there’s no-one to look at it? And is the hallway behind me still an ominous jumble of ornate casements and baleful oil paintings‚ or has it turned into something else? Layers of Fear makes space for plenty of gristle and gore during its five hour playtime‚ but the game’s greatest weapon is simply the dread of objects misbehaving when left unobserved. This is an anxiety games in general are well-placed to exploit - game
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“Brilliant. Vertiginously exciting…vibrantly imagined. An achievement of extraordinary depth and beauty of the natural world.” –Raul Dagmar Into Thin Air is based on a true story seen through the eyes of Jon Krakauer. He is a journalist and mountain climber who decides to climb the treacherous Mount Everest and joins the most disastrous expedition in history. Krakauer mixes in with the climbing service called Adventure Consultants‚ which is guided by Rob Hall who is also responsible for the lives
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